Estimating compensation payment for environmental damages by chemical accidents has been controversial because accurate quantification of damages of chemical accident is so complicated and there is lack of scientific studies about impact of chemical accidents. In this study, for supporting chemical accidents compensation criteria, the 7 days acute and 28 days chronic toxicity test was conducted with 4 major chemical accident substances (Sulfuric acid, Nitric acid, Methanol and Methylethylketone) according to the OECD 232 guidelines. Methylethylketone was most toxic chemical followed by methanol, nitric acid and surfuric acid. Further study of comparison between pH and chemical effects was needed.