Five insecticides (Acrinathrin, Dinotefuran, Emamectin benzoate, Chlorfenapyr and fluxametamide) approved for tomato cultivation were evaluated in Frankliniella occidentalis populations collected from Chungcheong province (Cheongyang, Chungju and Gongju). Leaf dip bioassay was used to evaluate resistance levels (LC50). Bioassays on Acrinathrin demonstrated higher LC50 concentration in evaluated populations. In particular, the Chungju population was 745.61 times the recommended concentration of the insecticide. Other remarkable resistance levels were recorded for the Dinotefuran with 435.06 times and 196.29 times the recommended concentrations for the populations from Chungju and Gongju, respectively. Bioassays for Emamectin benzoate, Chlorfenapyr and Fluxametamide showed low resistance to insecticides in the evaluated populations.

Yellow flower thrips (WFT), Frankliniella occidentalis is mainly controlled using chemical control methods. But the continuous use of chemical pesticides in greenhouse may contribute to development of insecticide resistance. Therefore, in this study, we evaluated the insecticidal activity of eleven insecticides against the WFT occurring in greenhouse pepper cultivation in the Gyeonggi province. The results showed no resistance in treatments with emamectin benzoate, fluxametamide, and flometoquin while high levels of resistance were recorded in treatments with acrinathrin, acetamiprid, and dinotefuran. The Anseong and Yeoju population was more resistant against spinetoram and chlorfluazuron, respectively, than populations from other regions.

단백질의 구조 예측은 생명 과학 및 의약학 분야의 핵심적인 연구 주제 중 하나로, 단백질의 기능 및 상호작용을 이해하기 위한 주요 정보를 제공할 수 있어 다양한 연구가 수행되고 있다. 이러한 연구의 일환으로 최근 Google DeepMind의 AlphaFold2가 등장하였으며, 단백질 구조 예측 성능을 대폭 향상시켜 CASP(Critical Assessment of Protein Structure Prediction)에서 뛰어난 평가점수를 받아 단백질 구조 예측 분야의 최신 기술을 크게 향상시켰다. 이러한 컴퓨터 기반의 단백질의 구조 예측 방법은, 고전적인 방법을 사용하여 직접 단백질 구조를 결정하는 방법 에 비해 매우 정확하고 빠르며 경제적인 비용으로 수행될 수 있어 단백질 구조 예측 및 생리학 연구를 수행하는 연구자들에게 유용한 방법론이 될 것으로 사료된다. 따라서 본 연구소에서는 곤충을 포함한 무척추 자생동물을 연구하는 연구자들을 위해 단백질 구조 예측을 수행할 수 있도록 64Core/128Threads의 CPU, 256GB의 RAM과 6장의 GeForce RTX 3090으로 이루어진 GPU(Graphical Processing Unit) 고성능 컴퓨터 시스템에 AlphaFold2 program을 구축하였다. 최근 인간을 대상으로 한 단백질 구조 예측 연구는 상당한 진전을 보이고 있지만, 곤충을 포함한 자연계의 동물을 대상으로 한 연구는 여전히 미비한 상황이다. 이러한 자생동물자원연구의 확대를 위해 본 연구소에서 구축한 GPU 시스템 및 생물정보학적 분석 방법이 많이 활용되어야 하며, 이를 위해서는 연구자들 의 협력과 참여가 필요하다.

This study investigated the seasonal occurrence of bark and wood-boring Coleoptera in Pinus densiflora (Siebold & Zucc.) (Pinales: Pinaceae), and Larix kaempferi (Lamb.) (Pinales: Pinaceae) stands using multi-funnel traps baited with pine volatiles in Korea. The number and species of bark and woodboring beetles caught in traps baited with ethanol, α-pinene, and ethanol+α-pinene were compared to determine the effective attractants. In addition, the effects of other pine volatiles, such as (-)-β-pinene, β-caryophyllene, (±)-limonene, β-myrcene, and 3-carene, were investigated. A total of 13,134 wood-boring beetles from 150 species were collected from pine and larch stands from 2019 to 2020. Tomicus minor (Hartig) (Coleoptera: Curculionidae) adults were more attracted to traps baited with α-pinene, whereas Xyleborinus saxesenii (Ratzeburg) (Coleoptera: Curculionidae), Cyclorhipidion pelliculosum (Eichhoff) (Coleoptera: Curculionidae), and Phloeosinus pulchellus (Blandford) (Coleoptera: Curculionidae) adults were more attracted to traps baited with ethanol. Hylurgops interstitialis (Chapuis) (Coleoptera: Curculionidae), Shirahoshizo genus group, Rhagium inquisitor (Linne) (Coleoptera: Cerambycidae) and Rhadinomerus maebarai (Voss & Chûjô) (Coleoptera: Curculionidae) were more frequently attracted to traps baited with ethanol+α-pinene than to traps baited with other attractants. The addition of 3-carene to ethanol+α-pinene enhanced the capture of H. interstitialis, R. inquisitor, and Hylobius (Callirus) haroldi (Faust) (Coleoptera: Curculionidae).

Bangladeshi medicinal plants (BMP) have a history of traditional use in treating chronic inflammatory diseases, but a BMP bark’s antioxidant and anti-inflammatory effects remain largely unexplored. This study assessed methanolic extracts’ antioxidant and anti-inflammatory properties from the bark of 15 medicinal plant species native to Bangladesh. The methanol extracts of BMP bark were evaluated for their total antioxidant activity and ability to counteract inflammation induced by lipopolysaccharide (LPS) in RAW 264.7 macrophages. Among the 15 bark extracts from BMP, Albizia odoratissima (A. odoratissima), Engelhardia spicata (E. spicata), and Shorea robusta (S. robusta) showed the highest total phenolic contents and total antioxidant capacity by effectively scavenging free radicals. In particular, these three bark extracts significantly reduced the mRNA expression of LPS-induced inflammatory cytokines and enzymes inducible by inflammation in macrophages. Also, the mRNA expression of NADPH oxidase 2 was significantly suppressed by the three bark extracts in LPS-induced RAW 264.7 macrophages. These results suggest that out of the 15 bark extracts obtained from medicinal plants in Bangladesh, the extracts from A. odoratissima, E. spicata, and S. robusta exhibit substantial total antioxidant capacity by efficiently scavenging free radicals and also inhibit LPS-induced inflammation in macrophages.

Henricia specimens were collected using a dual approach of trimix scuba diving and fishing nets. This inclusive collection encompasses the discovery of two species highlighted in this study and introduces and provides comprehensive descriptions for Henricia kinkasana and Henricia longispina aleutica. The descriptions offered in this study were derived from the thorough examinations of external morphological characteristics. The documentation provides detailed insight into key traits related to the abactinal and actinal skeletons and spines of these newly recorded species in Korea. This comprehensive examination contributes to our understanding of the distinct morphological characteristics defining each species within the genus Henricia.

Abstract Purpose : This study sought to find ways to improve awareness and ethics of opticians' code of ethics. Methods : A survey was conducted on 102 Korean opticians to investigate the awareness and performance satisfaction of the optician's code of ethics, as well as differences according to gender, age, education level, work experience, and workplace. Results : This study found no gender-based differences in opticians’ awareness and satisfaction with the code of ethics. However, age and education significantly influenced these factors, with higher levels observed among opticians in their 20s and 30s, particularly those in the early stages of their careers. Furthermore, a positive correlation was noted between education level and both awareness and satisfaction. The study revealed that opticians with volunteer experience exhibited higher awareness and satisfaction with the code of ethics than those without. This suggests that volunteering, which fosters professional identity and social responsibility, influences opticians’ ethical awareness. Additionally, groups with a strong interest in foreign languages and self-development demonstrated higher ethical awareness and satisfaction. Conclusion : This study underscores the need for enhanced ethical awareness among opticians. It advocates for the development of methods, case studies, and guidelines to address ethical issues not covered by the existing code, thereby revitalizing ethical practices in the profession. In addition, it is judged that active promotion and education on the code of ethics and professional ethics are necessary for the continuing education of opticians. Key words : Awareness, Code of ethics, Performance satisfaction

최근 정원에 대한 관심이 증가함에 따라 자생 식물을 활용 하기 위한 연구가 진행되고 있다. 참식나무(Neolitsea sericea (Blume) Koidz).는 관상용뿐만 아니라 목재로도 이용할 수 있 다. 따라서 이 연구는 참식나무를 정원 식물 소재로 활용하기 위한 대량 증식 기술 개발을 위하여 종자의 발아 특성과 저장 특성에 대해 알아보고자 진행하였다. 2022년 11월 2일 제주 지역에서 채집한 종자를 실험에 사용하였다. 종자의 내·외부 형태 관찰을 한 결과, 종자가 탈리되는 시점에 배가 완전히 발 달한 형태를 가지고 있기 때문에 형태적휴면(morphological dormancy, MD)이 없음을 확인할 수 있었다. 수분흡수 실험 을 통하여 참식나무 종자는 72시간 이후 40% 정도의 수분흡수 율을 보여 내과피의 불투성에 의한 물리적휴면(PY, physical dormancy)이 없는 것으로 판단하였다. 채종 후 곧바로 무가 온 온실에 파종하면 이듬해 5월에 약 60%가 발아하였다. 저 온습윤처리 0, 2, 4, 8, 12주 후 25/15°C에 배양한 결과 최 종 발아율은 각각 0%, 0%, 21.1%, 32.6%, 34.7%였다. GA3 를 0, 10, 100, 1000mg·L-1 농도로 처리한 후 25/15°C에서 배양한 결과, 최종 발아율은 각각 0, 14.5, 22.7, 42.2%였다. 따라서 본 실험의 결과 8주 이상의 저온 처리 또는 GA3 처리 를 통하여 어느 정도 휴면을 타파시킬 수 있었다. 이러한 결과 를 통하여 종자는 intermediate PD를 가지는 것으로 판단 된다. 일부 발아하지 않은 종자는 deep PD를 가지고 있는 것 으로 판단하였다. 후숙 처리 실험의 결과 4주 만에 수분 함량 이 2% 이하로 감소하였고 종자는 전혀 발아하지 않았다. 따라 서 종자의 저장 특성 중 recalcittrant 특성을 지닌 난저장성 종자로 보인다.

본 연구는 국내에서 실내 관상용으로 많이 활용되는 스킨답 서스의 실내재배 시 적정 인공광원, 수경재배 전기전도도 농 도 및 용토를 선발하기 위하여 수행하였다. 실내재배 시 인공 광원은 형광등, 적청 LED, 백색 LED로 처리하였다. 수경재배 시 양액 농도는 EC 1.2, 1.6, 2.0ds·m-1 수준으로 하였고, 재 배용토는 제올라이트, 코코피트, 펄라이트, 황토볼 4가지로 달리하였다. 스킨답서스의 실내 재배 시 줄기 길이는 형광등 과 적청 LED 처리보다 백색 LED 처리에서 길어졌다. 잎의 크기는 적청 LED 처리에서 형광등과 백색 LED 처리보다 커 지는 경향이었다. 엽록소 지수값은 백색 LED>적청 LED>형광 등 순으로 높았다. 수경재배 시 스킨답서스의 줄기길이는 EC 농도가 높아질수록 길어지는 경향이었고, 용토별로 비교하면 제올라이트와 코코피트에서 높은 경향이었다. 엽록소 지수값 도 EC 농도와 비례하여 높아졌고, 용토별로는 제올라이트와 코코피트에서 높았다. 스킨답서스의 생체중도 EC 농도가 높 을수록 높았고, 제올라이트와 코코피트에 높은 경향이었다. 상기 결과들을 종합할 때, 스킨답서스의 NFT를 이용한 실내 재배 시 적정 인공광원으로는 백색 LED를, 수경재배 양액의 농도는 EC 1.6∼2.0ds·m-1를, 그리고 NFT 용토는 제올라이 트나 코코피트가 적합하다는 것을 알 수 있었다.

Concentrated effluent and spent ion exchange resins (IERs) from nuclear power plants (NPPs) were generated prior to the establishment of a disposal facility site and waste acceptance criteria have been temporarily stored at the NPPs because their suitability for disposal has not been confirmed. In particular, at the Kori Unit 1, which was the first to start the commercial operation in South Korea, the initially generated concentrated effluent and IERs are repackaged in large size of concrete containers and stored without provided regulation standard. The concentrated effluent is package as cementitious form in 200 L drums and repackaged in concrete containers, case of the IERs were solidified or dehydrated and repackaged in round concrete container. In this study, we review and propose a disposal plan for concentrated effluent and IERs repackaging drums that have not been confirmed to be suitable for disposal from the first operating nuclear power plant, Kori Unit 1, 2. First, the concentrated effluent was stored in four 200 L drums respectively, and then, it was again stored in concrete container and which was poured on top using grouted concrete. Therefore, the process was required by cutting concrete container for extracting the internal drums at first. Internal radioactive waste should be crushed to the suitable waste criteria and solidified, finally disposal in to the polymer concrete high integrity container (PC-HIC). IER was repackaged and disposal in square type of 200 L concrete drums respectively covered the cap. So, extracting the internal drums should be extracted after removing the cap of external concrete container. Cement solidification drums can be crushed and re-solidified or disposed in the PC-HIC. Stored IER after dehydrated can be disposal in PC-HIC. In conclusion, the container was used as a package that repackaging the concentrated effluent and IER was separated into two different types of waste depending on the level of contamination of radioactivity, the polluted area is disposed of as radioactivity contamination or the unspoiled area will be treated as self-disposal waste.

The WRK (Waste Repository Korea bentonite) compacted bentonite medium has been considered as the appropriate buffer material in the Korean SNF (Spent nuclear fuel) repository site. In this study, hydraulic properties of the WRK compacted bentonite core (4.5 cm in diameter and 1.0 cm in length) as the buffer material were investigated in laboratory experiments. The porosity and the entry pressure of the water saturated core at different confining pressure conditions were measured. The average velocity of water flow in the WRK compacted bentonite core was calculated from results of the breakthrough curves of the CsI aqueous solution and the hydraulic conductivity of the core was also calculated from the continuous flow core experiments. Because various gases could be generated by continuous SNF fission, container corrosion and biochemical reactions in the repository site, the gas migration property in the WRK compacted bentonite core was also investigated in experiments. The gas permeability and the average of gas (H2) in the core at different water saturation conditions were measured. Laboratory experiments with the WRK Compacted bentonite core were performed under conditions simulating the DGR environment (confining pressure: 1.5- 20.0 MPa, injection pressure: 1.0-5.0 MPa, water saturation: 0-100%). The WRK Compacted bentonite core was saturated at various confining pressure conditions and the porosity ranged from 27.5% to 43.75% (average: 36.75%). The calculated hydraulic conductivity (K) of the core using experimental results was 8.69×10-11 cm/s. The gas permeability of the core when the water saturation 0~58 % was ranged of 19.81~3.43×10-16 m2, representing that the gas migration in the buffer depends directly on the water saturation degree of the buffer medium. The average gas velocity in the core at 58% of water saturation was 9.8×10-6 m/s, suggesting that the gas could migrate fast through the buffer medium in the SNF repository site. Identification of the hydraulic property for the buffer medium, acquired through these experimental measurements is very rare and is considered to have high academic values. Experimental results from this study were used as input parameter values for the numerical modeling to simulate the long-term gas migration in the buffer zone and to evaluate the feasibility of the buffer material, controlling the radionuclide-gas migration in the SNF repository site.

The PRIDE scale mechanical decladder is decladding apparatus for separating and recovering fuel material and cladding hull by horizontally slitting rod-cut. In order to enhance mechanical decladdng efficiency, the main requirements were considered as follows. Decladding of the fuel rods may be performed by rotation of three circular cutting blades inserted among the rollers arranged at 120° portion. In a mechanical decladder, a slitting assembly as a unit for slitting the cladding tube may include cutting blades for slitting and rollers for guiding extrusion of the cladding tube. Rotation of the cutting blades may be caused by the fuel rods being extruded from a plurality of rollers. Slitting intervals of rod-cuts having different diameters may be controlled by adding or removing a spacing plate between the cutting blade and a ranch bolt for fixing the slitting blade to the slitting assembly. An extrusion velocity with respect to the fuel rods may be controlled by a hydraulic pressure applied to the fuel rods. A force for cutting the fuel rods may be adjusted by controlling steel plates. Forces applied to a plurality of rollers may be generated by the hydraulic cylinder. The hydraulic pressure may be controlled by hydraulic pressure controller. The PRIDE scale mechanical decladder mainly consists of auto feeding module, hydraulic cylinder module and blade module. A load cell was installed between the hydraulic cylinder and the extrusion pin to measure the decladding force and slitting velocity, and a data acquisition system capable of obtaining data by using the RSC 232 was constructed. Also, the control panel can control the forward and backward movement of the extrusion pin, the hydraulic flow rate, and the hydraulic velocity. In the mechanical decladding test, 40 pieces of simulated rod-cuts were loaded in two auto feeding basket and slit by utilizing the 3-CUT blade modules in the housing, and hulls and simulated pellets were collected in the collection container. As a result, 80 pieces of simulated rodcut (brass pellets + Zry4 tube) were slit continuously without any problem. About 35 min was required to slit 80 rod-cuts and average decladding force was 260 kg. The decladding force of the ceramic simulated rod-cuts (castable) requires 25 kg less force than the brass pellets. Therefore, it is estimated that the spent fuel rod-cut can be fully split into three pieces using the mechanical decladder.

Protaetia brevitarsis seulensis larvae from industrial insects are traditionally recognized as functional health foods in South Korea. We evaluated the immuno-modulatory effects of feeding beneficial microorganism (Bacillus velezensis TJS119) to P. brevitarsis larvae as a dietary source. In this study, we investigated the immune-enhancing activities of P. brevitarsis larvae hot-water extract (PLW) and PLW after treatment with B. velezensis TJS119 (PLWB) using the RAW 264.7 macrophage cell line. We examined the effects of PLWB on cell proliferation, cytokine production, and nitric oxide production in RAW264.7 cells. PLWB showed no cytotoxicity at concentrations ranging from 7.8 to 1,000 μg/mL in RAW264.7 cells. Treatment with PLWB increased the production of nitric oxide and pro-inflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β)] at doses of 62.5 to 1,000 μ g/mL in RAW264.7 cells. As a result, PLWB exhibited a stronger immune-enhancing effect compared to PLW. In conclusion, the results of this study offer experimental evidence to support the potential utilization of PLWB as an immunity-enhancing nutraceutical ingredient.

Pine Wilt Disease (PWD) is a disease causing mass deaths of pine trees in South Korea, and the dead trees serve as breeding grounds for insect vectors responsible for spreading the disease to other host trees. Because the PWD requires early monitoring to minimize its damage on domestic forestry, this study aims to develop a species distribution model for predicting the potential distribution of PWD by using artificial neural network (ANN) with time-series data. Among the architectures, the Convolutional Neural Network exhibited the highest performance, achieving a validation accuracy of 0.854 and a cross-entropy loss of 0.401, and the InceptionTime model emerged as the second-best performer. This study identified the best-performing ANN architecture for a spatiotemporal evaluation of PWD occurrence, emphasizing the importance for determining hyperparameters with ecological characteristics and data types to apply deep learning into SDMs.

2022년 10월부터 2023년 5월까지 친환경 딸기재배 농가에서 점박이응애는 칠레이리애응애와 사막이리응애, 진딧물은 콜레마니진디벌을 대상으로 하는 천적처리구와 유기농자재를 사용하는 관행방제구에서 천적의 해 충 밀도억제 효과를 조사하였다. 천적처리구에서 점박이응애 성충 밀도는 잎당 1.5마리 이하, 알은 4개 이하로 관리되었고, 사막이리응애는 잎당 최대 0.4마리까지 증가하여 점박이응애 밀도억제에 많은 영향을 미친것으로 보인다. 반면, 관행방제구에서 점박이응애 밀도는 천적 방사구에 비하여 오히려 많은 발생량을 보였지만, 3월 9일부터는 사막이리응애의 증가와 유기농자재의 효과로 점박이응애 밀도는 급격하게 감소하였다. 진딧물 천적 처리구에서 진딧물 밀도는 1월부터 발생하였으며 3월 상순에 잎당 0.3마리까지 증가하였으나 이후 감소하였고, 콜레마니진디벌은 진딧물 발생이 많지 않아 3.9마리/㎡(2회) 방사하는 데 그쳤다. 한편, 관행방제구 포장의 진딧 물은 유기농자재의 영향으로 거의 발생하지 않았다.