This study was carried out to investigate the growth inhibition of Propionibacterium acnes by mycelial culture broth of Paecilomyces japonica in the Mulberry leaf extract. The results are as following. The growth inhibition effect of P. japonica mycelial culture broth against P. acnes in various concentration of Mulberry leaf extract was the most effective in 3% Mulberry leaf extract. The inhibition effect of P. japonica mycelial culture broth against P. acnes in Mulberry leaf extract according to the culture time was the moust effective for 25 days cultivation. As the treating volume of the mycelial culture broth was increased, the growth inhibition effect against P. acnes was increased as well. The growth inhibition effect of mycelial culture broth against P. acnes according to the time of heat treatment was active by 45min at 100℃, while it was inactive at more than 60min. Taken together P. japonica mycelial culture in the Mulbarry leaf extract has a possibility to be an element of skincare cosmetics regulating the acnes. This means that the enzyme may be used for health-care such as thrombosis without any hamful behavour in the blood vessel.