최근 주요 절화 수입량이 증가하면서 외래 병해충 유입률 또한 증가하고 있다. 농림축산검역본부 병해충정보 시스템(PIS)에 따르면 최근 10년간(2011-2020년) 수입된 절화류에서 검출된 총채벌레목은 총 43종 6,734건이다. 그중 가장 많이 검출된 총채벌레는 Frankliniella occidentalis (2,526건), Thrips tabaci (1,470건)로 전체 검출건수의 59% 이상 차지한다. 본 연구에서는 수입 절화류에서 검출률이 가장 높은 총채벌레 두 종의 미토콘드리아 DNA CO I 유전자를 이용하여 염기변이에 의한 국가별 다양성과 유전적 다양도 분석을 실시하였다. 네덜란드, 중국 등 7개국에서 검출한 Frankliniella occidentalis 184개 개체에서 9가지의 단상형(haplotype)이 관찰되었다. 또한 페루, 네덜란드 등 6개 국가에서 검출된 Thrips tabaci 80개 개체에서는 7가지 단상형이 보였다. 본 연구에서 검출 한 mtDNA내 염기서열 다형성은 총채벌레 집단의 국가적 변이성 추정 및 연관성 분석에 기초자료로 활용할 것으 로 기대한다.

코드린나방은 사과, 배, 복숭아 등의 과실류를 비롯한 다양한 작물의 해충으로써, 국내에서는 검역 대상으로 금지해충에 속해있다. 코드린나방이 속한 잎말이나방의 유충은 과실 속으로 파고 들어가 살기 때문에 살충제로 방제하기가 어려운 해충이다. 따라서 철저한 수입식물 검역과 예찰을 통해 국내로의 유입을 막는 것이 최선이다. 현재 잎말이나방류에 대한 예찰용 페로몬트랩이 사용되고 있지만 개별 종에 대한 표적 예찰은 이뤄지지 않고 있다. 본 연구에서는 코드린나방의 성페로몬을 이용한 유인제 개발을 위한 기초연구로써, 현재 상용화되어 미국, 유럽, 중국 등에서 사용하고 있는 다양한 유인제의 조성성분을 분석하였다. 전세계적에서 대표적으로 사용되는 4종류의 코드린나방 루어를 GC-MS/MS를 이용하여 물질의 종류와 구성비율을 분석하였다. 본 연구의 결과는 향후 코드린나방의 최적 예찰을 위한 성페로몬 유인제 선발의 기초자료로 활용될 예정이다.

The striped fruit fly (SFF), Zeugodacus scutellata, is an agricultural pest species with a strong and rapid reproductive ability that can cause significant harm. To control the population of these kind of pests, the sterile insect technique (SIT) is being used as one of the effective methods. SIT involves the introduction of sexually transmitted factors that reduce the reproductive capacity of males. This study shows that knocking down the testis-specific serine/threonine protein kinase 1 (Zs-Tssk1) gene alters male fertility and male-initiated types of communication. Since Zs-Tssk1 influences the physiology of the testes, spermatogenesis is also affected, which in turn alters the lifespan of Zs-Tssk1 knock down group in comparison with the control. Based on these results, Zs-Tssk1 may be crucial in reproductive function, and its down-regulation may be helpful in controlling SFF through SIT.

호박꽃과실파리(Z. scutellata)는 박과작물에 피해를 주는 중요한 해충이다. 현재 큐루어(Cuelure)는 국내에서 검역 대상 해충으로 규정된 오이과실파리, 타우과실파리, 및 퀸슬랜드과실파리와 더불어 호박꽃과실파리 수컷 성충의 예찰에 다양한 제형으로 활용되고 있다. 본 연구는 액 체, 고체, 및 왁스제형의 큐루어에 대한 호박꽃과실파리 수컷 성충의 야외 유인효과를 검증하기 위한 것으로 2021년 4월부터 10월까지 제주 2개 지역과 경북 1개 조사에서 2주 간격으로 모니터링하였다. 호박꽃과실파리의 발생밀도가 높은 제주지역에서는 7월부터 8월에 액체와 왁스제형의 큐루어에 호박꽃과실파리가 더 많이 유인되어 통계적인 유의차가 있었으나, 호박꽃과실파리의 발생밀도가 낮은 경북지역에서는 제형별 유인력 에 차이가 없었다. 야외조사 결과를 바탕으로 액체와 왁스제형의 큐루어가 호박꽃과실파리 수컷 성충 예찰에 권장되며, 이 두 제형의 큐루어에 유 인되는 검역 대상 과실파리류의 국내 예찰에 효과적일 수 있다.

Acute and chronic toxicities of methyl ethyl ketone and methanol were investigated on Paronychiurus kimi (Collembola), for evaluating the potential effects of accidental exposures of these chemical substances on the terrestrial environments. This study was undertaken to establish a toxicity database for these chemical substances, which was required for the preparation of the response compensation and liability act for agricultural production and environmental damage. The 7-d acute toxicity and 28-d chronic toxicity were conducted using the OECD artificial soil spiked with varying, serially diluted concentrations of methyl ethyl ketone and methanol. Mortality was recorded after 7-d and 28-d of exposures, and the number of juveniles were determined after 28-d of exposure in the chronic toxicity test. In both assessments, methanol was more toxic than methyl ethyl ketone in terms of mortality (LC50) and reproduction (EC50). The 7-d LC50 of methyl ethyl ketone and methanol were 762 and 2378 mg kg-1 soil dry wt., respectively, and the 28-d LC50s were 6063 and 1857 mg kg-1 soil dry wt., respectively. The 28-d EC50 of methyl ethyl ketone and methanol were 265 and 602 mg kg-1 soil dry wt., respectively. Comparison of results obtained in this study with literature data revealed that P. kimi was more sensitive to methanol than other soil invertebrates. However, given the high volatility of the chemicals tested in this study, further studies are necessary to improve the current test guideline, or to develop new test guidelines for an accurate assessment of chemicals that require toxicity databases for chemical accidents.

Estimating compensation payment for environmental damages by chemical accidents has been controversial because accurate quantification of damages of chemical accident is so complicated and there is lack of scientific studies about impact of chemical accidents. In this study, for supporting chemical accidents compensation criteria, the 7 days acute and 28 days chronic toxicity test was conducted with 4 major chemical accident substances (Sulfuric acid, Nitric acid, Methanol and Methylethylketone) according to the OECD 232 guidelines. Methylethylketone was most toxic chemical followed by methanol, nitric acid and surfuric acid. Further study of comparison between pH and chemical effects was needed.

Despite the importance of behavioral ecotoxicological tests, few researches have been conducted to assess the quality of freshwater using behavior of test species, in part due to lack of standardized test method for quantifying the response. In this study, we developed a new test methods using non-biting midge larva’s burrowing behavior to assess field water quality and a new index, AUC (area under curve), to compare burrowing behavior quantitatively. Four kinds of field samples (residential, natural, agricultural and industrial areas) were tested using the method to validate our methodology and index. There were not significant differences in 96 h mortality and heavy metal concentration between samples, but AUC from the industry sample was significantly decreased.

Much of the data used in the analysis of environmental ecological data is being obtained over time. If the number of time points is small, the data will not be given enough information, so repeated measurements or multiple survey points data should be used to perform a comprehensive analysis. The method used for that case is longitudinal data analysis or mixed model analysis. However, if the amount of information is sufficient due to the large number of time points, repetitive data are not needed and these data are analyzed using time series analysis technique. In particular, with a large number of data points in the current situation, when we want to predict how each variable affects each other, or what trends will be expected in the future, we should analyze the data using time series analysis techniques. In this study, we introduce univariate time series analysis, intervention time series model, transfer function model, and multivariate time series model and review research papers studied in Korea. We also introduce an error correction model, which can be used to analyze environmental ecological data.

We developed a new ecotoxicological test method using native test species, eggs of Ephemera orientalis, and five kinds of industrial wastewater were tested to validate the test method. The water samples were collected in Jun 2006 from the following industries: pesticide, metal plating, PCB, leather1, and leather2. Wastewater and effluent were diluted by distilled water, respectively, to prepare various concentrations, 100, 50, 25, 12.5, 6.3, 3.1, and 0%. For the egg bioassay, 20 freshly laid eggs (<24 h old) were exposed to test solutions in a Petri dish (52×12 mm) at 20℃ with photoperiod of 16 h light and 8 h dark for 14 days. The median egg hatching concentrations (EHC50) were estimated using Probit analysis. All EHC50s of wastewater were less than 3.1%, which meant very high ecotoxicity except for the wastewater of PCB industry having 6.1% of EHC50. Among the effluents, the least toxic effluent was from pesticide industry having 58% of EHC50, while the effluent of leather2 was the most toxic having 7.3% of EHC50.

Global warming can seriously influence on the interaction between pest and natural enemy in the agroecosystem due to the differences in optimal temperature ranges. Two aphid-ladybug systems, Myzus persicae-Coccinella septempunctata (M-C) and Aphis gossypii-Coccinella septempunctata (A-C) in the pepper crop were simulated, respectively under four different temperature scenarios including crop development over 244 days with the assumption that the average temperature is higher by 1, 3, and 5 °C than that in Seoul in 2000. Temperature-dependent functions for each aphid-ladybug system were embedded in Rosenzweig-Macathur predator-prey model to explore their population dynamics, and then Dynamic Index was used to quantify the strengths of species interactions. The result shows that the predator-prey population cycles as well as species interactions are getting shorter and stronger in both systems as temperature increased. Especially, the excessively high temperature scenario in Aphis gossypii-Coccinella septempunctata system could result in the extreme species interaction. Therefore, the increasing temperature can facilitate the effectiveness of biological control because of growing crop plant development and much stronger species interaction, although there are increases of the frequency of pest occurrences.

기후변화에 따른 해충개체군 증감모형은 해충방제를 위 한 초발생예찰과 연속적 해충변동 양상의 파악에 매우 중 요하다. 이러한 예측은 농약사용의 효율성을 높이고, 환경 에 적은 영향을 줄 수 있으므로, 현대 해충방제전략의 화두 로 볼 수 있다. 본 연구는 온도변화에 따른 해충의 농약효과 에 따른 사충률의 변화를 개체군 모형과 결합시켜 모의했다. 감수성 점박이응애를 강낭콩을 기주로 20, 25, 30, 35℃에서 Acrinathrin-Spiromesifen 혼합제와 Azocyclotin 유기주석계 농약에 노출시켰다. 생물검정 결과 점박이응애의 사충률은 온도와 농약의 종류에 따라 유의한 차이가 발생했다. 점박이 응애의 개체군 밀도변동 모의는 DYMEX를 이용했으며, 모 의결과 농약의 종류별로 기후변화에 따른 초기방제 시기와 방제횟수에 차이가 나타날 것으로 예측됐다. 본 연구결과는 미래의 기후변화에 대응한 해충방제 전략과 농약 선발에 있 어 중요한 시사점을 제공할 것으로 사료된다.

Araneae species are predators in natural ecosystem interact with various prey species. These linkage can be affected under climate change because species react not just individually but systematically. We focused on potential impact of climate change in Araneae fauna in national scale. In this study, potential species richness of Araneae in South Korea was predicted with MaxEnt (Maximum Entropy) model. Korea Forest Research Institute conducted national scale research of wandering arthropods. They monitored in uniformly set 366 points, and the data contain exact GPS points of study sites. Occurrence data were extracted from Prediction of Distribution and Abundance of Forest Spiders According to Climate Scenario (Korea Forest Research Institute, 2013). With the report, dominant 21 Araneae species that appeared more than 10% study sites were selected to estimate species richness. Training climate data were prepared from observation source of Korea Meteorological Administration. RCP 8.5 scenario data which represent future (2050, 2070) climate condition were downloaded from WORLDCLIM web site. In MaxEnt simulation, occurrence data for 21 species and 19 bioclimatic variables were used. Because the model outputs are expressed in index, the minimum training presence threshold rule was applied to distinguish presence/absence of each 21 species distribution model. We overlaid whole 21 thresholded output to get species richness map. The fluctuation between current and future species richness was calculated to observe changing trend in national scale. The results of Araneae fauna tends to move higher altitude and latitude. Species richness of lowlands is predicted to be diminished, but higher mountains are expected to be more suitable for many spider species. In some South Western coastal areas showed reduced richness in 2050 but will recover in 2070.

Behavioral response is commonly affected by heavy metal toxicity, and behavioral reaction can be measured as sensitive endpoint for sublethal toxicity test and obtained easily and quickly. Also behavioral endpoints may serve as an insightful evaluation tool of the ecological effects of toxic chemicals. In this study, Heterocypris incongruens (Crustacea: Ostracoda) was selected, which is usually used as an indicater species for water quality. H. incongruens was exposed to copper and arsenic for 5 minute and total distance, velocity, turn angle, and angle velocity were measured for 30 minute using video analysis system in laboratory condition. Each endpoints reflected the effects of copper and Arsenic toxicity appropriately. These endpoint have possibility that can be used to identify characteristic behavioral responses to metal toxicity.

Thc climate change has the potential to significantly modify the actual distribution of insect pest with unknown consequences on agricultural systems and management strategies. In this study, Thrips palmi Karny was selected to predict distribution under climate change. T. palmi was introduced and first recorded in 1993 in Korea, and has become a serious pest of vegetable and ornamental crops. The MaxEnt was applied to T. palmi to predict its potential geographic distribution in Korea and Japan under the RCP 8.5 climate changing scenario. The MaxEnt software package is one of the most popular tools for species distribution and environmental niche modeling. The habitat prediction model of T. palmi in Korea was validated by the distribution of T. palmi in Japan. Based on the MaxEnt modeling, T. palmi would expand their potential distribution to whole Korean peninsula except the alpine region in Gangwon-do and Yanggang-do and Hamgyeongbuk-do in 2070s. Therefore, the monitoring system and management strategy for T. palmi should be reconsidered and re-evaluated.