Background: At present time, smartphones have become very popular and powerful devices, and smartphone applications with the good validity have been designed to assess human balance ability. Objects: The purpose of this study is to evaluate the feasibility of smartphone acceleration in the assessment of postural control ability for six different conditions. Methods: Twenty healthy college-aged individuals volunteered. Static balance ability was measured twice with one-day interval using smartphone application and 3D motion capture system under the six different conditions. Results: Dominant frequencies for each test condition did not show significant differences except for two conditions. The intra-rater correlation coefficient between the first and second tests showed high correlations in six conditions(r>.70, p<.05). Smartphone acceleration and the acceleration calculated from the 3D marker position data showed high correlation coefficient(r>.80, p<.001). Conclusion: Acceleration recorded from a smartphone could be useful assessment variables for balance test in the clinical field.