한국식품위생안전성학회지 Vol.27 No.2 (p.109-116)

초임계유체 추출에 의한 생강(Zingiber officinale Roscoe) Oleoresin의 항균활성

Antimicrobial Activity of Ginger(Zingiber officinale Roscoe) Oleoresin by Supercritical Fluid Extraction
키워드 :
ginger oleoresin(GO),antimicrobial activity,supercritical fluid extraction

목차

ABSTRACT
서 론
재료 및 방법
  재료
  에탄올추출
  초임계이산화탄소 추출
  사용균주 및 배지
  시료 전처리
  생강추출물의 항균력 시험 방법
결과 및 고찰
  에탄올 추출비율에 따른 생강 oleoresin 항균성
  온도에 따른 에탄올추출 생강 oleoresin의 항균성
  추출시간에 따른 에탄올추출 생강 oleoresin의 항균성
  초임계이산화탄소 추출에 의한 생강 oleoresin의 항균성
요 약
감사의 글
참고문헌

초록

The study indicated that antimicrobial activity about gram positive and gram negative bacteria of ginger-oleoresin(GO) extract with the condition of ethanol and supercritical fluid extractions. As the concentration of extraction increases, the clear zone of GO ethanol extract also increased dependently. This led the antimicrobial activity of gram positive bacteria to take bigger place than gram negative bacteria especially in Listeria monocytogenes. There was a high antimicrobial activity in E-III treatment where the ratio of the ginger powder extract to ethanol extraction was 1:6. It was quite effective to treat the antimicrobial activity of GO ethanol extract under 80oC and there was not big difference in the intervals which were the extraction time - 1 to 7 hours. The antimicrobial activity of supercritical fluid extract seemed to take the biggest place in Listeria monocytogenes. From the supercritical fluid extract, it was shown the strong ability of antimicrobial activity in the condition with 100 bar 35oC, 250 bar 35oC and 250 bar 65oC. Furthermore, according to the case of solvent extract, there was not any significant difference in the antimicrobial activity with condition of extraction. However, there was significant antimicrobial activity in E-III treatment of 100 bar and 500 bar of extraction pressure, and 35oC and 65oC of extraction temperature.