KOREASCHOLAR

Ulcerative colitis (UC) is a chronic inflammatory intestinal disease characterized by an imbalance in immune function and the overexpression of inflammatory cytokines and mediators. Vitamin B2, also known as riboflavin (Libof), is an essential water-soluble vitamin with numerous beneficial properties, including antioxidant, anti-aging, anti-inflammatory, antinociceptive, and anti-cancer effects. In this study, we aimed to investigate the protective effects of Libof on dextran sulfate sodium (DSS)-induced experimental colitis. The C57BL/6 mice were used as the in vivo model of chronic colitis to investigate the anti-inflammatory effects of Libof. RAW 264.7 cells were used for the in vitro investigation of the molecular mechanisms underlying these effects. In vivo, Libof alleviated the DSS-induced disease activity index (DAI), colon length shortening, and colonic pathological damage. In vitro, Libof inhibited lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 production in RAW 264.7 cells. Moreover, Libof inhibited LPS-induced nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells. In conclusion, these findings indicate that Libof shows potential as an agent for the treatment of UC.

I. 서 론
II. 연구 내용 및 방법
    1. 실험동물
    2. Dextran sulfate sodium(DSS)에 의한 만성 대장염의 유발 및 시료의 경구 투여
    3. DSS로 유도된 대장염 모델의 질병 활성화 지수 평가
    4. 장기 적출 및 대장 길이 측정
    5. H&E를 통한 조직학적 분석
    6. 세포 배양 및 세포 처리
    7. 세포 생존율
    8. 단백질 발현 측정
    9. NO 발현 저해능 측정
    10. 사이토카인 발현 저해능 측정
    11. 통계처리
III. 결과 및 고찰
    1. DSS로 유도된 대장염에 대한 리보플라빈의 질병 활성화지수에 대한 효과
    2. DSS로 유도된 대장염에 대한 리보플라빈의 대장 길이 증가 효과
    3. DSS로 유도된 대장염에 대한 리보플라빈의 조직학적 변화 분석
    4. RAW 264.5 cell에서 리보플라빈의 세포 독성 평가
    5. RAW 264.5 cell에서 리보플라빈의 염증성 사이토카인 생성 억제 효능
    6. RAW 264.5 cell에서 리보플라빈의 NO 생성, iNOS 발현억제 효능
IV. 요약 및 결론
감사의 글
Conflict of Interest
References

• 이상희(한국식품연구원) | Sang Hee Lee (Korea Food Research Institute)
• 홍선미(환동해산업연구원) | Sun Mi Hong (Marine Industry Research Institute for East Sea Rim)
• 성미정(한국식품연구원) | Mi Jeong Sung (Korea Food Research Institute) Corresponding author