The present study investigated pine trees, which forms a major plantation species in Korea, with the objective of improving the survival rate of pine trees after planting. Growth responses and characteristics were assessed by controlling the level of fertilizer application, which is a basic controlling the growth of pine seedlings, to identify the optimal fertilization treatment. Pine tree seedlings were grown in 104 containers and were examined 8 weeks after planting. Stem height and were measured at 4-week intervals. In terms of fertilization treatment for 1-0 pine seedlings, the treatment group with gradually-increasing fertilizer concentration (500→1000→1000 →1000mg·L-1) had the biggest increase in stem height and diameter at the root. The survey results indicated that the increased concentration treatment group and the gradually-increasing concentration treatment group had more growth compared with that in the fixed concentration treatment group. The gradually-increasing concentration treatment group (500→1000→1000→1000mg·L-1) had the highest total dry matter production. Nine weeks after fertilization, the tips of the pine leaves turned yellow in the fixed concentration treatment group (3000mg·L-1). The same phenomenon was observed in the treatment group in which the concentration was increased to 2000mg·L-1, and in the gradually- increasing concentration treatment group, when the concentration was raised up to 2000mg·L-1. We concluded that the optimal fertilization conditions for producing healthy pine 1-0 seedlings involve fertilizing once a week with Multifeed 19 at 500mg·L-1 during the seedling period, Multifeed 19 at 1000mg·L-1 during the rapid growth period, and Multifeed 32 at 1000mg·L-1 during the maturation period.