수출용 토마토 하계육묘시 육묘 용기 와 육묘 일수를 달리 함으로써 나타나는 묘소질의 차이를 구명하고, 묘소질이 다른 묘를 정식하여 재배할 경우 생육과 수량에 미치는 영향을 구명코자 하였다. 육묘시의 묘 생육은 폿트 묘가 플러그 묘보다 좋았으며, 폿트 묘는 육묘 일수가 길수록 좋았으나 플러그 묘는 육묘 일수에 따른 차이가 없었다. 정식 60일 후의 생육도 정식 직전과 같은 경향이었다. 1화방 수확 개시기는 폿트 묘가 플러그 묘보다 빨랐으며, 육묘 일수가 길수록 빨랐다. 4개월간 수량은 폿트 묘가 플러그 묘 보다 유의하게 많았는데 수확 2개월까지 초기수량이 현저히 많았다. 폿트 묘는 수확 2개월까지는 45일 묘와 35일 묘는 큰 차이가 없었고 25일 묘가 가장 적었으나 수확 3개월째부터는 육묘일수에 따른 차이가 없었다. 플러그 묘에서는 수확 3개월가지는 35일 묘가 가장 많았고 25일 묘, 45일 묘 순이었으나 수확 4개월째에는 육묘 일수에 따른 차이가 없었다. 따라서 수출용 모모타로 요쿠 재배시, 수출시기와 수출기간에 따라 육묘 용기 용량 및 육묘 일수를 결정하는 것이 좋을 것으로 생각되었다.
The long term goal of this research is to develop an efficient procedure for large scale production of genetically identical or cloned animals. To improve nuclear transpalntation efficiency in the rabbit, this study evaluated the age of nuclear recipient oocytes on the different steps of nuclear transplantation. The ovulated oocytes in different ages were collected from the superovulated does by flushing oviducts with Dulbecco's phosphate buffered saline(D-PBS) supplemented with 10% fetal calf serum(FCS) from 13 to 15, 17 to 20 and 23 to 26 hours after hCG injection. The denuded oocytes were used as nuclear recipient cytoplasm following enucleation by micromanipulation. The blastomeres separated from the 8-cell embryos were used as nuclear donor. The enucleated oocytes receiving a blastomere in the perivitteline space were fused in the 0.28 M mannitol solution at 1.5 kV/cm, 60 sec for three times. The fused oocytes were co-cultured with the monolayered rabbit oviductal epithelial cells in TGM-199 solution with 10% FCS for 72 hours at 37 in a 5% incubator. The cultured nuclear transplant embryos and in vivo developed embryos collected at 72 hours after hCG injection were stained with Hoechst 33342 dye. Their cell numbers were counted under a fluorescent microscope. The results obtained were summarized as follows ; 1. The aged oocytes(20 hrs. post hCG) showed significantly(P<0.05) higher fusionrates(70 ~ 90%) than the recently ovulated oocytes(30.8%) 2. The aged oocytes which were electrically activated and fused at 20 hours developed to blastocyst at significantly(P<0.05) high rate, while none of the recently ovulated oocytes developed to blastocyst. 3. Even though the aged oocytes at 23~26 hours showed higher fusion rate(85.7%), not only they were inadequate to manipulate but also their developmental potential to blastocyst was highly impaired. 4. The developmental potential in vitro of nuclear transplant embryos was significantly retarded than in vivo deveolped embryos.