본 연구에서는 시판되고 있는 혈청 내 AChE가 acetylthiocholine과 반응하여 GNP에 aggregation 일으키는 원리를 이용하여 신선채소 농산물 중에 저농도 농약을 신속하고 간편하게 분석할 수 있는 비색-신속 농약 검출법을 개발하는 연구를 수행하였다. 먼저 비색-신속 농약 검출법의 최적화를 위해 GNP 입자의 크기에 따른 응집정도 를 확인하여 15~20 nm 직경의 GNP를 선정하였고, 혈청의 희석배수와 acetylthiocholine의 농도를 확인하여 GNP 응집 차이가 가장 큰 혈청 1000배 희석과 acetylthiocholine 1 mM을 최적화 조건으로 선정하였다. 비색-신속 농약 검출법의 평가를 위해 최적화된 비색농약분석법을 이용하여 유기인계 농약은 dimethyl amine으로 카바메이트계 농약은 carbofuran으로 민감도를 분석한 결과 모두 7.5 ng/mL 까지 검출이 가능한 것으로 확인되었으며 이는 기존의 비색-신속 농약 검출법과 비교했을 때 높은 민감도와 특이성을 나타내었다. 농약 이외에 화학물질인 곰팡이독소 등에 대한 반응성은 확인되지 않아 높은 특이성을 나타내었 다. 또한 상추, 깻잎, 양상추에 대한 시료 전처리법을 확 립하고 임의로 오염시킨 3종(상추, 깻잎, 양상추)의 농산물에 대해서 회수율을 확인한 결과유기인계와 카바메이트계 농약을 83.85~133.16% 정도의 회수율이 확인되었다. 이상의 결과 볼 때 본 연구에서 개발한 비색-신속 농약 검 출법을 이용한다면 시판 농산물의 잔류농약을 신속하고 민감도 높게 검출할 수 있을 것으로 판단된다.

Acoustic surveys were conducted in the seas surround the South Korea (South Sea A, South Sea B (waters around the Jeju Island), West Sea and East Sea) in spring and autumn in 2016. First, the vertical and horizontal distributions of fisheries resources animals were examined. In most cases vertical acoustic biomass was high in surface water and mid-water layers other than South Sea A in autumn and West Sea. The highest vertical acoustic biomass showed at the depth of 70-80 m in the South Sea A in spring (274.4 m2/nmi2) and the lowest one was 10-20 m in the West Sea in autumn (0.4 m2/nmi2). With regard to the horizontal distributions of fisheries resources animals, in the South Sea A, the acoustic biomass was high in eastern and central part of the South Sea and the northeast of Jeju Island (505.4-4099.1 m2/nmi2) in spring while it was high in eastern South Sea and the coastal water of Yeosu in autumn (1046.9-2958.3 m2/nmi2). In the South Sea B, the acoustic biomass was occurred high in the southern and western seas of Jeju Island in spring (201.0-1444.9 m2/nmi2) and in the southern of Jeju Island in autumn (203.7-1440.9 m2/nmi2). On the other hand, the West Sea showed very low acoustic biomass in spring (average NASC of 1.1 m2/nmi2), yet high acoustic biomass in the vicinity of 37 N in autumn (562.6-3764.2 m2/nmi2). The East Sea had high acoustic biomass in the coastal seas of Busan, Ulsan and Pohang in spring (258.7~976.4 m2/nmi2) and of Goseong, Gangneung, Donghae, Pohang and Busan in autumn (267.3-1196.3 m2/nmi2). During survey periods, fish schools were observed only in the South Sea A and the East Sea in spring and the West Sea in autumn. Fish schools in the South Sea A in spring were small size (333.2 ± 763.2 m2) but had a strong SV (–49.5 ± 5.3 dB). In the East Sea, fish schools in spring had low SV (–60.5 ± 14.5 dB) yet had large sizes (537.9 ± 1111.5 m2) and were distributed in the deep water depth (83.5 ± 33.5 m). Fish schools in the West Sea in autumn had strong SV (–49.6 ± 7.4 dB) and large sizes (507.1 ± 941.8 m2). It was the first time for three seas surrounded South Korea to be conducted by acoustic surveys to understand the distribution and aggregation characteristics of fisheries resources animals. The results of this study would be beneficially used for planning a future survey combined acoustic method and mid-water trawling, particularly deciding a survey location, a time period, and a targeting water depth.

PURPOSES: This investigational survey is to observe a proper spatial aggregation method for path travel time estimation using the hi-pass DSRC system. METHODS: The links which connect the nodes of section detectors location are used for path travel time estimation traditionally. It makes some problem such as increasing accumulation errors and processing times. In this background, the new links composition methods for spatial aggregation are considered by using some types of nodes as IC, JC, RSE combination. Path travel times estimated by new aggregation methods are compared with PBM travel times by MAE, MAPE and statistical hypothesis tests. RESULTS : The results of minimum sample size and missing rate for 5 minutes aggregation interval are satisfied except for JC link path travel time in Seoul TG~Kuemho JC. Thus, it was additionally observed for minimum sample size satisfaction. In 15, 30 minutes and 1 hour aggregation intervals, all conditions are satisfied by the minimum sample size criteria. For accuracy test and statistical hypothesis test, it has been proved that RSE, Conzone, IC, JC links have equivalent errors and statistical characteristics. CONCLUSIONS : There are some errors between the PBM and the LBM methods that come from dropping vehicles by rest areas. Consequently, this survey result means each of links compositions are available for the estimation of path travel time when PBM vehicles are missed.