한국식품저장유통학회지 (Korean J. Food Preserv.) 제18권 제4호 (p.590-596)

참나물의 항균, 항산화 활성 및 대장암세포 성장억제 활성 평가

Antibacterial, Antioxidative and Anti-proliferative Activity against Human Colorectal Cell of Pimpinella brachycarpa
키워드 :
antibacterial,antioxidant,anti-proliferative,Pimpinella brachycarpa

초록

참나물은 광범위하게  이용되는 식용산채임에도 불구하고 그 연구는 전 세계적으로 미미한 상태이다. 본 연구에서는 최근 고급 식용 산채로서 각광받고 있는 참나물의  메탄올 추출물을 조제하고, 이로부터 n-hexane, methylene chloride, ethylacetate 및 butanol을  이용하여 순차적 유기 용매 분획물과 물 잔류물을 조제하여 각각의 항산화, 항균 및 대장암세포 생육억제 활성을 평가하였다. 참나물 메탄올  추출물의 71.51%는
Pimpinella  brachycarpa, called as cham-na-mul in Korea, is an edible popular herb.  However, the study of biological activity of P. brachycarpa is still  rudimentary in worldwide. In this study, from the cultivated P. brachycarpa,  we prepared the methanol extract and its subsequent solvent fractions, and  their antimicrobial, antioxidation, and anti-proliferative activities were  evaluated. The fraction yields of n-hexane (H), methylene chloride (EC),  ethylacetate (EA), butanol (B), and water residue (W) from the methanol  extract were 18.71, 0.7, 0.56, 4.57, and 71.51%, respectively. Analysis of  total flavonoid and total polyphenol showed that the EA fraction contained  the highest contents (89.23 and 200 mg/g), and the W residue has the lowest  contents (19.6 and 2.27 mg/g) among the factions. In antimicrobial activity  assay, the EA fraction showed a broad-range antibacterial activity, while the  H fraction is effective against gram positive bacteria. In antioxidation  activity assay, EA and B fraction showed strong DPPH anion and ABTS cation  scavenging activities including reducing power, and Hand MC fraction showed  effective nitrite scavenging activity (71.43~83.82 /mL of ).  In a while, only B fraction showed strong anti-proliferative activity against  human colorectal cancer HCT-116 (166 /mL of ) as a  dose-dependent manner up to 200 /mL. These results suggest that the  EA and B fraction of P. brachycarpa could be developed as functional food  ingredients.