The prolonged bisphosphonate (BP) uptakes are frequently resulted in BP-related osteonecrosis of jaws (BRONJ). The previous study reported that the BP-involved bones were stained blue by Masson trichrome and showed weak birefringence compared to the normal bone1). Using the representative twenty cases of BRONJ osteomyelitis the present study examined the ultrastructure of BP-involved bone by scanning electron microscope (SEM) using decalcified bone microsections. As the BP-involved bones showed different features from adjacent normal bone by blue staining in Masson trichrome method and by rare birefringence under polarizing microscope, the ultrastructure of BP-involved bone matrixes were distinguishable histologically in comparison with normal bone. The normal bone showed the tight attachment of interdigitating dendritic bone matrixes, producing many Haversian canaliculi, while the BP-involved bone showed the compact alignment of granular bone matrixes, resulted in the abortive Haversian canaliculi. The osteocytes in the lacunar spaces of BP-involved bone became shrunken and necrotic, and the BP-involved bone showed many tunnel-like spaces produced by direct chemical resorptions and proteolytic degradation of bone matrixes. Taken together, it was conspicuous that the BP-involved bones were abnormal in their stainability of Masson trichrome, birefringence under polarizing microscope, and ultrastructure under SEM. These findings of BP-involved bone may have an implication for the pathogenetic roles of BRONJ, and can be applicable for the differential diagnosis of BRONJ from other osseous lesions.

Since oral keratinocytes represent the natmal target for HPV(human papill omavi ruses) infecti on, HPV infection may be involved il1 the developmel1t of oral SCC. Through compaJ'ing the morph이 ogic featw-es of NHOK to 다fOK accorcling to calcium concentration by TEM, immortali zed oral keratinocytes(IHOK) transfected by E6/E7 gene of HPV 16 have been gained wide acceptance as a model system for HPV-linkecl oral carcinogenesis. The purpose of this study were to exami ned the ultrastructural fcaturcs of culturcd NHOK, IHOK, and HN4 oral squamous cell CaJ‘CI noma celJ line, and to apply these results to oral carcinogenesis in the future, Prima:rily cul tlU'ed NHOK, IHOK ++ and HN 4 cell line which were cu ltu red under 015 and 12mM CaTT of 1ιBM bulJet kit For tra nsmission electronmi crosco py(TEM). under preconfluency‘ and after 3 days of postconfluency uncler 1.2mM Ca ++‘ cultured NHOK IHOK, and HN4 cell line were immediately fixed in 2, 0% gluta:raldehyde in O.lM cacodylate buffer(pH 7, 4) at 40C 1'01' 1h TEM of cultured NHOK under 1. 2mM Ca ++ showed increased tonofi laments‘ and vaculated ovoid cells wi th cornifi ed envelope, whi le cultured IHOK showed prominent microvilli , unilateral desmosome in microvillus‘ and tonof t!amen ts Under high calcium cu ltured IHOK showed less tonofilaments than that of cultured NHOK, while cu ltu red lHOK a nd HN 4 cell lines showed more increased desmosomes under high calclUm It suggested that the ultrast ru ctura l cha nges of cultured IHOK would be accepted as the morphologic changes of intermediate stage aJl10ng oral carci nogenesis ,

It is very little known that the molecular mechanisms control growth, cell differentiation, and invasion of ameloblastoma into bone. Tissue culture methods have also been used extensively for studies of the cell biology of ameloblastoma. The purpose of this study were to examined the ultrastructural features of ameloblastoma, and to apply these results to examine the pathogenesis of ameloblastoma in the future. Amelobalstoma was primarily cultured under 0.1, 0.15 and 1.2mM Ca++ of KBM bullet kit at 370C and 5% C02. For transmission electronmicroscopy(TEM), cultured ameloblastoma cells were immediately fixed in 2.0% glutaraldehyde in O.lM cacodylate buffer(pH 7.4) at 40C for 1h The ultrathin sections were stained with uranyl acetate and lead citrate, and examined by TEM. The obtained results were as follows. Primay culture ameloblastoma grown in 0.1 mM Ca++ showed interlacing papillary projections without desmosome within early passage(3-4). Primary culture amelobalstoma under high calcium showed prominent desmosomes or tight junctions within early passage. There was evidence of cellular degeneration, as exemplified by nuclear pyknosis, the margination and clumping of the chromatin, and vaculolation under high calcium. The sparse ribosomes, the cytoplasmic space filled with vacuoles, and the condensed mitochondria were seen under high calcium. From the aboving results, under high calcium primary culture ameloblastoma showed rapid cellular degeneration within early passage, indicating that the cells were gradually losing metabolíc actívitíes, leading to enventual cell death. It was thought that it would be necessary to establish cultured immortalized amelobalstoma cell line for studying the pathogenesis of odontogenic tumors.

be involved in the development of oral SCC. If we compare the morphologic features of NHOK to IHOK according to calcium concentration by TEM, IHOK have been gained wide acceptance as a model system for HPV-linked oral carcinogenesis. We already have established immortalized oral keratinocytes(IHOK) transfected by E6 and E7 gene. The purpose of this study were to examined the ultrastructural features of cultured NHOK, IHOK, and HN4 oral squamous cell carcinoma cell line, and to apply these results to oral carcinogenesis in the future. NHOK from healthy retromolar pad was primarily cultured at 37oC and 5% CO2. IHOK, and HN 4 cell line which were cultured under 0.15 and 1.2mM Ca++ of KBM bullet kit. For transmission electronmicroscopy(TEM), under preconfluency, and after 3 days of postconfluency under 1.2mM Ca++, cultured NHOK, IHOK, and HN4 cell line were immediately fixed in 2.0% glutaraldehyde in 0.1M cacodylate buffer(pH 7.4) at 4OC for 1h. The ultrathin sections were stained with uranyl acetate and lead citrate, and examined by TEM. The obtained results were as follows. 1. TEM of cultured NHOK under 1.2mM Ca++ showed increased tonofilaments, and vaculated ovoid cells with cornified envelope, while cultured IHOK showed prominent microvilli, unilateral desmosome in microvillus, and tonofilaments. 2. TEM of HN 4 cell line sowed numerous microvilli, increased N/C ratio, and lateral desmosome in microvilli under 0.15mm, while under 1.2mM well forming desmosomes. From the aboving results, under high calcium cultured IHOK showed less tonofilaments than that of cultured NHOK, while cultured IHOK, and HN 4 cell lines showed more increased desmosomes under high calcium. It was suggested that the ultrastructural changes of cultured IHOK would be accepted as intermediate stage cells for studying oral carcinogenesis.

본 연구는 서로 다른 수명 패턴을 갖고 있는 누에성충 중 두가지 타입을 이용하여 수행되었다. 장명품종(성충 수명이 15일 이상, LLS), 단명 품종(성충 수명이 5일 미만, SLS). 누에성충의 노화 생리를 밝히기 위해 장단명 암수에 있어서의 지방체 미세구조를 비교해 보았다. 단명품종에 있어서는, 암컷에는 조면소포체 및 글리코겐 과립이 세포질내에서 다량 확인된 반면, 수컷에는 활면소포체만이 세포질내에서 발견되었다. 또한 단명품종에 있어서는, 성충 3일째 이후 미토콘드리아의 용적이 비대해지는 경향을 보였으며, 많은 지방구 퇴화가 관찰되었다. 단명품종에 반해 장명품종에서는, 성충 5일째의 암컷은 비교적 정상적인 미토콘드리아와 핵막이 관찰되었다. 성충 15일째에 이르러서야 대부분의 세포막이 사라졌고 미토콘드리아가 비정상적으로 비대해졌다. 장명품종 수컷(성충 10일째)의 세포질내에서 다량의 지방과립이 관찰되었으며, 이 개체는 성충 15일째에 세포내용물이 모두 고갈되어 사망하였다. 따라서, 금후 수명에 관한 조직 연구를 위해서는 조직의 변화상을 관찰하기 용이한 단명품종이 적절할 것으로 사료된다.