논문 상세보기
pp.59-67
초기배아의 발생과정동안 배아와 모체에서 발현되는 여러 cytokine은 착상을 위한 신호물질로 중요한 역할을 한다. 그 중 interleukin-1 (IL-1)는 배아와 모체간의 상호 신호전달체로서 성공적인 착상을 위한 필수적인 요소로 작용한다고 알려져 있다. 따라서 본 연구에서는 초기배아의 발생과정에 있어서 IL-1 유전자의 역할을 규명하기 위해 생쥐 초기배아에서의 IL-l 유전자의 발현양상을 역전사중합효소연쇄반응을 통해 조사하였고, IL-l 유전자의
To investigate the role of interleukin-l (IL-1) in the embryonic development, in vivo and in vitro expression patterns of IL-1 gene in the preimplantation mouse embryos were examined by RT-PCR, and the effects of explanted mouse ovi-duct and uterine epithelial cells on the expression of IL-1 gene in the pleimplantation mouse embryos were examined by co-culture. IL-1 mRNA was detected in the embryos from 4-cell stage to blastocyst stage in vivo and from morula stage to hatching blastocyst stage in vitro. This transcript was not detected from the GV stage to late 2-cell stage in vivo, and not at the 4-cell and 8-cell stages in vitro. For the co-culture of late 2-cell embryos with the explanted mouse oviduct and uterine epithelial cells, oviducts and uterine epithelial cells were isolated at 48 hour alter the hCG injection. The explanted oviduct and uterine epithelial cells in co-culture groups facilitated the IL-1 gene expression of the mouse embryos in comparison with the control. Taken together these results suggest that the presence of IL-1 plays an important role in preimplantation embryonic development. In addition, the up-regulation of IL-1 gene expression by the explanted oviduct and uterine epithelial cells demonstrates that embryonic expression of IL-l gene may be regulated by the interaction with oviductal and uterine factor (s).
