For developing natural preservatives and insecticide contained in volatile organic compounds of Elsholtzia ciliata and Agastache rugosa, the composition of two plants were analyzed and tested on microorganism survival and insectcide effect from TD-GC-MSD analysis. Main components of Elsholtzia ciliata were elsholtzia ketone, dehydroelsholtzia ketone (82%) and caryophyllene (10%). Main components of Agastache rugosa were estragole (88.2%), dl-limonene (4.5%), and trans-caryophyllene (2%). Inhibition activity of 70% methyl-alcohol extract of A. rugosa against growth of Enterobacter cloacae and Salmonella typhimurium was signifcant effect of observed clear zone and that of E. ciliata was against Pseudomonas aeruginosa, respectively. The mosquito larvae was totally died treated by 0.5g powder of A. rugosa for 3 hours, while 80% of the mosquito larvae was died in E. ciliata powder processing. Mortality of mosquito is the first 5 minutes after the smoked incense treatment showed 65% is E. ciliata and 80% in A. rugos and then finally all mosquito died after 20 minutes.