세계적인 침입해충 담배가루이 (Bemisia tabaci Gennadius)의 한국 계통과 중국 계통의 유연관계를 알아보기 위하여 2019년에 채집한 두 계통들의 biotype 분포, 살충제 반응, 바이러스 보독율을 조사하고 차이를 분석하였다. 미토콘드리아 COI 유전자 서열을 이용하여 집단 분석한 결과 국내는 모든 지역계통에서 Q biotype만 발견되었으며, 중국은 B biotype (14.3%)과 Q biotype (85.7%)이 동시에 발견되었다. 담배가루이 Q biotype의 haplotpye 구성도 중국은 모두 Q1 그룹만 관찰되었고 Q1H1 (79.8%), Q1H2 (20.2%)로 구성되어 있었으며, 한국은 Q1이 우세한 가운 데 Q2도 관찰되었으며 Q1 그룹의 구성도 Q1H1 (1.7%), Q1H2 (97.5%)로 중국과는 크게 달랐다. 15종 살충제에 대한 약제반응은 국내 계통은 일부 약제를 제외하고 대부분 약제에서 충분한 살충력 (mortality≥80%)을 보여주었으나 중국 계통은 40% 이하의 살충력을 보인 약제들이 다수 있었으며 한국보다는 높은 저항성을 갖고 있었다. 토마토 황화잎말림바이러스 (TYLCV)의 보독율은 국내 계통에서는 발견되지 않았으며 중국의 경우 0∼60% (평균 21.4%) 가 발견되었다. 따라서 한국와 중국의 담배가루이 계통 간에는 유전적 조성과 살충제 반응, 바이러스 보독율에 있어서 큰 차이를 보여주었으며 양국의 담배가루이가 서로 다른 유입 패턴을 갖고 있음을 알 수 있었다.

Identifying effective chemical control agents of Bemisia tabaci biotype B and Q is an important step toward IPM strategy. Until 2008, only 10 chemical agents were registered for B. tabaci. From the laboratory screening of 60 insect- and acaricides, 18 chemicals for egg, 10 for nymph and 8 for adult stage were found effective (>90% mortality). Also ten chemicals were less toxic to B. tabaci. Among ten chemicals, some conventional insecticides need further careful resistance monitoring. Field trials with some of the selected chemicals open the possibility to chemical control of B. tabaci biotype Q. Further consideration of non-target effects and resistance development has to be exerted before registration process.

This study was performed to investigate the feeding behavior of Bemisia tabaci B- and Q-biotypes using EPG technique against seven tomato and eight red pepper commercial varieties. EPG waveforms was recorded during three hours compare with the characteristic patterns of feeding behaviors between two biotypes such as total duration of non-probing times, the time taken until stylet activities changed after reaction, number of probes, and total duration of phloem phase. In comparing the effect of the varieties between the two biotypes, biotype Q showed the feeding behavior against all pepper varieties in the total duration of phloem feeding. Daeshin variety has the longest feeding time. However in total duration of phloem feeding, biotype B was observed in hanyeoreumbigarim and Daeshin varieties, but feeding time was very short. Biotype B was longer in total duration of non-probing, and showed lower number of probes, and total duration of probing phase. However, biotype Q was shorter time in total duration of non-probing phase than biotype B, but showed more aggressive probing and stylet pathway pattern. These results suggest that biotype Q are more preferred the pepper host than biotype B. However, tomato varieties between the two biotypes did not show the difference.