조직배양묘인 Dtps. pulcherrima (sib.) × Dtps. ‘Kyotoprinty’ 를 이용하여 호접란 체세포 변이체의 형태적 유전 적 변이를 조사하였다. 잎과 꽃에서의 색깔과 형태 변이 도 다양하게 관찰되었다. 조직배양시에 발생하는 잎의 변이와 꽃의 변이중에서 대표적인 개체들 13개를 골라 정상적인 개체와 함께 total genomic DNA를 분리하고 RAPD 분석하여 변이체와 정상적인 개체간 DNA 밴드 차이가 나는지 살펴보았다. 그 결과 Operon primer OPO-06과 OPO-07를 이용한 PCR 분석에서 다형성이 관찰되었다. 잎의 변이를 일으킨 개체와 꽃의 변이를 야 기한 개체에서 각각 특이한 DNA Marker를 선발할 수 있었다.

Somaclonal variation, defined as phenotypic and genetic variations among regenerated plants from a parental plant, could be caused by changes in chromosome structure, single gene mutation, cytoplasm genetic mutation, insertion of transposable elements, and DNA methylation during plant regeneration. The objective of this study was to evaluate DNA variations among somaclonal variants from the cotyledonary node culture in soybean. A total of 61 soybean somaclones including seven ~textrmR1 lines and seven ~textrmR2 lines from Iksannamulkong as well as 27 ~textrmR1 lines and 20 ~textrmR2 lines from Jinju 1 were regenerated by organogenesis from the soybean cotyledonary node culture system. Field evaluation revealed no phenotypic difference in major agronomic traits between somaclonal variants and their wild types. AFLP and SSR analyses were performed to detect variations at the DNA level among somaclonal variants of two varieties. Based on AFLP analysis using 36 primer sets, 17 of 892 bands were polymorphic between Iksannamulkong and its somaclonal variants and 11 of 887 bands were polymorphic between Jinju 1 and its somaclonal variants, indicating the presence of DNA sequence change during plant regeneration. Using 36 SSR markers, two polymorphic SSR markers were detected between Iksannamulkong and its somaclonal variants. Sequence comparison amplified with the primers flanking Satt545 showed four additional stretches of ATT repeat in the variant. This suggests that variation at the DNA level between somaclonal variants and their wild types could provide basis for inducing mutation via plant regeneration and broadening crop genetic diversity.