The occurrence of exotic weeds and their influx into farmlands due to climate change poses many problems. Therefore, it is necessary to generate a prediction model for the occurrence pattern of these exotic weeds based on scientific evidence and devise prevention measures. The photosynthetic apparatus is known as the most temperaturesensitive component of a plant cell and its initial response to temperature stress is to inhibit the activation of photosystem II. This study investigated the potential of OJIP transients in assessing temperature stress in exotic weeds. The four exotic weeds currently flowing into Korean farmlands include Amaranthus spinosus, Conyza bonariensis, Crassocephalum crepidioides, and Amaranthus viridis. These weeds were treated at 5°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, and 40°C and the OJIP curves and JIP parameters were measured and analyzed. The results showed that heat and chilling stress affected the photosystem II (PSII) electron transport of A. spinosus, whereas C. crepidioides and A. viridis were more affected by high-temperature stress than by low-temperature stress. Lastly, C. bonariensis showed resistance to both high and low-temperature stress. The results of this study suggest that OJIP transients and JIP parameters can be used to analyze damage to the photosynthetic apparatus by temperature stress and that they can serve as sensitive indicators for the occurrence pattern of exotic weeds.

Recently, drones are widely used for various purposes. Among them, it has long been used for military purposes. A drone is an effective asymmetrical power that can strike opponents at low cost. On the other hand, Jammer to subdue drones are also being developed and put into practical use. In this study, a study was conducted to ensure that the weapon system was equipped with an additional Jammer at low cost, and to respond to threats to small drones and drones, by securing an shock resistance t through the transient response analysis of the shooting impact.

To apply CNN to a fluid problem, we need a method to effectively convert the physical quantities of fluid into an image. The performance of CNN was evaluated using the image transformation method using the minimum and maximum values of the pressure distribution data and the image transformation methods using the normal distribution of the pressure distribution data. Through the performance evaluation of the learned CNN, the image transformation methods of Method 4 and Method 5, which applied the normal distribution of representative pressure distribution data, were very effective. In particular, Method 5 includes the initial and final pressure distribution data to include overall pressure distribution data, thereby improving the resolution of the color map to improve classification performance.

The numerical analysis of two-dimensional transient flow around the obstacle with rotated square cross sections was carried out. The obtained velocity distributions for each time step and each rotation angle were imaged to provide data for CNN(convolutional neural network). Both classification and regression neural networks were used for prediction of rotation angle. As results The classification method incorrectly predicted the rotation angle in only 2 of the 470 images. The regression method predicted the rotation angle errors within except 2 out of 470 images. From these facts, it could be concluded that both methods can be sufficiently applicable to the flow analysis.

선박 축계를 구성하는 프로펠러축은 엔진출력, 프로펠러 하중 및 편심추력의 영향으로 인해 거동의 양상이 달라져 선미관 후 부베어링의 국부하중 변화를 일으킴으로써 선미관 베어링 손상의 위험을 증가시킨다. 이를 방지하기 위해 수행된 추진축계 정렬연구는 선급강선규칙을 중심으로 주로 축과 지지베어링간의 상대적 경사각과 유막유지를 최적화 하는데 중점을 두어 진행 되어왔다. 그러나 보다 상세한 평가를 통한 추진축계의 안정성 확보를 위해서는 전타와 같은 급격한 선미유동장 변화에 기인한 과도상태를 포함한 동적상태의 고려가 필요하다. 이러한 관점에서, 본 연구는 50,000 DWT 선박을 대상으로 스트레인 게이지법을 이용하여 밸러스트 흘수 상태에서 정격회전수로 운전 중 대표적 동적 과도상태인 우현 전타상태에서의 프로펠러축 거동이 추진축계에 미치는 영향을 분석하였다. 그 결과 변동된 프로펠러 편심추력은 프로펠러축을 일시적으로 강하게 내려 누르는 힘으로 작용하여 선미관 베어링의 국부하중을 증가시켜 축계 안정성에 부정적 영향을 미침을 증명하였다.

This study examined the effects of freshwater discharge by artificial dikes from the Kanwol and Bunam lakes on the dynamics in the Chunsu Bay, Yellow Sea, Korea, during the summer season based on three-dimensional numerical modeling experiments. Model performances were evaluated in terms of skill scores for tidal elevation, velocity, temperature, and salinity and these scores mostly exceeded 90 %. The variability in residual currents before and after the freshwater discharge was examined. The large amount of lake water discharge through artificial dikes may result in a dramatically changed density field in the Chunsu Bay, leading to an estuarine circulation system. The density-driven current formed as a result of the freshwater inflow through the artificial dikes (Kanwol/Bunam) caused a partial change in the tidal circulation and a change in the scale and location of paired residual eddies. The stratification formed by strengthened static stability following the freshwater discharge led to a dramatic increase in the Richardson number and lasted for a few weeks. The strong stratification suppressed the vertical flux and inhibited surface aerated water mixing with bottom water. This phenomenon would have direct and indirect impacts on the marine environment such as hypoxia/anoxia formation at the bottom.

The flow analysis of two dimensional transient flow over the obstacles with rectangular cross sections was performed. And 190 velocity distributions for each aspect ratio were imaged to provide input data for convolutional neural network learning. The classification and regression methods were used in estimating the aspect ratio from given velocity distributions. As a result the classification method was more exact than the regression method. But both the classification and regression methods gave relatively accurate prediction of the defined aspect ratio judging from the imaged velocity distributions. This confirms that the deep learning technique is applicable to the flow analysis.

The Agrobacterium tumefaciens mediated gene transfer is widely used to generate genetic transformation of plants and transient assay of temporal exogenous gene expression. Syringe infiltration system into tobacco (Nicotiana benthamiana) leaves is a powerful tool for transient expression of target protein to study protein localization, protein-protein binding and protein production. However, the protocol and technical information of transient gene expression, especially double strand RNA (dsRNA), in tobacco using Agrobacterium is not well known. Recently, dsRNA is crucial for insecticidal effect on destructive agronomic pest such as Corn rootworm. In this study, we investigated the factor influencing the dsRNA expression efficiency of syringe agro-infiltration in tobacco. To search the best combination for dsRNA transient expression in tobacco, applied two Agrobacterium cell lines and three plant vector systems. The efficiency of dsRNA expression has estimated by real-time PCR and digital PCR. As a result, pHellsgate12 vector constructs showed the most effective accumulation of dsRNA in the cell. These results indicated that the efficiency of dsRNA expression was depending on the kind of vector rather than Agrobacterium cells. In summary, the optimized combination of transient dsRNA expression system in tobacco might be useful to in vivo dsRNA expression for functional study and risk assessment of dsRNA.

Pb(Zr,Ti)O3 (PZT) is used for the various piezoelectric devices owing to its high piezoelectric properties. However, lead (Pb), which is contained in PZT, causes various environment contaminations. (K,Na)NbO3 (NKN) is the most well-known candidate for a lead-free composition to replace PZT. A single crystal has excellent piezoelectric-properties and its properties can be changed by changing the orientation direction. It is hard to fabricate a NKN single crystal due to the sodium and potassium. Thus, (Na,K)NbO3-Ba(Cu,Nb)O3 (NKN-BCuN) is chosen to fabricate the single crystal with relative ease. NKNBCuN pellets consist of two parts, yellow single crystals and gray poly-crystals that contain copper. The area that has a large amount of copper particles may melt at low temperature but not the other areas. The liquid phase may be responsible for the abnormal grain growth in NKN-BCuN ceramics. The dielectric constant and tan δ are measured to be 684 and 0.036 at 1 kHz in NKN-BCuN, respectively. The coercive field and remnant polarization are 14 kV/cm and 20 μC/cm2.

Transient ischemic attack (TIA) indicates high risk for major stroke and is considered a medical emergency. Diffusion-weighted imaging (DWI) enables detection of acute ischemic lesions. The clinical significance of DWI positive lesions in TIA is obscure and its prevalence, clinical features are not established. Therefore, we performed a clinical, etiological and prognostic analysis through a cross-sectional analysis of 235 TIA patients, grouped according to presence of DWI lesion. Clinical features, underlying risk factors for stroke, outcome and rate of recurrence were analyzed. 3 months follow-up of modified Rankin Scales (mRS) were done with telephone survey. DWI positive lesions were present in 14.0% of patients. Etiological factors significantly associated with DWI lesions in TIA patients were male sex (p = 0.038), stroke history (p = 0.012) and atrial fibrillation (p < 0.001). Presence of at least one medium or high risk of cardioembolism from TOAST classification were not associated with lesions when excluding association to atrial fibrillation (p = 0.108). Clinical features showed no significant difference. Whether the patients had lesion-positive DWI was not related to an increase in mRS score during the hospital stay or at the 3-month follow-up after discharge. Future studies should include multi-center samples with large numbers, considering each unique medical environment. Routine acquisition of follow-up DWI for proper evaluation of the tissue-based definition of TIA should also be considered.

선박 축계는 프로펠러 하중의 영향으로 선미관 후부베어링의 국부하중 증가가 현저히 나타나 축계 선미관 베어링 손상의 위험이 증대된다. 이를 방지하기 위해 수행된 추진축계 정렬연구는 주로 준정적 상태(quasi-static condition)에서 축과 지지베어링간의 상대적 경 사각을 감소시키는데 중점을 두어 진행되어 왔다. 그러나 보다 상세한 평가를 위해서는 동적상태를 추가로 고려하는 것이 필요하다. 4,70 0 DWT 선박을 대상으로 NCR로 운전중 급속으로 우현 전타할 때 추진축계가 받는 영향에 대해 연구하였다. 연구결과 선미 유동장 변화 에 의해 프로펠러 편심추력이 과도 상태가 되어 프로펠러에 불평형 진동이 유발되는 것을 확인하였다. 우현 전타시의 프로펠러 편심추력은 NCR 조건대비 축을 선미관 베어링으로부터 들어 올리는 힘으로 작용하여 선미관 베어링 하중완화에 기여하고 있음을 확인하였다.

이 논문에서는 스펙트럴 요소법과 외연적 시간적분법을 이용해 SH파의 전파 거동을 계산하는 수치해석 기법을 제시한다. 2차원 영역에서의 탄성파 해석을 위해 해석영역을 유한 영역으로 한정하고 파동이 반사되지 않도록 수치적 파동흡수 경계조건인 perfectly matched layer(PML)를 도입하였다. PML이 포함된 시간영역 파동방정식의 유한요소해법을 위해 스펙트럴 요소법을 적용하였고 Legendre- Gauss-Lobatto 수치적분법을 사용하여 질량행렬을 대각화하였다. 2차 미분방정식 시스템의 파동방정식을 1차 미분방정식 시스템으로 변환하였고 병렬화를 통한 탄성파 해석 성능의 최적화를 위해 외연적 시간적분법인 4차 Runge-Kutta 방법을 이용해 해석영역에서의 변위응답을 계산하였다. 2차원 해석영역에서 SH파의 전파 거동을 계산하는 수치예제를 통해 제시한 외연적 스펙트럴 요소법의 정확성을 검증하였고 PML로 인한 반사파의 감쇠효과를 확인하였다. 외연적 시간적분법을 통한 탄성파 해석 기법은 3차원 영역과 같은 대규모 문제에서의 탄성파 수치해석을 효율적으로 수행할 수 있을 것으로 기대된다.

The virus-like particle (VLP) is similar to a pathogenic virus and has a high immunogenicity. However, in the selection of various structural proteins to form VLP, all expression systems commonly consume most of the time and suffer from various difficulties. Therefore, there is a need for a system that can rapidly determine the structural proteins required for effective VLP production. This study aims to construct a transient expression platform using insect cells to solve this problem. Plasmid-based expression vectors and baculovirus-inducible expression vectors were constructed. The vectors were evaluated for overexpression using EGFP. We also confirmed the formation of virus like particles through overexpression of virus structural proteins.

Transient receptor potential melastatin 8 (TRPM8) plays a crucial role in innocuous cool sensation, acute cold pain and cold-induced hyperalgesia during pathologic conditions. To help understand TRPM8-mediated cold perception in the dental pulp and periodontal tissues, we examined the distribution of TRPM8-immunopositive (+) axons in molar and incisor pulp and periodontal tissues using transgenic mice expressing a genetically encoded axonal tracer in TRPM8+ neurons. In the radicular pulp of the molar teeth, a small number of TRPM8+ axons were observed. TRPM8+ axons branched frequently and extensively in the core of coronal pulp, forming a network in the peripheral pulp. Some TRPM8+ axons ascended between odontoblasts and were observed in the dentinal tubule. TRPM8+ axons were linear-shaped in the radicular pulp, whereas many TRPM8+ axons showed portions shaped like beads connected with thin axonal stands at the peripheral pulp. TRPM8 was densely expressed in the bead portions. In the incisor pulp, TRPM8+ axons were occasionally observed in the core of the coronal pulp and rarely observed at the peripheral pulp. TRPM8+ axons were occasionally observed and showed a linear shape rather than a bead-like appearance in the periodontal ligament and lamina propria of the gingival tissue. These findings, showing differential distribution of TRPM8+ axons between radicular and coronal portions of the molar pulp, between incisor and molar pulp, and between dental pulp and periodontal tissues, may reflect differential cold sensitivity in these regions.