This study was carried out to evaluation of biomass generation mechanisms and to propose the estimation method of biomass generation. Agricultural by-product biomass is generated during crops cultivation and after harvest. However these are not uniformly generated yearly and these depending on the seasons. For planning of biomass utilization, accurate information of the biomass resources is needed, especially characteristic and productivity of biomass are necessary. Agricultural by-product biomass are generated in a wide area being scattered and it is one of the major reason why agricultural biomass utilization is not activated compared with other waste biomass. In this study, estimation and evaluation biomass generation is achieved in specific spatial and temporal boundary, A-city in Gyeongi–do and september to November respectively. Quantity and quality of by-product biomass show big difference depending on the crop species and cultivation periods and these difference bring up that accurate biomass estimation should be considered during planning of biomass utilization and technology selecting for biomass converting to energy and other forms.