Seaweed-derived foods have long been popular in Korea because of their high content of nutrients that are beneficial to the human body. Recently, Korean seaweeds have been used as raw materials to produce new natural products with health benefits. Herein, we compared the antioxidant activity of 16 Korean seaweed extracts to explore their potential utility as health foods. The total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of seaweed extracts were determined. We also investigated their ability to protect human diploid fibroblast (HDF) cells against hydrogen peroxide. The results showed that seaweed extracts at a concentration of 100 g/mL did not cause any cell toxicity. Sargassum thunbergii (Jichung-i) had the highest TPC and radical scavenging effects, followed by Porphyra tenera (Gim), Silvetia siliquosa (Tteumbugi), and Sargassum fusiforme (Tot). Hydrogen peroxide increased the production of intracellular reactive oxygen species, while P. tenera (Gim), Saccharina japonica (Dasima), and S. thunbergii (Jichung-i) extracts significantly decreased it. The effect was highest in the S. thunbergii (Jichung-i)-treated HDF cells. These findings indicate that S. thunbergii (Jichung-i) shows promise as a potential antioxidant raw material.

This study investigated the quality characteristics and antioxidant effects of rice cookies containing different percentage of freeze-dried plum powder (3%, 5%, 6%, and 12%). The density of the dough and the thickness of the rice cookies from the control group and the additive group were not significantly different. The pH value of the dough, its moisture content and spread factor, as well as the Hunter’s L and b values of the rice cookies significantly decreased as the amount of dried plum powder added increased. However, the sweetness, hardness, thickness, Hunter’s a value, total polyphenol, total flavonoid and anthocyanin contents, DPPH and ABTS free radials scavenging activity, and ferric reducing antioxidant power of the rice cookies significantly increased as the amount of dried plum powder added increased. Overall, the results of this study showed that adding dried plum power enhanced the antioxidant activities and quality characteristics of rice cookies.

In this study, we analyzed the antifungal activities of five essential oils (clove, rosemary, thyme, basil, and oregano) against three fungi (Penicillium oxalicum, Penicillium commune, and Cladosporium cladosporioides) isolated from farm-type fermented meat products Though their antifungal activities differed for each fungus, thyme had the greatest effect. Notably, C. cladosporioides showed the highest sensitivity to essential oils, and growth inhibitory effects were greater than for the other two strains. Additionally, ABTS, DPPH, and FRAP analysis confirmed that the five essential oils studied had antioxidant activity. ABTS analysis showed that clove (75%) and oregano (75%) oils had the highest antioxidant activities (both 93.7%). DPPH analysis showed that clove (75%) and rosemary (75%) oils had significantly greater antioxidant activities (both 93.8%) than thyme, basil, or oregano oils. FRAP results indicated that clove and basil oils were the strongest reductants. Comprehensive comparative analysis indicated that clove oil had more antioxidant activity than the other four essential oils. Overall, the study shows that the excellent antifungal properties of clove oil could be harnessed to produce safe fermented meat products by preventing rancidity and mold contamination.

본 연구에서는 비건 인증(Vegan) 화장품 원료 7종 혼합추출물의 항산화능 및 미백효과를 확인 하였다. 라디컬 소거능으로서 DPPH, ABTS, FRAP 실험, 항산화 물질 측정으로서 폴리페놀과 플라보노이 드 함량 측정을 실시하였다. DPPH 실험에서는 105.74 mg ascorbic acid / g의 항산화능을 나타내었으며, ABTS 실험에서는 85.31 mg ascorbic acid / g의 항산화능을 나타내었다. FRAP에서는 7종 혼합추출물의 1 mg이 ascorbic acid 198.01±5.50 μg의 환원력을 보였다. 폴리페놀 함량은 30.19±0.75 mg/g으로 나타 났으며, 플라보노이드 함량은 9.12±0.36 mg/g으로 나타났다. 한편 세포실험에서는 세포 독성과 미백활성 을 알아보았다. 세포독성의 경우 20% 이하의 세포 독성을 보였으며, 100 μg/mL 농도에서 34.70±2.97% 의 멜라닌 생성 억제능을 보여 7종 혼합추출물의 기능성 화장품 원료로서의 가능성을 확인하였다.

Yogurt is mostly consumed dairy food and considered as a nutritious food because of its probiotics such as lactic acid bacteria (LAB). LABs are known to possess antioxidant, anticarcinogenic and antimicrobial effects. Corni fructus has healthy and pharmacological effects such as the inhibition of apidogenesis and angiopoiesis. Thus, the purpose of this study was to evaluate quality characteristics and antioxidant activity of yogurt supplemented with water and ethanol extracts of corni fructus. Yogurt was manufactured with addition of 0.1%-0.2% (w/v) water or ethanol corni fructus extracts, respectively. For quality characteristics, pH, chromaticity, viscosity and syneresis were determined during refrigeration storage (1, 7, 14, and 21 day). For antioxidant activities, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activities were examined during storage. The pH showed no significant difference between groups but showed decreasing pattern for all groups during storage period. In chromaticity, redness increased, and lightness decreased as the amount of corni furctus extracts increases. Yogurt with corni furctus ethanol extracts showed more color changes than yogurt with corni furctus water extracts. Viscosity increased in corni furctus extract added groups, compared to control. Syneresis was approximately 2-fold higher in corni fructus extract added groups, while there were no significant changes between control and extract added groups. Both DPPH and ABTS radical scavenging activities were significantly higher than control group in corni fructus extract added groups, compared to control. In conclusion, addition of corni fructus extracts in yogurt improved quality characteristics during storage and antioxidant properties.

This study examined the bioactive compound content and the antioxidant activities of bitter melon (Momordica charantia Linn.) leaves. The content of vitamin C, beta-carotene, and total carotenoids was 69.77, 45.68, and 65.08 mg/100 g, respectively. To investigate the antioxidant capacity, bitter melon leaves were extracted using various concentrations of ethanol (60, 80, or 100%). Highest content of total polyphenols (18.07 mg gallic acid equivalent/g) and flavonoids (4.53 mg cathechin equivalent/g) was found in the 100% ethanolic extract of the leaves (E100). Also, the E100 extract showed the highest levels of 2,2'azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging activities. Reducing power was also the highest (39.21 mg Trolox equivalent/g) in E100 extract. The E100 extract effectively inhibited lipid peroxidation by 91.45% compared to the control group. Also, the E100 extract showed a cytoprotective effect against oxidative stress in HepG2 cells and decreased the generation of intracellular reactive oxygen species. These results suggest that bitter melon leaves could be regarded as a potential source of natural antioxidants.

균근성 버섯이란 외생균근(ectomycorrhizae)이 수목의 뿌리에 침입하여 균투 (fungal sheath), 세포 간 균사(hyphae)와 하티그망(hartig net)을 만들어 기주 식물과 공생하며 사는 절대생물영양성(obligate biotroph) 고등균류임 균근성 버섯을 만드는 버섯류로는 주로 담자균류이며 대부분의 겉씨식물과 일 부의 속씨식물과 공생하는 것으로 알려짐 국내 야생버섯 중 균근성 버섯은 약 35%를 차지하며 국내에서 잘 알려진 균근 성 버섯으로는 송이, 능이, 싸리버섯, 꾀꼬리버섯, 달걀버섯 등이 있음 본 연구에서는 국내 자생하는 균근성 버섯의 생리활성 분석을 통하여 우수한 자원을 발굴함과 동시에 토종 버섯자원을 이용한 새로운 천연물 유래 생리활 성 물질로 활용하기 위한 기초자료를 제시하고자 함

The extracts of four species of ectomycorrhizal mushrooms—Cantharellus cinnabarinus (OK1247), Lactarius parallelus (OK1264), Tricholoma matsutake (OK1282), and Ramaria botrytis (OK1283)—were prepared to determine their antioxidant activities and nutritional properties. R. botrytis extract displayed the highest 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (33.8%), ferric reducing antioxidant power (0.38), reducing power (0.35), total polyphenol (13.83 mg gallic acid equivalent/g), and flavonoid contents (2.56 mg quercetin equivalent/g). L. parallelus extract displayed the highest nitrite scavenging activity. Analysis of amino acid contents revealed that C. cinnabarinus extract had the highest total amino acid (1,046.1 mg/kg) and essential amino acid (404.9 mg/kg) contents, while R. botrytis extract had the lowest total amino acid (708.3 mg/kg) and essential amino acid (247.3 mg/kg) contents. Among the amino acid components detected in the four ectomycorrhizal mushrooms, cysteine was the most abundant, accounting for 14.3~20.7%, followed by phenylalanine, which accounted for 9.5 ~13.4% of all amino acids. In summary, the antioxidant activities were the highest in R. botrytis extract, and the amino acid content was the highest in C. cinnabarinus extract, among the four ectomycorrhizal mushrooms.

본 연구는 귀리의 겨, 겉껍질 및 잎을 에탄올로 추출한 후 항산화 및 항균활성을 조사하였으며, 추출물을 유화형 돈육 소시지에 적용하였을 때 품질 및 저장성에 미치는 영향을 알아보기 위하여 수행하였다. 귀리의 잎 추출물(OLE)의 총 페놀함량(145.99ug CAE/mg), ABTS 라디칼 소거 활성(35.07%) 및 FRAP 활성(32.77ug Fe2+/mg)은 다른 부위에 비해 유의적으로 높게 나타났다(p<0.05). 항균 활성 측정결과 모든 부위의 추출물에서 Staphylococcus aureus, Streptococcus pneumonia과 Escherichia coli에 대해서 활성을 보이지 않았으나, OLE 및 귀리 겨 추출물은 Klebsiella pneumonia에 대해서는 120μg/disk이상에서 활성을 보였으며, OLE가 귀리 겨 추출물보다 동일 농도에서 유의적으로 높은 활성을 나타내었다(p<0.05). OLE를 유화형 돈육 소시지에 첨가하였을 때, 0.025% 첨가구에서 pH, 조직감 및 관능적 특성에 유의적인 영향을 미치지 않았다. 하지만 저장 14일차에 모든 첨가구에서 지방산화(TBARS), volatile basic nitrogen (VBN), 총균수가 대조구보다 유의적으로 낮게 측정되었다(p<0.05). 본 실험결과 OLE는 높은 항산화 활성을 보였으며, 유화형 돈육 소시지 제품에 적용하였을 때 지방산화를 지연시켰고, 총균수의 저하를 가져왔기 때문에 천연 보존제로서 좋은 첨가제로 사료된다.

In this paper, quality properties and antioxidant capacity of cooked black carrot has been studied. Five different cooking methods have been applied: microwave (45 sec), boiling (100℃, 14 min), steaming (100℃, 20 min), sous vide (75℃, 80 min), sous-vide (95℃, 30 min). The color value was slightly different (p<0.05), the boiling method was the highest in L-value and Sous vide 75℃ method was the lowest. The steaming method indicated the highest a-value at 5.50. The b-value was significantly different between the samples (p<0.05). The microwave method was the highest at 3.49 while the sous vide 95℃ method was the lowest at －0.34. No significant difference was observed between the samples when only the moisture content results were considered. The highest pH was observed in samples cooked using the boiling method. The softening (%) was higher when the cooking water was in contact with samples and it was dependent on the temperatures. In addition, this study shows that not only temperature but also cooking time and cooking media are very crucial in the preparation and cooking of black carrot to prevent the loss of the antioxidant compound. In conclusion, the comparison of the quality characteristics and antioxidant properties of black carrots indicated that the most appropriate cooking method is sous-vide method.

본 연구는 금잔화를 꽃차로 이용하기 위해 열풍 건조 처리 시간에 따른 항산화 물질함량 및 항산화 활성을 분석하고, 최적 처리 시간을 구명하기 위해 수행하였다. 식용꽃 금잔화를 60℃에서 5시간, 10시간, 15시간 열풍건조 한 뒤 Hunter value를 측정하고 95% 에탄올을 용매로 하여 추출하였다. 항산화 물질인 총 폴리페놀, 총 플라보노이드, 총 카로티노이드 함량과 DPPH radical 소거 활성을 통한 항산화 활성, 이를 통한 항산화 물질 함량과 항산화 활성 간의 상관관계를 분석하였다. 처리시간이 증가함에 따라 Hunter value L 값과 +b값이 모두 감소하는 경향을 보였으며, 15시간 처리구에서 다소 많이 감소하였다. 또한, 항산화 물질 함량을 조사한 결과, 총 폴리페놀 함량은 처리 시간에 따른 차이는 없었으나 총 플라보노이드 함량과 총 카로티노이드 함량은 5시간과 10시간 처리시 함량이 다소 높았으며, 처리 시간이 증가함에 따라 감소하여 15시간 처리시 대조구와 차이가 없는 것으로 조사되었다. DPPH radical 소거 활성 분석 결과, 5시간과 10시간 처리시 대조구보다 활성이 높았으며, 총 카로티노이드가 DPPH radical 소거 활성 간의 상관관계가 높은 것으로 나타났다(p≤0.01, r=0.610). 따라서, 열풍건조한 금잔화는 총 카로티노이드가 항산화 활성에 기여하며, 5시간 및 10시간 열풍건조 처리가 금잔화의 항산화 물질 함량 및 활성 증가에 효과적인 것으로 판단되나 식물의 열처리 가공에 있어 오랜 시간은 내부 조직 및 항산화 물질 파괴에 영향을 미칠 수 있기 때문에 금잔화 열풍 건조 처리 시 5시간이 효과적일 것으로 판단된다.

This study evaluated the quality characteristics of rice cookies prepared with different amounts (0, 3, 6, 9%) of hibiscus powder. The pH of cookie dough decreased as the amount of hibiscus powder increased. Additionally, the moisture content and spread factor were higher in groups containing hibiscus powder than in the control groups. Furthermore, the L-value decreased with increasing hibiscus powder, while the a-value increased. The incorporation of hibiscus powder into cookies decreased hardness. Additionally, consumer acceptance testing revealed that the addition of up to 6% hibiscus powder was desirable in terms of overall acceptability. The total phenol content of the control groups was 12.32 mg GAE/100 g, while the levels in the groups containing hibiscus powder ranged from 23.32 to 59.86 mg GAE/100 g. Finally, DPPH and ABTS radical scavenging activity increased with increasing hibiscus powder level. Taken together, the results of this study indicate that amending cookies with 6% hibiscus powder can improve antioxidant activities without affecting sensory quality.

서리태는 검은콩의 한 종류로 활성산소를 제거하는 항산화 효과가 있다. 주요 유효 성분은 isoflvaone이며 대표적인 isoflavone은 genistein, daidzein 등으로 12종의 isoflavone이 존재하며 화학적 구조에 따라서 배당체(glycoside)와 비배당체(aglycone)로 구분된다. 팽화는 짧은 시간에 시료를 고온고압 처리함으로써 식품의 물리화학적 성질을 변화시키는 기술로 이 연구에 사용된 pressure-drop법은 팽화기 내외부의 압력 차이를 이용해 물질 내부의 수증기압에 의한 수증기의 외부 방출로 인해 부피 팽창을 유도하는 방법이다. 본 연구는 서리태의 유효성분인 isoflavone과 항산화능이 팽화에 따라 변화되는 현상을 확인하기 위하여 다양한 압력 조건 하에서 서리태의 팽화를 진행하고 이들의 isoflavone 및 항산화능의 변화를 연구하였다. 팽화압력 조건은 490, 588, 686, 784 kPa로 진행되었으며 팽화하지 않은 sample을 control로 비교하였다. Morphology를 비교한 결과 팽화 압력이 증가할수록 껍질이 벗겨지고 표면에 균열이 생기는 현상이 심화되었다. 또한 팽화로 인해 크기가 약간 증가한 것을 확인할 수 있었지만 팽화 압력에 따른 유의차는 보이지 않았다. 팽화 후 각 sample은 70% 에탄올로 추출 후 농축을 거쳐 증류수에 녹여 사용하였다. 추출 수율은 팽화 처리에 의해 유의적인 차이를 보이지 않았다. Isoflavone 함량을 비교한 결과 팽화한 시료의 경우 control sample에 존재하던 malonyl daidzin, malonyl glycitin, malonyl genistin이 사라졌다. Acetyl daidzin, acetyl genistin, Daidzin, genistin은 팽화 압력이 증가함에 따라 증가하다가 784kPa에서 급격히 감소하였다. Glycitin, daidzein의 경우 팽화 압력에 따라 일정한 경향을 보이지 않았으며 genistein은 팽화 압력에 따라 증가하는 경향을 나타내었다. Acetyl glycitin, glycitein은 팽화와 상관없이 모두 검출되지 않았다. 팽화한 서리태의 DPPH radical, ABTS radical 소거능과 total phenolic contents는 control에 비해 증가하였고 팽화 압력에 따라 대체로 증가하는 경향을 나타낸 반면, total flavonoid contents는 일관성 있는 경향을 보이지는 않았지만 모든 팽화 조건에서 control에 비해 증가한 결과를 나타내었다. 본 연구를 통하여 팽화 처리가 서리태의 유효 성분 증가 및 변환에 긍정적인 효과를 나타내었고, 항산화능 증가를 위한 유효한 가공 처리가 될 수 있으며, isoflavone 조성을 효과적으로 변환할 수 있는 적절한 가공 방법임을 확인할 수 있었다.