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Study on the Reproductive Function in Transgenic Pig Harboring Human Erythropoietin (hEPO) Gene

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  • URLhttps://db.koreascholar.com/Article/Detail/109712
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한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

Our previous study showed that transgenic (TG) pigs harboring human EPO (hEPO) gene have been shown to have reproductive disorders, including low pregnancy rates, irregular estrus cycle and low little size. To investigate these reasons, we assessed estrus behavior (standing response) and plasma 17B-estradiol (E2) level, which partly reflect reproductive function, during the estrus cycles after synchronization and superovulation by hormone treatments. Then, we analysed blood composition and expression of hEPO gene in TG pigs. Pigs were injected with PG600. After 10 days, pigs were fed with Regumate porcine for 6 days. Blood samples were collected from jugular vein. Analysis of blood composition and E2 level were measured by Hemavet 950 and E2 ELISA kit, respectively. And, the expression of hEPO gene in reproductive organs was quantitated by real-time RT-PCR. The percentage of estrus behavior in TG was significantly decreased. Hematocrit (HCT), hemoglobin (Hb) concentration and red blood cell (RBC) number were significantly higher in TG than wild type (WT). On the other hand, high expression of hEPO gene in TG was observed in the mammary gland as well as in the uterus. Moreover, plasma E2 level was significantly higher in TG than WT. These results suggest that nonspecific expression of hEPO gene in the other organs of TG may affect blood composition and plasma E2 level, thereby causing reproductive disorders.

목차
ABSTRACT   INTRODUCTION   MATERIAL AND METHODS   RESULTS   DISCUSSION   REFERENCES
저자
  • Hyun-Gi Lee(Animal Biotechnology Division, National Institute of Animal Science, RDA, Animal Genetic Resurces Station, National Institute of Animal Science, RDA)
  • Hwi-Cheul Lee(Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • Hak-Jae Chung(Hanwoo Experimental Station, National Institute of Animal Science, RDA.)
  • In-Sul Hwang(Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • Myoung-Seob Choi(Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • Sung-June Byun(Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • Seunghoon Lee(Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • Min-Ji Kim(Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • Jae-Seok Woo(Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • Won-Kyong Chang(Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • Poongyeon Lee(Animal Genetic Resources Station, National Institute of Animal Science, RDA)
  • Hoon-Taek Lee(Department of Animal Science, Konkuk University) Corresponding author
  • Jin-Ki Park(Animal Biotechnology Division, National Institute of Animal Science, RDA) Corresponding author