한국식품저장유통학회지 (Korean J. Food Preserv.) 제17권 제3호 (p.391-397)

마 품종별 메탄올 추출물의 항균, 항산화 및 항혈전 활성 평가

Evaluation of Antimicrobial, Antioxidant, and Antithrombin Activities of the Rhizome of Various Dioscorea Species
키워드 :
yam,antimicrobial,antioxidant,antithrombin,Dioscorea spp

초록

마는 전 세계적으로  오랜 기간 동안 식용 및 약용으로 사용되어 온 중요한 생물자원이다. 본 연구에서는 2009년 경북 안동지역에서 재배된 D. batatas  (경북1호 및 4호), D. alata L.(경북5호 및 6호), D. bulbifera (둥근마) 및 D. nipponica  (부채마)로부터 메탄올 추출물을 조제하고, 이들의 항산화, 항혈전, 항균 활성을 평가하였다. 먼저 수분함량의 경우 마의 종류에 따라  54.7%∼83.2%로 다양하게
Yams  (Dioscorea spp.) have been used as medicinal and edible resources worldwide.  In the present study, methanol extracts were prepared from various kinds of  yam, including D. batatas (Gyeongbuk No. 1 and No. 4), D. alata L. (Gyeongbuk  No. 5 and No. 6), and D. bulbifera and D. nipponica, and the antioxidant,  antithrombin, and antimicrobial activities of these extracts were evaluated.  The water content of the various yams was 54.7~83.2% (D. batatas> D. alata  L.>D. nipponica>D. bulbifera) and the methanol extracts contained  0.59-1.59% (w/v) solid matter (D. alata L.>D. batatas >D.  nipponica>D. bulbifera). The mean concentrations of total polyphenols,  total flavonoids, and total sugars in the different types of yam were 49.82  mg/g, 9.79 mg/g, and 173.81 mg/g, respectively. Analysis of DPPH  radical-scavenging activity showed that D. alata L. Gyeongbuk No. 6 had a  strong antioxidant capacity (IC50=), whereas the other yams  showed reduced levels of activity (IC50=371-). D. alata L.  Gyeongbuk No. 6 showed a strong antithrombin activity against human thrombin.  Upon treatment with a D. alata methanol extract (1.5 mg/mL), thrombin time  was increased 7.35-fold compared with that when methanol alone was used. In an  antimicrobial activity assay, the D. nipponica extract showed moderate  antibacterial activity against Bacillus subtilis, Staphylococcus aureus,  Proteus vulgaris, and Salmonella typhimurium. Our results indicate that  different varieties of Dioscorea spp., including D. batatas, have useful  biochemical attributes, including antioxidant, antithrombin, and  antibacterial activities.