The purpose of this study is to establish a basic culture system enabling in vitro culture of chicken blastodermal cells and to test the feasibility of retrovirus-mediated gene transfer to the cultured cells. The blastodermal cells were isolated from freshly laid eggs of stage X and cultured with or without STO feeder layer cells. Stem cell-like morphology was maintained after multiple passages and RT-PCR analysis proved expression of several stem cell specific genes. Immunocytochemical analysis using antibodies of anti-EMA-1 and anti-SSEA-1 also showed the feature of stem cells. Infection of the cultured blastodermal cells with LNCGW retrovirus vector resulted in successful transfer of foreign genes. The results of this study may be useful in establishing stem cell-mediated transgenic chicken production.

ABSTRACT   서론   실험 방법    닭의 배반엽 세포 분리 및 일차배양    분리한 배반엽 세포의 계대배양    닭 배반엽 세포의 분자 생물학적 검정   결과    배반엽 세포의 In Vitro 배양    배아 줄기세포 여부에 대한 분자 생물학적 및 세포면역학적 검정    Retrovinus Vector System을 이용한 외래 유전자 전이   고찰   인용문헌

• 박성준(대구가톨릭대학교 외과대학 생리학교실) | Sung Joon Park
• 구본철(대구가톨릭대학교 외과대학 생리학교실) | Bon Chul Koo
• 권모선(대구가톨릭대학교 외과대학 생리학교실) | Mo Sun Kwon
• 김태완(대구가톨릭대학교 외과대학 생리학교실) | Teoan Kim Corresponding author