유인 방법은 딸기 묘를 생산하는 전통적인 방법이다. 그러나 딸기 묘를 유인하여 번식하는 방법은 오랜 노동시간이 있어야 하는 노동 집약적인 방법이다. 삽목 방법은 상대적으로 유인 방법에 비해 노동 기간과 작업 시간이 짧으므로 유인의 효과적인 대안으로 주목되어왔다. 이 연구는 유인과 삽목 사이의 노동 기간, 작업 시간 및 묘의 생육을 조사하기 위해 수행되었다. ‘매향’ 딸기는 두 개 동의 딸기 육묘용 유인 온실과 삽 목 온실에서 각각 재배되었다. 유인 방법(유인 작업, 모주 제거, 자묘 간 분리) 및 삽목 방법(삽수 채묘, 저장 전 처리, 삽목 작업)에 대한 주요 작업 시간을 측정했으며, 유인은 158일 동 안 6개의 작업이 필요하며, 삽목은 113일 동안 4개의 작업이 필요했다. 유인은 삽목보다 작업 시간이 더 많이 필요했다. 유인과 삽목묘 육묘 방법에 따른 유의적인 차이가 없었다. 이러한 결과는 묘소질의 손실 없이 육묘 동안 유인 방법보다 삽목 방법이 더 많은 노동 기간과 작업 시간을 절약할 수 있음을 의미한다.

Honey bee, Apis mellifera L., have been widely used as a model organism for biological science because of its highly developed sociality, specialized labor division and passive population management. In order to examine the expression patterns of genes putatively involved in social development in honey bee, quantitative real-time PCR (qRT-PCR) that has been widely used to investigate the expression level of target gene can be used in honey bee study. However, the selection and validation of optimal reference genes is a crucial step prior to running qRT-PCR. In the present study, therefore, the seasonal expression stability of five candidate reference genes in the abdomen of forager and nurse was investigated using three programs (geNorm, NormFinder and BestKeeper), and selected reference genes were validated by the normalization of expression level of vg encoding vitellogenin. Although three programs revealed slightly different gene stability values, overall the combination of two genes (rpS18 and gapdh encoding ribosomal protein S18 and glyceraldehyde-3-phosphate dehydrogenase, respectively) was resulted in the most suitable use for normalization of the target gene in forager. However, a single gene, either rpL32 or rpS18 in the nurse or either rpL32, rpS18, or gapdh in the comparison between foragers and nurses, were suggested to be applied for normalization of seasonal and labor-specific gene expression by qRT-PCR.

Honey bee has been widely used as a model insect for biological sciences because of its sociality and specialized labor division. For the investigation of the seasonal and labor-dependent expression patterns of genes putatively involved in its sociality, quantitative real-time PCR (qRT-PCR) can be applied to quantify gene expression level and selection of reliable reference gene(s) for normalization is an accurate step. In this study, using three softwares (geNorm, NormFinder and BestKeeper), we evaluated seasonal expression stabilities of four reference genes that have been widely used for qRT-PCR in forager and nurse heads. Among four candidates, two genes, rpS18 and gapdh, were suggested to be the optimal reference genes for qRT-PCR.

The purpose of this study was to establish the proper sowing time and planting density of cowpeas for labor-saving cultivation. Experiments were carried out in Naju, Jeonnam Province (Latitude 35° 04' N, Longitude 126° 54' E) during 2012 and 2013. The intermediate-erect type strains used in this study were Jeonnam1 and Jeonnam2. Sowing was performed five times between June 25 and August 5 at approximately 10-day intervals in order to establish proper sowing time, and sowed at 5,000, 10,000, 15,000, and 20,000 plants per 10a to establish proper planting density. The days from sowing to first flowering was shortest (32 days) in plants sowed on July 25 and became longer for plants sowed on or around July 25. The days from sowing to first flowering was longest (41 days) in plants sowed on June 25. The days from first flowering to first maturing was shortest (8 days) in plants sowed on June 25 and, became considerably longer at later sowing dates. The days from first maturing to first harvesting ranged from 8 to 10 days, with little difference among the sowing periods. Plants sowed on August 5 harvested at the same time, and plants sowed between June 25 and July 25 were harvested either three or two times. The yield was highest in plants sowed on July 25: 209 kg/10a was harvested for Jeonnam1 and 221 kg/10a for Jeonnam2. Furthermore, harvested at the same time enabled when the harvesting was delayed for around 15 days because the share of the seeds first harvested was highest (91%). The proper planting density was estimated to be 15,000 plants/10a, showing the highest yields of 199 kg/10a for Jeonnam1 and 224 kg/10a for Jeonnam2.