Headwater streams are main sources of water, sediments, and organic materials that are transported downstream. Inthis study, we characterized the variability in benthic macroinvertebrate communities according to spatio-temporal variationresponding to the differences of environmental characteristics. We collected benthic macroinvertebrates at five samplingsites along longitudinal gradient on the Jangjeon stream in the Gariwang Mountain at three different seasons (spring, summerand autumn). We identified 93 taxa in 44 families. The dominant species was Gammarus sp. at the upper stream, whileit was Chronomidae spp. at the downstream. Changes of functional feeding guilds was clearly observed along the longitudinalgradient. Species turnover rates was higher in summer than in other seasons. The habitat condition including the landcover of their riparian zone and hydrological factors were major factors influencing on the community composition.