논문 상세보기

Development of molecular marker linked to white rust resistance in chrysanthemum using bulked segregant analysis (BSA)

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/297695
서비스가 종료되어 열람이 제한될 수 있습니다.
한국육종학회 (The Korean Breeding Society)
초록

For developing molecular markers linked to white rust resistance in chrysanthemum, RAPD and AFLP were carried out in ‘Puma White’ x ‘Dancer’ mapping population through Bulked Segregant Analysis (BSA) methods. 10 resistant and 10 susceptible individuals were selected and bulked. And then, these bulks were screened using 280 RAPD primers (10 mer) with two parents. As a result of BSA-RAPD, 25 Dancer/R-bulk specific bands in 21 primers and 22 Puma White/S-bulk specific bands in 18 primers were selected. These resistant or susceptible specific bands were screened in 10 resistant and 10 susceptible individuals. Except OPI-13520, all bands were confirmed as false positive. OPI-13520 band presumed as closely linked marker to white rust disease resistance was tested in whole population. Among 187 progenies, just six off-springs did not correspond with phenotypic data. Based on expected phenotypic segregation ratios in the pseudo F1 progenies, it was assumed that a duplex type of white rust resistance in ‘Dancer’ (RRrrrr) were in combination with a duplex type of OPI-13520 marker. As a result of x2-test of independence between resistance gene and OPI-13520 marker, x2 score is 76.08 and probability is 2.13x10-16. And resistance gene and OPI-13520 marker were assumed to be linked in coupling phase. The value of recombination fraction obtained by successive trials and second derivative of log likelihood was 0.03832±0.0271.

저자
  • Sang-Kun Park(Floricultural Research Division, National Institute of Horticultural and Herbal Science) Corresponding Author
  • Jin-Hee Lim(Plant Engineering Research Institute, Sejong University)
  • Jae-A Jung(Floricultural Research Division, National Institute of Horticultural and Herbal Science)
  • Young Soon Kwon(Floricultural Research Division, National Institute of Horticultural and Herbal Science)
  • Hak-ki Shin(Floricultural Research Division, National Institute of Horticultural and Herbal Science)