This study was conducted to examine the distribution of arbuscular mycorrhizal fungi (AMF) in the soil grown tomato plants in Damyang districts. We collected twenty one soil samples from the rhizosphere of tomato plants which were grown under structure. Number of spores/g in the soil sized over 500㎛, 355~500㎛, 251~354㎛, 107~250㎛ and 45~106㎛ were 0.01, 0.02, 0.09, 0.9, and 2.0. Total number of spores/g in the fresh soil were 3.02. Mycorrhizal root infection by vesicles, hyphae and arbuscules were 18.0%, 6.0% and 2.0%. To identify the genus of arbuscular mycorrhizal fungi, isolated mycorrhizal spores from the soil grown tomato plants were inoculated into the host plant of sudangrass and mass propagated for 4 months. As a result of identification, mycorrhizal spores were identified as Glomus sp., Gigaspora sp. and Acaulospora sp.