KOREASCHOLAR

Purpose: The objective of this study was conducted to investigate the effects of rutin, buckwheat components on cell growth and anti-inflammation in adipocyte 3T3-L1 and human colon cancer cell SW-480. Methods: We cultured 3T3-L1 adipocyte and SW-480 colon cancer cell to confluence, at which time starvation was induced with SFM for 1 day. Cells were then cultured in medium containing 0, 25, 50, or 100 μmol/mL of rutin 3T3-L1 or 0, 10, 20, or 40 μmol/mL SW-480. Cell viability was measured using a cell viability kit. In addition, we examined the expression of mRNA related to inflammation. RT-PCR was used to quantity tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), IL-6, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) mRNA levels. Results: Rutin significantly inhibited 3T3-L1 and SW-480 cell proliferation in a dose and time dependent manner. Rutin also significantly reduced the mRNA expression of IL-1β, IL-6 and TNF-α at the highest dose. In addition, rutin treatment caused a significant reduction in COX-2 and iNOS mRNA levels compared to the control group. Conclusion: Overall, our results suggest that rutin has the potential to reduce inflammation, and that these effects are greater during tissue-damaging inflammatory conditions.

Abstract
 I. 서 론
 II. 재료 및 방법
  1. 시료의 준비
  2. 세포 배양과 지방세포로의 분화
  3. 세포증식
  4. 염증유전자의 mRNA 발현
  5. 통계 처리
 III. 결과 및 고찰
  1. 세포증식에 미치는 영향
  2. 염증매개 사이토카인 mRNA 유전자 발현에 미치는 영향
 IV. 요약 및 결론
 References

• 이승림(상지영서대학교 식품조리과) | Suenglim Lee (Department of Food and Cooking, Sangji Youngseo College)
• 서은영(장안대학교 식품영양과) | Eunyoung Seo (Department of Food and Nutrition, Jangan University) Corresponding author