쥐오줌풀 부정근으로부터 valepotriates와 valerenic acids 함량을 증진시키고자 다양한 elicitor를 처리하였다. 쥐오줌풀 부정근의 생장은 YE 처리구를 제외한 모든 처리구에서 대조구에 비해 감소하였다. 그러나 valepotriates와 valerenic acids 생산은 모든 처리구에서 대조구에 비해 증가하였으며 특히, valepotriates는 100 μM MeJA 처리구에서 12.56 ± 0.78 mg/l, valerenic acids는 1 g/l YE 처리구에서 10.63 ± 1.1 mg/l 로 가장 높았으며, 이는 대조구에 비해 1.6배와 1.8배의 생산성증가를 가져왔다.

We established a practical method for rapid and large-scale production of Valeriana fauriei var. dasycarpa Hara roots by bioreactor culture and confirmed valerenic acids and valepotriates production. We also compared valerenic acids and valepotriates production patterns according to various media conditions. Among the media tested, B5 medium gave the maximum biomass production of 101 g fresh weight, which was a 5.03-fold multiplication rate obtained 4 weeks after inoculation of 20 g of fresh weight. The best production of total valerenic acids (7.86 mg/l) and valepotriates (8.96 mg/l) was B5 medium.