This study was performed to evaluate the inactivation and microbial regrowth of heterotrophic and nitrifying bacteria using chloramine as a secondary disinfectant for drinking water distribution system. Three sets of the three reactors filled with the Cl2/NH3-N ratio of 3:1, 4:1 and 5:1 were used in these experiments. Chloramine concentration were applied to each set of the reactors with 1㎎/ℓ, 2㎎/ℓ and 3㎎/ℓ, respectively. For the set with 1㎎/ℓ was applied, all the reactors showed that the residual chloramine concentration gradually decreased with elapsed time and reached to zero level after 7 days. Heterotrophic bacteria remarkably increased and nitrification occurred after 11 days. For the sets with 2㎎/ℓ and 3㎎/ℓ, however, the residual chloramine was maintained through the experimenatal period (21 day). Furthermore the regrowth of heterotrophic bacteria and nitrification were not found. More than 2㎎/ℓ of chloramine with Cl2/NH3-N ratio of 3:1, the nitrification could be inhibited by 2 days of contact time.