The techniques of IVM, IVF and IVC of canine oocytes may provide useful information for gamete salvage programs and the conservation of endangered canidae. This investigation has been made to determine the efficiency of in vitro maturation (IVM) as a basic experiment to study the development of canine oocytes after in vitro fertilization (IVF). The rate of oocytes developing to the MII stage was higher in the hormone treated group (10 IU/ml hCG+eCG, 14.7%, p<0.05) than in the control group (0 IU/ml hCG+eCG, 10.0%). The monospermy and pronuclear rates of canine oocytes were investigated after caffeine treatment on IVF. Canine oocytes were fertilized in the Fert‐TALP medium supplemented with 0, 10, 20 or 30 mM caffeine (Fert I, Fert II, Fert III or Fert IV, respectively). The highest pronuclear formation rate was obtained in the Fert I for 24 h IVF (6.7%, 6/89). Therefore, it is believed that unlike in other mammals, caffeine in canine IVF does not increase the efficiency of fertilization rate, and is not an important factor.

ABSTRACT   INTRODUCTION   MATERIALS AND METHODS   RESULTS   DISCUSSION   REFERENCES

• Bong Soo Kim(School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea)
• Dong Hoon Yoo(School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea)
• Mi Jung Shin(School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea)
• Hae Jung Kim(School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea)
• Dong Seok Lee(School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea)
• Sanggyu Lee(School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea)
• Ji Hong Ha(School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea)
• Zae Young Ryoo(School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea) Corresponding author
• Byung-Hwa Hyun(Disease Model Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, South Korea)
• Young Sik Park(Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701, South Korea)