To date, the development of anticancer drugs has been conducted using two-dimensional (2D) cell culture systems. However, since cancer cells in the body are generated and developed in three-dimensional (3D) microenvironments, the use of 2D anticancer drug screening can make it difficult to accurately evaluate the anticancer effects of drug candidates. Therefore, as a step towards developing a cancer cellfriendly 3D microenvironment based on a combination of vinylsulfone-functionalized polyethylene glycol (PEG-VS) with dicysteine-containing crosslinker peptides with an intervening matrix metalloproteinase (MMP)-specific cleavage site, the types of MMPs secreted from human hepatocarcinoma HepG2 cells, a representative cancer cell, were analyzed transcriptionally and translationally. MMP3 was confirmed to be the most highly expressed protease secreted by HepG2 cells. This knowledge will be important in the design of a crosslinker necessary for the construction of PEG-based hydrogels customized for the 3D culture of HepG2 cells.

생리활성에 따른 용매분획을 통해 으름(Akebia quinata) 과피로부터 4종의 사포닌을 분리하였다. 으름 과피를 에탄올로 추출한 후 디클로로메탄, 에틸아세테이트, 부탄올 및 물 층으로 순차분획하였으며 분광학적 분석을 통해 부탄올 분획으로부터3-O--L-arabinopyranosyl hederagenin (-hederin), 3-O--L-rhamnopyranosyl (12) -L-arabinopyranoly oleanolic acid (-h