The aim of this study was to perform a comparative analysis of tooth brushing patterns before and after education with a newly invented toothbrush. All participants performed toothbrushing on left lower first molar teeth for 10 seconds, using Modified bass method with the Newly invented toothbrush before education. After recording the brushing pattern, the participants were randomly classified into 1) Tell, 2) Tell-Show and 3) Tell-Show-Do group. After their education, toothbrushing patterns were recorded again. A comparative statistical analysis with a professional group was done by a one sample t-test. Each motion of toothbrushing was represented by Roll, Pitch and Yaw directions and pressure. After toothbrushing education, the Pitch, Roll direction and the value of pressure in all groups showed significantly similarity to the professional group. The Yaw direction appears to be improved after education, but the delineated motion was not considered to represent the Modified bass method. The patterns of each group were similar to the professional group as follows; Tell < Tell-Show < Tell-Show-Do. There was general evidence of higher educational effects by telling, monitoring and practical training. Finally, toothbrushing patterns were able to be analyzed by the Newly invented toothbrush correctly and quickly. This will contribute to the establishment of correct toothbrushing habits through public use.