The purpose of this study was to improve of frozen-thawed sperm using magnetized water in Korean native cattle. Before cryopreservation, without egg-yolk Triladyl® solution was flowed though magnet [0, 2000, 4000 and 6000 Gauss(G)] for 5 min. The freezing of dilluted semen added with Triladyl containing 20% egg-yolk. Analysis of frozen-thawed sperm was estimated viability with SYBR14/PI double stain, membrane intact with hypoosmotic swelling test (HOST), acrosome reaction with FITC-PNA, mitochondria membrane function with Rhodamin 123 by flow- cytometry. Sperm viability was significantly higher in 4000G group than other groups (p<0.05). However, the Hypoosmotic Swelling Test(HOST) was significantly higher in fresh, 4000 and 6000G than 0 and 2000G (p<0.05). In addition, mitochondria membrane damage and acrosome damage were significantly lower in 6000G group than other groups (p<0.05). in conclusion we suggest that magnetized water could be improve ability of sperm on cryopreservation in Korean native cattle.

ABSTRACT   서론   재료 및 방법    정액 채취    정액 동결    동결정액 융해 및 성상검사    생존율 검사(SYBR-14/PI Staining)    정자원형질막 기능 검사(Hypoosmotic Swelling Test)    미토콘드리아 검사(Rhodamin123/PI Staining)    첨체검사(FITC-PNA/PI Staining)    통계처리(Statistical Analysis)   결과    자기활성된 동결액 처리 후 생존율 평가    정자의 원형질막 검사(Hypoosmotic Swelling Test)    정자의 미토콘드리아 검사    자기활성된 동결액 처리 후 정자의 첨체 손상 검사   고찰   인용문헌

• 서기범(강원대학교 동물생명과학대학) | Gi-Beom Seo
• 이용승(강원대학교 동물생명과학대학) | Yong-Seung Lee
• 이경진(강원대학교 동물생명과학대학) | Kyung-Jin Lee
• 유한준(강원대학교 동물생명과학대학) | Han-Jun Yu
• 정희태(강원대학교 수의학과) | Hee-Tae Cheong
• 양부근(강원대학교 동물생명과학대학) | Boo-Keun Yang
• 이승환(국립축산과학원 한우시험장) | Seung-Hwan Lee
• 이진우(ROHASTECH) | Jin-Woo Lee
• 박춘근(강원대학교 동물생명과학대학) | Choon-Keun Park Corresponding author