Since 2007, diamide insecticides have been widely used in Korea to control various types of lepidopteran pests including Spodoptera exigua. For nearly a decade, diamide resistance in field populations of S. exigua across 18 localities has been monitored using bioassays. Based on the LC50 values, some field populations showed a high level of resistance against chlorantraniliprole, although regional and temporal variations were observed. To investigate resistance at a molecular level, mutations (Y4701C, I4790M, and G4946E) were examined in the ryanodine receptor (RyR), which is the primary mechanism underlying diamide insecticide resistance. As a result, only I4790M mutation was found in most of field populations. As resistance levels varied significantly despite the uniform presence of the I4790M mutation, we considered the presence of another resistance factor. Further, the I4790M mutation was also found in S. exigua specimens collected prior to the commercialization of diamide insecticides in Korea as well as in other countries, such as the USA. This finding led us to hypothesize that the I4790M mutation were predisposed in field populations owing to selection factors other than diamide use. For further clarification, we conducted whole-genome sequencing of S. exigua (449.83 Mb) and re-sequencing of 18 individuals. However, no additional non-synonymous mutations were detected in the RyR-coding region. Therefore, the high level of diamide insecticide resistance in Korean S. exigua is not caused by mutations at the target site, RyR, but is attributed to other factors that need to be investigated in future studies.

최근 파밤나방 등 다양한 나비목 해충에서 diamide 계통의 살충제 (IRAC group 28)의 저항성이 많이 보고가 되고 있다. 국내에서도 이미 배추좀나방과 파밤나방의 저항성이 보고되었는데, 본 연구에서 사용된 강릉에서 2018년 채집한 파밤나방의 경우 Chlorantraniliprole에 대하여 감수성 대비 2,500배 이상의 높은 저항성을 나타냈다. 추천 농도 처리 시 30% 이하의 방제가를 나타냈으며 Cyantraniliprole, Cyclaniliprole, Flubendiamide에서 높은 교차저항성을 나타냈다. 이 저항성 집단에서 diamide 계통 살충제의 작용점인 ryanodine receptor에 이미 보고된 G4946E 돌연변이는 발견되지 않았으나 새로운 I4760M 돌연변이를 발견하였는데, 2014년 배추좀나방에서 보고된 돌연변이와 위치가 동일하였다. 따라서 I4760M 돌연변이는 파밤나방에서 diamide 저항성에 관여 할 것으로 판단한다.

Nitric Oxide (NO) is an important signaling molecule in the nociceptive process. Our previous study suggested that high concentrations of sodium nitroprusside (SNP), a NO donor, induce a membrane hyperpolarization and outward current through large conductances calcium-activated potassium (BKca) channels in substantia gelatinosa (SG) neurons. In this study, patch clamp recording in spinal slices was used to investigate the sources of Ca²+ that induces Ca²+-activated potassium currents. Application of SNP induced a membrane hyperpolarization, which was significantly inhibited by hemoglobin and 2-(4-carboxyphenyl) -4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide potassium salt (c-PTIO), NO scavengers. SNP-induced hyperpolarization was decreased in the presence of charybdotoxin, a selective BKCa channel blocker. In addition, SNP-induced response was significantly blocked by pretreatment of thapsigargin which can remove Ca²+ in endoplasmic reticulum, and decreased by pretreatment of dentrolene, a ryanodine receptors (RyR) blocker. These data suggested that NO induces a membrane hyperpolarization through BKca channels, which are activated by intracellular Ca²+ increase via activation of RyR of Ca²+ stores.

세포내 칼슘은 다양한 세포에서 중요한 생리적 반응을 일으키며, ruthenium red와 ryanodine은 중요한 칼슘 조절자로 작용한다. Ruthenium red는 세포내 칼슘 저장고에서의 calcium induced calcium release(CICR)를 저해한다. Ryanodine은 ryanodine 통로를 통한 칼슘 방출을 촉진한다. 본 실험은 두 조절자가 생쥐 난자와 초기배아의 세포내 칼슘이온 농도에 영향을 미치는지 여부와 그 유효농도를 알