We measured the non-carbon content of single-walled carbon nanotubes (SWCNTs) in SWCNT soot using thermogravimetric analysis. The weight increased percentage by the oxidation of metal in the raw soot is well obtained by TGA graph which was confirmed with ICP-AES, XRD, and XPS. This work will be very useful for the purity precise evaluation of SWCNT with UN-vis-NIR spectroscopy.

1'0 develop an effi cient scaffold fo1' t issue-engineered bone 1'egene1'ation, we evaluated fully in terconnected globular porous bisphasic calcium-phosphate ceramics. Materials and Methods: Biphasic HA/TCP cera mic scaffolds having f띠 l y interconnected globular structure with small fenestrations adopting foaming method were p1'epared. They were evaluated by cytotoxici ty‘ cellular attachment. and theil‘ differentiation in vitro and the hi s tocompatibili ty, osteoconductivity. and ectopic os teoinductive capacity in 띠 vo ， respectively. They have average 400um s ized spherical pores and ave1'age 100um s ized inter connecting interpores with average 85% porosity. They r evealed comparable compression strength with cancellous bone. They were nontoxic and revealed no noxious effect on cellular proliferation and osteoblastic diffe re ntiation. The cul tured cells on scaffolds were well attacbed and proliferated in multi-layers. The increased surface in fully interconnected globular porous scaffold facilitated osteogenic repair by favored cell ular attachment and osteogenic differentia tion with good osteoconductivity. 1n addition. the scaffold-cell constructs induced favorable ectopic bone formati on These findings suggest that the fully interconnected globular porous biphas ic HA/ß -TCP ceramic scaffold formed by foaming method can be a promising bone substitute and a scaffold fo 1' tissue-engineered bone 1'egeneration

Biomimetic apatite deposition behaviors and mechanical performance for as-rolled and annealed Ni-Ti plates were investigated . A good biomimetic apatite formation and excellent mechanical performance of Ni-Ti suggests that Ni-Ti can be an excellent candidate material as orthopedic implants.

Metal/diamond binary composite coatings on Al substrate without grit blasting were deposited by cold spray process with insitu powder preheating. Microstructural characterization of the as-sprayed coatings with different diamond size, strength and with/without Ti coating on diamond was carried out by OM and SEM. The assessment of basic properties such as tensile bond strength and hardness of the coatings, and the deposition efficiency was also carried out. Particular attention on the composite coatings was on the diamond fracture phenomenon during the cold spray deposition and the interface bonding between the diamond and the Fe-based metal matrix.

Transgenic animals production tools have been valuable for research and purpose. The current methods of gene transfer, microinjection and nuclear transfer, which are widely used in transgenic animal production, but all most methods has only had limited success in production of larger species. Here, we report the possibility of a sperm-mediated gene transfer method in porcine embryos. Oocytes were collected from ovaries harvested at a local slaughterhouse were matured in 500 drops of TCM-199 under mineral oil at 38.5 in a humidified atmosphere of 5%CO2 in air. After 42-43h of in vitro maturation oocytes were denuded. for sperm injection into the cytoplasm of the porcine oocytes, sperm suspension in NIM medium are subjected extraction with TritonX-100 before mixing with a green fluorescent gene (GFP). Sperm with Tritonx-100 were prepared by adding TritonX-100 to a final volume of 0.05% in the sperm suspension and mixing by trituration for 60s before two wishes in NIM medium at 2. A(ter wishing, sperm were mixed with TritonX-100 at followed by washes at 2. Sperm were resuspended in ice cold NIM to a final volume of 400 and 2-20ng/ DNA were triturated on ice for 60s. All microinjection was performed in HEPES-buffered CZB medium at room temperature within 2h. After culture in NCSU-23 for 72h, percent of porcine embryos transfected GFP gene are 20.7%(6/29) in 20ng/ sperm-DNA mixed group and other groups were 3.7 %(2/54)and 4.7%(3/67). These data suggests that sperm-mediated gene transfer method should be used to the production tool of transgenic pig efficiently.

담배나방(Heliocoverpa assulta)와 파밤나방(Spodoptera exigua)유충에 대한 유기인계 살충제인 다이아지논과 담배의 산물인 니코틴의 영향을 조사하기 위하여 본 실험을 시행하였다. 다이아지논이 처리된 담배나방유충의 사망률은 파밤나방유충의 경우보다 훨씬 높았으며 니코틴 처리구에서는 이와 반대양상을 나타내었다. 담배나방유충 중장의 cytochrome P-450 monooxygenases (MFO) 활성은 다이아지논 처리구에 비해서 니코틴 처리구에서 더욱 높게 나타났다. 담배나방유충을 기주식물인 담배잎으로 사육하였을 때 다른 화합물과는 달리 대부분의 니코틴은 변화 없이 배설되었다.

이 시험은 도입남양재 원목에 부착 또는 서식하는 유해병해충의 방제를 목적으로 실시하는 해수수침소독에 있어 농약의 소요량기준을 설정함을 목표로 실시한 것으로써 결과를 요약하면 다음과 같다. 1. 우리나라에 도입되는 남양재 원목의 평균 길이는 11.12m이었으며 직경은 69.55cm이었다. 2. 원목의 전재적에 대한 부상재적은 평균 이었다. 3. 원목의 전재적에 대한 부상표면적의 비의 값은 2.3356( 신뢰수준)이었다. 4. 원목의 전재종 대 부상표면적에 대한 표본추출오차는 1본선내 원목간의 오차(0.2087)가 10본선간의 오차(0.2952)보다 적었다. 5. 원목 당 농약의 적정소요량은 수동식살포기를 이용하는 경우 0.368cc, 그리고 동력분무기를 이용하는 경우는 0.295cc이었다. 6. 예를 들어 남양재 원목 을 도입하여 동력분무기로 해수수침소독을 실시하는 경우 농약소요량은 다음과 같이 계산된다. .