In the article by Im et al., that appears in the International Journal of Oral Bilogy 2013;38(4):155-160. In page 155, Yoon Kyung Imshould be changed as Yun Kyong Lim.

The major issue in the development of nucleic acid based therapeutics is the inefficient delivery of these agents into cells. We prepared cholesterol conjugated spermine and evaluated its usefulness as a delivery modality for antisense oligonucleotides in HeLa-Luc cells. A 2’-O-methyl antisense oligonucleotide sequence, designed to correct splicing at an aberrant intron inserted into a normal luciferase reporter gene, was used for complex formation with cholesterol conjugated spermine. Effective delivery of this antisense agent into nucleus would results in the expression of a luciferasereporter gene product. The cholesterol-spermine formed stable complexes with the antisense oligonucleotide and showed modest delivery activity. Furthermore, this delivery activity was maintained even in the presence of serum proteins, mimicking in vivo conditions. Cholesterol-spermine thus has potential as a delivery system for antisense oligonucleotides into cells.

This study was conducted to determine the optimum conditions for the treatment of polyamine to promote pollen germination and improve the fruiting rate by overcoming fertilization defects in low-temperature strawberry cultivation. The optimum temperature for pollen germination of strawberries was 30 for Seolhyang and 25 for Maehyang, and Seolhyang had a higher pollen germination rate than that of Maehyang. The addition of polyamines, namely spermine and putrescine to the medium improved the pollen germination rate and spermine treatment showed a better effect than putrescine treatment. The proper polyamine type and treatment concentration for enhancing the potency of pollen germination was 500 μM of spermine for both Seolhyang and Maehyang, which improved the pollen germination rate by 19-23% compared with that of the control. However, combined treatment of spermine and putrescine, resulted in a lower germination rate lower than that of the single treatment. Our results indicated that the treatment of polyamines during flowering in protected cultivation of strawberrise can improve the fruiting rate by overcoming the problem of poor pollen germination due to low temperature.

The present study was carried out to investigate the effects of spermine treatment on the growth, yield and quality in strawberry under low-temperature condition, and thereby develop a chemical method to minimize damages by low temperature in greenhouse cultivation. Spermine treatment significantly improved the growth of strawberry in terms of leaf number, leaf area, leaf length, leaf diameter, plant height and plant weight. The highest effect was observed in the 250 uM spermine treatment and the effect tended to be maintained during the entire growth period of 90 days. Fresh weight and dry weight were significantly different depending on the concentrations of spermine. Strawberry plants treated with 250 uM spermine showed higher fresh weight and dry weight compared to untreated control plants during the growth period. Fruit weight, fruit length and fruit diameter were relatively higher when treated with 100 μM spermine compared to other treatments. The fruit yield was the highest with 14 fruits per plant at 250 μM spermine treatment and the coloration of the fruit was the best at this treatment with the Hunter a and b values of 46.56 and 28.75, respectively. The hardness of strawberry fruit tended to increase higher than 2N at 250 μM and 500 μM 250 uM spermine treatment. The sugar content of strawberries treated with 250 μM spermine was 9.5 ° Bx which was 1.6 ° Bx higher compared to that in untreated control. However, spermine treatment did not affect the acidity of fruit and it remained 0.68-0.76% regardless of treatment concentrations. These results suggest that spermine treatment has a positive effect on the growth and productivity of strawberry fruit under abnormal low-temperature condition. The positive effect was the highest at 250 μM spermine treatment and gradually decreased in the order of 100 μM, 500 μM, and untreated control.

Developmental changes of chlorophyll-protein complexes (CPs) and plastid membrane proteins in greening mung bean cotyledons and the effect of spermine thereon were examined by SDS-polyacrylamide gel electrophoresis. The changes in the amounts of CPs became larger with the progress of greening and light-harvesting chlorophyll a/b protein (LHCP) was the main CP in the early greening stage up to 48 h. As the greening proceeded, chlorophyll-protein of the photosystem I (CPI) accumulated. Application of spermine were effective in accumulating CPs of the thylakoid membrane in the early phase of greening. In the profiles of the plastid membrane proteins, quantitative and qualitative changes were observed with the onset of greening up to 72 h. 56 kD protein of major intensity was observed in all greened chloroplasts and 24 kD protein increased remarkablly in both control and spermine-treated cotyledons. The thylakoids from spermine-treated cotyledons showed higher amounts of thylakoid proteins as compared to the controls. The results suggest that spermine may play a role in the regulation of plastid development and stabilizes the membrane function during greening.