Captive breeding and reintroduction are crucial strategies for conserving endangered species populations. However, fish raised in predator-free environments, show a lack of recognition of predationrelated stimuli such as chemical and visual signals. It is critical to recognize chemical signals from injured conspecifics, also known as alarm signals, and the order or shape of predators to indicate the spread of predation risk in the habitat. We conducted a laboratory experiment to determine and adjust the optimal exposure period to induce appropriate anti-predator behavior response to different types of stimuli (Chemical, Visual and Chemical+Visual) for the endangered species Microphysogobio rapidus. Our results demonstrate that predator avoidance behavior varies depending on the types of stimuli and the duration of predation risk exposure. First, the results showed captive-breed M. rapidus show lack of response against conspecific alarm signal (Chemical cue) before the predation risk exposure period and tend to increase response over predation risk exposure time. Second, response to predator (visual cue) tend to peak at 48 hours cumulative exposure, but show dramatic decrease after 72 hours cumulative exposure. Finally, response to the mixed cue (Chemical+visual) tend to peak prior to the predation risk exposure period and show reduced response during subsequent exposure periods. This experiment confirms the lack of responsiveness to conspecific alarm signals in captive-bred M. rapidus and the need for an optimal nature behavior enhancement program prior to release of endangered species. Furthermore, responsiveness to predator visual signal peak at 48 hours cumulative exposure, suggest an optimal predation risk exposure period of up to 48 hours. Key words: predator cognition, captive breeding, chemical signal, visual signal, endangered

시설재배지 고추를 가해하는 꽃노랑총채벌레(Frankliniella occidentalis)를 대상으로 미끌애꽃노린재(Orius laevigatus)를 이용한 생물적 방 제가 검토되고 있다. 그러나 대상 해충의 빠른 집단 성장은 화학 살충제의 투입이 때에 따라 요구된다. 본 연구는 화학 살충제와 천적의 이상적 종합 방제를 구현하기 위한 목적으로 선택성이 높은 살충제 선발 및 이들 살충제 처리 이후 미끌애꽃노린재의 안전한 재투입 시기를 결정하기 위해 수행 되었다. 첫째로 꽃노랑총채벌레에 방제 효과가 높은 상용 살충제가 선발되었다. 총 17종류의 상용 살충제 가운데 5종류(pyriproxyfen+ spinetoram, abamectin, spinosad, acetamiprid, chlorpyrifos) 주성분을 갖는 상용 살충제가 꽃노랑총채벌레에 우수한 방제효과를 주는 약제로 선발되었다. 이들 5종류의 살충제에 대해서 미끌애꽃노린재의 감수성 반응은 꽃노랑총채벌레와 상이하였다. 특별히 아바멕틴과 스피네토람이 유 기인계 또는 네오니코티노이드에 비해 상대적으로 낮은 독성을 보였다. 이들 5종류의 살충제 처리 이후 잔류 독성을 미끌애꽃노린재를 이용하여 생물검정한 결과 유기인계 및 네오니코티노이드 약제는 비교적 오랜 기간 독성을 유지하지만, 아바멕틴과 스피네토람 약제의 경우 3일 이후에는 대상 천적에 피해를 주지 않는 것으로 나타났다. 이러한 잔류독성결과는 LC-MS/MS를 이용한 농약 잔류량 화학분석을 통해 뒷받침되었다. 이상 의 결과는 높은 밀도로 증가한 꽃노랑총채벌레에 대해서 이 해충에 살충성이 높은 아바멕틴 또는 스피네토람의 약제를 살포하고 이후 3일 지나 미 끌애꽃노린재의 투입을 통해 대상 해충의 평균 밀도를 경제적피해수준 이하로 유지할 수 있다는 종합방제 기술을 제시하고 있다.

This study was conducted aiming to figure out two things. First, knowledge of the change in spatial distribution of Tetranychus urticae depending on how to control it (using pesticide or natural enemy). Second, spatial association of T. urticae and Phytoseiulus persimilis in biocontrol plot (B.P). The data was analyzed by spatial analysis by distance indices (SADIE) using global aggregation index, Ia . Ia values were 0.77-1.37 in conventional plot (C.P) and 0.88-1.68 in B.P, respectively. However, the fluctuation level of Ia in B.P was higher than C.P. Therefore, the results indicated that there was a clear spatial pattern change in B.P, i.e. prey’s spatial distribution is affected by natural enemy. And spatial association analysis showed that T. urticae and P. persimilis have positively associated. It means that T. urticae is relatively low mobile prey, and P. persimilis is relatively high mobile predator.

Naturally occurring plant toxins, such as benzoates, have been shown to have insecticidal effects on some pest insects. In this study three commercially available benzoates, methyl benzoate (MB), ethyl benzoate (EB), and vinyl benzoate (VB), were assessed for their contact toxicity against Aphis gossypii Glover and its lacewing predator Chrysoperla carnea Stephens. Toxicity of 1% MB, EB, and VB showed 100%, 85% and 60% mortality of A. gossypii at 24 h. In addition, a mixture of MB and EB showed higher synergistic effect than mixtures of MB+VB or EB+VB against A. gossypii. Toxicity at 1% concentration of three benzoates against A. gossypii showed lower toxicity against the larvae of C. carnea after 72 h of exposure than A. gossypii. Our result suggest that benzoates have high potential as environmentally safe biopesticides for A. gossypii control.

Recently, it has been demonstrated in several predator-prey interactions that predators influence prey population dynamics by inducing behavioral changes in prey as well as by feeding on prey. Accumulating evidence supports that prey change habit use patterns and activity levels in repose to predation risks. In the studies reported here, we examined the resource use patterns by adult whitefly, Bemisia argentifolii (Hemiptera: Aleyrodidae) in response to its natural enemies. The first study examined whether, in the context of a trap crop system, differential predation risks among plants influence host choice patterns of adult whiteflies. We investigated whether whiteflies avoid natural enemies inhabiting a cash crop (poinsettia) and whether this behavior can be used to increase the movement of whiteflies to a trap crop (cucumber). Three natural enemies were tested: two predators, Amblyseius swirskii and Delphastus catalinae and a parasitoid Encarsia formosa. The presence of D. catalinae on cash crop induced significantly more whiteflies to disperse to predator-free trap crop, compared with cash crop with no predator. A. swirskii and E. formosa did not result in a significant increase. The second study examined habitat choice patterns by adult whiteflies in response to D. catalinae at different spatial scales. When female whiteflies were confined in small leaf-disc arenas, whiteflies significantly delayed settling on leaf-discs with predators compared those with no predators. The presence of D. catalinae altered the vertical distribution of whiteflies on cucumber plants. Whiteflies moved upward faster over time within the plant canopy when predator were present at lower canopy compared with plants with no predator. However, D. catalinae did not result in elevated between-plant movement of whiteflies in the greenhouse, compared with that with no predator. Predator avoidance behavior by adult whiteflies should be considered in the development of biological control programs.

Dynamics of predator-prey systems are strongly affected by the strategic behavior of both predator and prey. Thus, understanding the relationship between the strategic behavior and the species survival is necessary to comprehend the system resilience and stability. In the present study, we constructed a spatially explicit lattice model to simulate integrative predator (wolf)-prey (two rabbit species)-plant relationships. Wolves have only the hunting strategy, while rabbits have the hunting-escaping strategy. When a rabbit simultaneously encounters its predator (wolves) and prey (plant), either hunting or escaping should take priority. Hunting priority is referred to as hunting preferred strategy (HPS), while escape priority is referred to as escape preferred strategy (EPS). These strategies are associated with some degree of willingness to either hunt (H) or escape (E). One rabbit species takes HPS (HPS-rabbit) and the other rabbit species takes EPS (EPS-rabbit). We investigated the changes in predicted population density for wolves, rabbits, and plant with changes in the value of H and E. Simulation results indicated that EPS-rabbit had a greater chance for survival than HPS-rabbit regardless of the initial density of EPS-rabbit, and the chance was optimized at the appropriate values of E and H. In addition, we briefly discussed the development of our model as a tool for understanding behavioral strategies in specific predatorprey interactions.

본 실험은 오이재배시 사용되는 살충제 47종이 진딧물 포식성 천적인 진디혹파리(Aphidoletes aphidimyza Rondani)의 모든 발육단계에 얼마나 영향을 주는가를 평가하였다. 진디혹파리 알에 대한 살충제의 독성평가 결과 emamectin benzoate 등 22종의 살충제가 낮은 독성을 보였다. 알에 독성이 낮은 22종 살충제 중 flonicamid를 포함하는 12종 살충제가 진디혹파리 유충에 대하여 0~27%로 낮은 독성을 보였으나, indoxacarb + teflubenzuron와 chlorantraniliprole의 독성은 각각 35.4%와 64.6%였다. 진디혹파리 유충에 보통독성 이하의 독성을 보인 indoxacarb + teflubenzuron와 chlorantraniliprole을 포함한 14종 살충제는 진디혹파리의 번데기와 성충에도 낮은 독성을 보였다.

Prey species should avoid areas where predation risk is high. However, if this is impossible, prey should reduce activities that may make them conspicuous and attract predators, such as foraging or mating. Thus, predation risk should change behavioral pattern of prey species. Not all species have same anti-predator behavioral patterns because they have evolved in the presence of different types or number of predators in their habitat. In this study we measured microhabitat use and escape initiation distance to identify how sensitive each species is to approaching predators. We measured jumping performance and morphological characteristics to reveal the relationship between jumping and morphology and whether jumping is helpful for escaping from predators' attack. Finally, we compared the survival rate among three species to identify how survival rate is affected by anti-predator behavioral patterns. The survivorship was related to microhabitat use and to the escape initiation distance, rather than on the jumping ability. We predicted that a species with the best survival rate will have superior jumping ability in order to escape from predators at the moment when they were attacked by predators. The jumping ability, however, was probably limited by hydrodynamic and morphological constraints, so jumping appears to contribute little to successfully escaping from predators’ attacks.

Understanding the predator-prey dynamics is essential to comprehend the ecosystem resilience and stability because ecosystems consist of dynamically interacting subsystems with predator-prey relationship. The relationship is likely to be of the predator and prey hunting-escaping strategy. Thus, to better understand the ecosystems, we should comprehend how the hunting and the escaping strategy affect the ecosystems. To do so, we constructed a spatially explicit lattice model to simulate the integrative predator-prey-plant relationships. When an individual simultaneously encounters its predator and/or prey, the individual should take priority between the two strategies. When the hunting (or escaping) strategy takes priority, we call it hunting preferred strategy, HPS, (or escaping preferred strategy, EPS). Each strategy was characterized by the willingness for each strategy. The degree of willingness was represented as H (for hunting) and E (for escaping). Higher value of H (or E) means stronger willingness for hunting (or escaping). We investigated the population density of each species for different values of H and E for HPS and EPS. The main conclusion that emerges from this study was that HPS plays a positive role in the ecosystem stability. In addition, we briefly discussed the development of the present model to be used to understand the predator-prey interaction in specific species.

동물플랑크톤의 서식 형태에 따른 구분(부착 및 부유 성)과 월별 밀도 양상을 파악하기 위해 2011년 5월부터 10월까지 수생식물이 발달된 환경에서 동물플랑크톤 채 집을 수행하였다. 조사 기간 동안, 부착성 동물플랑크톤 은 총 24종, 부유성 동물플랑크톤은 총 30종으로 나타났 으며, 이 중 두 서식 형태 간에 일치하는 종은 20종으로 확인되었다. 부착성 동물플랑크톤은 5~7월 동안 높은밀도를 나타낸 반면, 부유성 동물플랑크톤은 9~10월 동 안 높은 밀도를 보여 상이한 계절적 차이를 보였다. 안정 동위원소 분석 결과, 부착 및 부유입자 유기물, 동물플랑 크톤, 어류의 δ13C와 δ15N 값은 계절별로 상이하였다. 부 착성 동물플랑크톤은 봄, 가을 모두 부착입자 유기물에 대한 높은 의존도를 보였으나, 가을에는 부유입자 유기물 에 대해서는 일부 의존하는 것으로 나타났다. 이는 수생 식물의 우점 등으로 인한 먹이질의 감소와 연관되어 있 을 것으로 사료된다. 그러나 부유성 동물플랑크톤은 봄과 가을 모두 부유입자 유기물에 대해서만 의존하는 것으로 나타났다. L. macrochirus는 크기에 따라 먹이원에 대한 다른 섭식 양상을 보였는데, 부착성 동물플랑크톤이 증가 된 시기(봄)에 1~3 cm 크기의 L. macrochirus는 부착성 동물플랑크톤에 대한 높은 의존도를 보였으나, 가을에 부 착성 동물플랑크톤 감소하자 부유성 동물플랑크톤에 대 한 의존도가 높아지는 것을 확인할 수 있었다. 4~7 cm 크기의 L. macrochirus는 상대적으로 큰 먹이원인 부유 성 동물플랑크톤에 높은 의존도를 보였으며, 가을에 부유 성 동물플랑크톤 밀도가 증가하자 매우 높게 의존하는 것으로 나타났다. 결론적으로 습지와 같이 수생식물이 우 점되는 생태계에서 동물플랑크톤은 다양한 서식 형태(부 착 및 부유성)를 가진 군집이 출현하는 것으로 파악되었 으며, 이들은 각각 다른 먹이원에 대해 의존하는 것으로 나타났다. 또한 상위포식자인 L. macrochirus 또한 부착 및 부유성 동물플랑크톤의 계절별 밀도 양상에 따라 상 이한 의존성을 보였으며, 특히 부착성 동물플랑크톤은 봄 철 치어 성장 시기의 이들의 성장 및 발달에 매우 중요 한 영향을 미치는 것으로 사료된다.

Many animals are capable of detecting sensory information emitted by predators. When predation risk is high, orb-web building spiders should change its foraging strategy of the web construction. We examined web building behaviors of an orb-web spider, Argiope bruennichi providing sensory information of its predator, a yellow hornet, Vespa simillima. We observed web characteristics after providing sensory information of the yellow hornet (experimental group; N=29) and compared to the control group (no information provided; N=34). We hypothesized that the spiders in the experimental group would decrease investments in its web construction and stabilimentum decoration to avoid being located by the predator. The results rejected our hypothesis. Web and stabilimentum constructions decreased in the course of the experiment in both control group and experimental group. But the differences between control group and experimental group were not significant. The individuals did not reduce its stabilimentum length and did not decrease its web area in spite of sensory information of the predator.

The Kanzawa spider mite, Tetranychus kanzawai (Acari: Tetranychidae) constructs webs over leaf surfaces and usually lives under the webs. T. kanzawai produces two types of excreta, black and yellow pellets, and uses its webs as a place for excretion. T. kanzawai also uses its webs as a refuge when the predatory mite, Neoseiulus womersleyi (Acari: Phytoseiidae) is present. To clarify what factors deter N. womersleyi from foraging on T. kanzawai webs, I experimentally examined the effects of T. kanzawai excreta on its own fitness (fecundity) and the foraging behaviour of N. womersleyi. When the excreta of T. kanzawai was put on leaf surfaces, the fecundity of T. kanzawai adult females was reduced by the black but not the yellow faecal pellets. On the other hand, predation by N. womersleyi was reduced by the yellow but not the black pellets. Although this effect of the yellow pellets on N. womersleyi did not last on leaf surfaces, it deterred N. womersleyi from staying on the webs regardless of its freshness. These results suggest that T. kanzawai deposits black pellets on webs to avoid its negative effect on their own fitness, and yellow pellets are deposited on webs to reduce the risk of predation.