Recent university students are under a lot of stresses due to academic performance, employment, and anxiety about the future in the fierce competition. Mobile games can be used as a plan which can reduce the stresses of these students. However, if they play too long the games, it will cause another big problems. In this paper, we experimented to look for the best mobile game time to alleviate the stresses of university students as follows. First, we chose 16 people which have more stress load than the average student through stress tests by the basic diagnosis questionnaire. Second, we did total eight experiments on the stresses of the subjects in the study. That is, the experiment was carried out once before the test, 6 times for the mobile game (60 minutes), and once before the experiment. Third, we did T-test and multivariate analysis on the collecting data. As a result, it is proved that the mobile game for about 20 minutes could derive the effect on reducing stresses.