This study was carried out the estimation on consumption patterns and consciousness of domestic forage for improvement of the quality of domestic forage. Although the cultivated area in South Korea of forage has increased significantly compared to the past, the self-sufficiency rate of domestic forage has increased to around 80% since 2010. Also, livestock farmers prefer to use import forage than domestic due to convenience of use. In Korean beef farms, the ratio of import to domestic forage was higher in domestic forage (import forage 3 : domestic forage 7). In the method of securing domestic forage, purchase of forage (55.6%) was higher than self-cultivation of forage (44.4%). The ratio of use by bailing type was shown in the order of rice staw rice straw (50.5%), domestic hay (15%), imported hay (12.5%), and total mixed ratio (10.7%). The preference of forage was in the order of amount of foreign matter, moisture content, price, feed value in Korean native cattle farm. The result of satisfaction with domestic and import forage showed that the satisfaction of domestic forage price was higher than import forage, while the moisture content and foreign matter of forage were lower than import forage. In addition, in the results of the satisfaction and importance of domestic roughage compared to imported roughage, satisfaction with imported roughage was generally high in all items except for price. As a result, in order to improve the satisfaction of domestic forage in Korean native cattle farm, it is necessary to minimize foreign matter in forage and increase hay production for moisture content uniform in forage.

The vomeronasal organ (VNO) is critical for reproduction and social behavior in ruminants, including cattle. The present study examined the structure of the VNO and its epithelial cells in neonatal and adult Korean native cattle (Hanwoo), Bos taurus coreanae , using immunohistochemistry and lectin histochemistry. Histologically, the VNO comprised two types of epithelia: medial vomeronasal sensory (VSE) and lateral vomeronasal non-sensory epithelia (VNSE). Numerous blood vessels and nerve bundles were observed within the vomeronasal cartilage encased lamina propria. Immunohistochemistry revealed high expression level of protein gene product9.5 and moderate expression level of olfactory marker protein in the neuroreceptor cells of the VSE and occasionally in some ciliated cells of the VNSE in both neonates and adults. The properties of the glycoconjugates in the VNO were investigated using 21 lectins, most of which were expressed at varied intensities in the VSE and VNSE, as well as in the lamina propria. Several lectins exhibited variations in their intensities and localization between neonatal and adult VNOs. This study is the first descriptive lectin histochemical examination of the VNO of Korean native cattle with a focus on lectin histochemistry, confirming that the VNO of Korean native cattle is differentiated during postnatal development.

Sperm cryopreservation is an important method of assisted reproductive techniques and storing genetic resources. It plays a vital role in genetic improvement, livestock industrial preservation of endangered species, and clinical practice. Consequently, the cryopreservation technique is well organized through various studies, especially on Korean native cattle (Hanwoo). However, the cryopreservation technique of Korean native brindled cattle, which is one of the native cattle species in Korea, is not well organized. Therefore, it is necessary to develop a Supplementary Table technique for the cryopreservation of Korean native brindled cattle. For this purpose, it is important to first evaluate the quality of the currently produced cryopreserved sperm of Korean native brindled cattle. In this study, we randomly selected 72 individual Korean native brindled cattle semen samples collected from 8 different region research centers and used them to evaluate sperm functions. We focused on the quality evaluation of cryopreserved Korean native brindled cattle semen following the measurement of motion kinematics, capacitation status, intracellular ATP level, sperm motility, and cell viability. Then, the values of each of the eight groups were derived from various sperm parameters of nine individual samples, including sperm motility, kinematics, cellular motility, and intracellular ATP levels, which were used to compare and evaluate sperm function. Overall, differences in various sperm parameters were observed between most of the research centers. Particularly, the deviations of motility and motion kinematics were high according to the sample. Therefore, we suggest that it is necessary to develop a standard method for the cryopreservation of Korean native brindled cattle semen. We also suggest the need for sperm quality evaluation of the cryopreserved semen of Korean native brindled cattle before using artificial insemination to attain a high fertility rate.

번식효율은 농가수입에 직접적으로 영향을 미치는 요인으로 암소뿐 만 아니라 씨수소의 번식효율은 중요한 경제형질로 여겨지고 있다. 본 연구에서 는 한우 씨수소에 대한 번식효율을 분석하기 위하여 2010~2017년에 전북지역 분만기록 62,284개와 이에 사용된 132두의 KPN에 따른 인공수정 수태율을 분석하였다. 종부차수(NAIPC)가 증가함에 따라 분만간격(CI), 공태기간(CCI)이 유의적으로 증가하였으며(P<0.05), 임신율은 낮아졌다. 첫 종부 임신율은 62.365%로 나타났으나, 3차 이후에는 48.147 이하로 급격히 낮아졌으며, 6차 이후에는 15.664% 이하로 낮아졌다. KPN과 인공수정사 모두 수태율에 크게 영향을 미치는 것으로 나타났다. KPN에 따른 수태율은 39.154~70.621%로 분석되었으며, 인공수정사에 따른 수태율 은 22.237~85.517%로 나타났다. 인공수정 실패 시 같은 KPN의 재사용율은 20% 내외로 특별한 경향은 보이지 않았다. 첫 종부 기록만을 이용하여 상대적 추정 수태율(ESCR)을 분석한 결과 KPN1041이 가장 높은 11.107%로 나타났으며, KPN1112는 가장 낮은 -20.591%로 나타나 31.698%의 차이를 보였다. 본 연구결과 인공수정사 뿐 만 아니라 전북지역에서 사용되고 있는 KPN에 따라 번식효율이 크게 영향 받을 수 있는 것으로 나타났다.

The fatty acid binding protein 4 (FABP4) gene plays an important role in lipid metabolism and homeostasis in adipocytes. The objective of this study was to analyze the association between a single nucleotide polymorphism (SNP), g.7516G>C, in the FABP4 gene and economic traits of Korean native cattle, Hanwoo. Primers were designed to target a region of the FABP4 gene between nucleotides 7417 and 7868 (AAFC01136716). The SNP, which was detected by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method using restriction enzyme MspA1I, was genotyped in 319 animals of Hanwoo steer population. Statistical analysis showed that the SNP genotype of the FABP4 gene significantly affected carcass weight (CW, p<0.01), longissimus muscle area (LMA, p<0.001), and marbling score (MS, p<0.001). GG allele of the SNP on 246 animals in a Wagyu × Limousin F2 reference population showed a higher MS (p<0.05) and subcutaneous fat depth (p<0.05) in previous report. But CC allele of the SNP showed greater values for MS, LMA, and CW in Hanwoo steers. These results suggest that the g.7516G>C SNP located in the FABP4 gene may affected differently depending on the cattle breed and can be used as a genetic selection marker in Korean native cattle.

한국에 있어서 한우는 경제 동물일 뿐 만 아니라 중요한 유전자원 중 하나이다. 그러나 현재 한우의 번식 능력 개량을 위한 연구는 매우 미흡한 실정이다. 한우의 번식 능력에 대한 기초 자료를 확보하기 위해 전북지역(정읍시)에서 인공수정 된 암소 총 33,281두에 대한 105,017개의 번식기록(2010-2017)을 이용하여 임신기간(GP), 분만 후 첫 종부기간(CFSI), 분만 후 임신간격(CCI), 수태당 종부회수(NAIPC), 첫 종부 임신율 (FSCR)을 계산하여 분석에 활용하였다. 전체적으로 GP 는 287.602±4.797일, CFSI와 CCI는 각각 77.513±34.417 및 88.433±42.194일 이였다. 또한 FSCR는 62.365%였으며, NAIPC 는 1.411회로 나타났다. 2017년의 FSCR과 NAIPC는 각각 67.497% 및 1.344회로 2013년 62.366% 및 1.402에 비해 유의적으로 증가하였다(p<0.05). 가을과 겨울에는 번식기간이 더 길었으며, NAIPC는 봄과 여름에 비해 유의적으로 증가하는 것으로 나타났다(p<0.05). 3~7산차 암소의 경우 NAIPC가 1.323±0.669~1.339±0.689회로 다른 그룹에의 1.401±0.772~1.446±0.854회 보다 우수한 것으로 나타났다. 반면, 1산차 암소의 경우 FSCR와 NAIPC가 각각 60.217% 및 1.506±0.937회로 가장 저조한 것으로 나타났다. 본 연구 결과 한우의 번식효율은 산차에 따라, NAIPC는 계절에 따라 영향 받는 것으로 나타났으며, 전체적인 번식효율은 과거에 비해 지속적으로 향상되고 있는 것으로 나타났다.

This study was conducted to analyze the specific genes associated with sex-determination in semen of Korean Native cow. Male fertility is dependent upon the successful perpetuation of spermatogenesis that is a highly organized process of germ cell differentiation occurring within the seminiferous tubules in the testis. The highly organized spermatogenesis requires accurate, spatial and temporal regulation of gene expression governed by transcriptional, post-transcriptional and epigenetic processes. Recently, the farmers have been interesting in the male or female of calves in the their farm. In first, we analyzed the semen supplied from Hanwoo Improvement Center, NongHyup. The sperm motility in Hanwoo was decreased approximately 10 % in the 30 min after sex-determinant reagent. However, Holstein' sperm motility was decreased to 60-70% after 15 min and the motility was considerably decreased to 20-30 % after 30 min. Next, we analyzed the sperm specific expression genes both male- and female reagents treated-group. The real-time PCR results suggest that the selected genes (GIMP4, TMEFF1, RAC2, ABI2, RAC1, and CLUS) were highly expressed in the group treated with the male reagent compared to female reagent treated group and untreated-group. In the present study, although X or Y gene is play a key role in the sex-determination of mammalian, we suggest that the selected genes may be involved in the sex-determination.

This study is to compare the effect of estrus synchronization and embryo transfer between Korean and Mongolian cattle. Embryos were collected from 9 donors housed in Asan city in South Chungcheong Province, South Korea. Embryos were collected 9 donors from Khushaat sum, Selenge province and Bayanchandmani sum, Tov province in Mongolia. Follicle Stimulating Hormone (FSH), Controlled Internal Drug Release (CIDR) and Prostaglandin (PG) were used for superovulation. Subsequently, Artificial Insemination (AI) was done for donor cow and embryo was collected after 7 and 8 days. Collected embryos were compared between Mongolian and Korean cattle. Finally, good quality and fresh embryos were transferred to 50 and 22 recipients of cows in Korea and Mongolia respectively. The findings show that Korean native cattle each donor cow produced on an average 16.9 embryos and, 10.9 embryos were found transferable. But in case of Mongolia the average production of embryos per donor cow was 8.6 embryos and, 6.2 embryos were found transferable. Embryo collection after 7 and 8 days was not difference in embryo production in Korea. But, in Mongolia embryo production after 8 days was found more efficient than the 7 days. Korean native recipient's cows (74.6%) and Mongolian recipient's cows (71.0%) respectively were found transferable ovarian stage. The result suggested that efficiency of embryo production from the superovulation method treated of Korean cow were higher than the Mongolian cow. The pregnancy rate of Korea native cattle was 72%, which was about 10% higher than that of Mongolia cattle.

This study was conducted to investigate the optimal artificial insemination (AI) time with diagnostic kit at ovulation time. We already applied the patent about the protein in the cow heat mucose in external reproductive tract. And we would examine the accuracy for detection of cow heat by the kit produced with the protein. Evaluation of optimal heat detection was tried two time at 12 hrs and 24 hrs after the heat. And then, AI service also performed two times with no relation to the results of heat diagnosis by heat detection kit and pregnancy rates were checked with rectal palpation on 60th day after AI. Heat diagnostic results by kit in natural heat after 12 hrs in Hanwoo cows were showed 31.3~75.0% on positive in first heat detection and 33.3~100.0% on positve in second heat detection. In the 1st positive results were significant different (p<0.05), but 2nd positive were not. The results of heat detection showed different result on regional influence and individual cow effects. The pregnancy rates of first trial of heat detection were showed 34.4~78.7% on positive and 21.3~68.8% on negative after the diagnosis by heat detection kit. And the pregnancy rates of next trial of heat detection were showed 33.3~85.7% on positive and 14.3~66.6% on negative after the heat diagnosis. Both positive results of first trial and next trial also were showed significant different (p<0.05), but negative results were not. In positive result, first trial of total pregnancy rates was higher than the next trial of pregnancy, but there showed opposite results on negative results. In conclusion, the optimal heat detection kit is suitable to ordinary Hanwoo cows and it suggested that we have to improve the kit’s accuracy by detecting the materials like proteins related optimal AI time.

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis. This study was conducted to clarify the seroprevalence of BLV in the Republic of Korea. Blood samples were obtained from Korean native cattle farms in all provinces of South Korea except Jeju. A commercial indirect enzyme-linked immunosorbent assay with 4,498 samples to detect antibodies against BLV was conducted. The results revealed that the prevalence of BLV was dependent upon age, with increasing prevalence among cattle occurring until they were 5 years old. The highest seroprevalence in cattle was observed in Chungnam (29.6%) and the lowest was observed in Jeonnam (2.6%). The mean overall prevalence for BLV antibodies in the survey was 10.2%, indicating that BLV is widespread nationwide.

Cryopreservation of germ cells from genetically proven animals could be a source of restoration tools from the risk of extinction or disappearance of wanted characteristics. Using frozen semen, the genetic gains of Korean native cattle have been increased greatly for 70 years. The preservation of genetic resources as a form of frozen semen straw has limited availability due to the numbers. To circumvent this weakness of frozen semen, we tested two re-freezing methods with different initial thawing temperatures using frozen Korean proven semen and rare breed semen from albino, black and chikso breeders. It has been known that human sperm could resist to cryo-damages by repeated freeze-thaw cycles, but not for Korean proven bulls number (KPN) or for rare breeds. Total 7 frozen semem from brindled(2), black(1), Korean Albino(2) and KPN(1) bulls were used for our research. After thawing straws under 5°C/2min or 37°C/40sec with low temperature water bath and thermo jug, spermatozoa were re-diluted with triladyl diluents after first thawing and re-frozen. Sperm motilities were compared between animals and treated groups after re-thawing. Mean values of motility and viability of refrozen/thawed sperm for expansion of the number of straws were significantly higher in 5°C than in 37°C (P< < 0.05). However, the activity of viable sperm thawed at 5°C was significantly decreased after first and second thawing. It is estimated that re-freezing of frozen semen from rare Korean native cattle is possible with resistant properties of survived spermatozoa.

The Korean native cattle, Hanwoo, is the most popular breed of beef cattle in Korea. However, the reproductive performance data are limited although reproduction is one of the most economically and biologically important in beef production. Therefore, this study was undertaken to investigate reproductive performance parameters including calving interval, parity for life time production. Data collected from 206,827 calvings were analyzed. There were no significant differences in calving interval and gestation days as parity increased from 2nd and 13rd parity cow, from spring to winter. However, we found a dramatic increase in calving interval after year 2000. About 1 month were increased per year ( y = 30.578x + 344.45 R² = 0.9157). Interestingly, we observed that parities for life time can be affected by birth weight. Calves with 23 kg at birth showed highest parities, 3.4±2.0 times. In summary, this study provides valuable data on reproductive performance of Hanwoo and the data presented here can be used as a standard target for optimising and enhancing reproductive performance.

Wholesale beef price is the critical factor for determining Korean native cattle, Hanwoo, farm’s income in short-term. Wholesale beef price has seasonality due to high demand in Korean traditional holidays such as Korean thanksgiving day and lunar new year’s day. Therefore, it is important to make reproduction and marketing plans for Korean Hanwoo farmers, in order to increase their farm income. However, there is no study available on changes in the expected farm income depending on reproduction and marketing schedules. This study analyzed the expected farm income per head depending on the monthly-based marketing schedules. The analysis was conducted based on the seasonality of wholesale beef price, reproduction efficiency, operating costs, relationship between carcass grade and slaughter age. The result shows that slaughter Hanwoo at the age of 29 months-old in August and January generating the highest expected farm income per head.

The aim of the present study was to evaluate and estimate timing of artificial insemination (AI) in Hanwoo heifer (Korean native cattle) that is the most popular breed of beef cattle in Korea. To determine changes in body weight of heifers around AI, body weight were measured at different stages either before or after AI. We found that daily body weight gain was higher in the pregnant cows after AI. We also investigate correlation between body mass measured by shoulder height and body length, and conception rates, used (body length+ height)2 instead of height2 for body mass index (BMI), and found that relatively more BMI heifers (>55) showed higher conception rates. Finally, we estimated body weight by measuring should height (SH), heart girth (HG), and body length (BL); BW=3.93372*HG-2.90985*SH-0.021*BL. In addition, we observed that HG is most closely correlated with BW; y(BW) = 1.77355*x(HG), R² = 0.98881. In summary, we can determine the best timing of AI using body measurement and its application including BMI.

The KLF(Kruppel-like factors) are transcriptional regulators that including the C2H2 zinc-finger motif and regulation of cell growth, cell differentiation, and development. In this study, mRNA and total DNA was extracted from the meat around the backbone of 130 cattle that has economic traits score. The KLF11 gene was act on the center of the main pathway in associated with the development and growth of cells and the fat metabolism. Genetic polymorphism was detected by DNA sequencing. A total of four SNPs(G9047A, C9104G, G9131A, C9170T) were identified in exon 4 regions, statistical association analysis revealed that the G9131A polymorphism were significantly associated with marbling score. The real time quantitative PCR to investigated gene and miRNA expression patterns and identify the relation of economic trait in Korean Native Beef Cattle. Gene expression level linked to KLF11 genes was investigated by determining its association and variation with the economic traits in Korean Native Beef Cattle. The increment of KLF11 gene expresstion level is resulted in increasing marbling score(MS). We found that expression profile of KLF gene and mir-494 was contrast tendency because the lower expression ratio of gene was the higher marbling score rating was when we analyzed expression profile ofKLF gene and mir-494. Therefore, this result has useful in improvement of livestock in Korean cattle.

In experiment dealt with 240 Hanwoo (172 steers and 68 heifers) among the 456 cattle during growing and fattening period were used from 2004 to 2015. Growth period was divided into 6, 12, 18, and 24 months for steers and 6, 12, 18, and 43 months for heifers. The results showed that lying down, feeding, and drinking behavior increased according to the growth. In contrary, the time of locomotion behavior including standing and walking behavior were reduced. Aggressive behavior was observed to be mostly generated in the first 6-months. Thus the results showed that different growth period of Hanwoo steers can be used as management systems for breeding and disease management. It conclude that providing a comfortable living space is a useful management systems suppress stress to the minimum extent without giving unnecessary stress to the animals.

농장동물복지형 한우 사양관리를 통한 고품질의 쇠고기 생산을 목표로 세계적인 트랜드에 부합하는 축산선진국으로 나아가기 위하여 본 시험으로 우방내 사육공간의 넓이차이에 따른 한우의 사양성적, 행 동특성 및 혈액성상에 미치는 영향을 비교·분석해 보았다. 16개월령의 한우 54두(암소 미경산공태우 및 거세우 각각 27두)를 공시하여 성별에 따라 사육공간의 넓이차이를 주기 위해 4두(8m2), 3두 (10.67m2) 및 2두(16m2)로 처리하여 9개월간 각각 3반복으로 완전 임의배치 후 실시하였으며, 한우 사 양관리 프로그램에 따라 사료급여를 하였다. 데이터 수집방법으로 증체량, 사료요구율 및 혈액분석은 2 개월 간격으로 반복하여 측정하였으며, 행동패턴 수집은 복수개체 추적방법 및 선택적 행동관찰법으로 기상청에서 제공한 맑은 날을 선정하여 15일간 녹화 후 분석에 이용하였다. 본 시험의 결과로, 사양성 적(체중, 일당증체량 및 사료요구율)의 경우 미경산공태우와 거세우 각각 20개월령과 18개월령 이후부 터 2두와 3두 처리구에서 모두 유의적(p<0.05)으로 향상되었다. 행동특성으로는 미경산공태우의 2두 처 리구에서 행동시간으로 기립행동은 유의적(p<0.05)으로 낮고, 횡와 및 걷기행동은 유의적(p<0.05)으로 높았다. 행동빈도에서는 음수, 자기몸핥기, 긁기, 몸비비기, 투쟁행동에서 유의적(p<0.05)으로 적게 나 타났다. 거세우의 2두 및 3두 처리구에서는 미경산공태우와 유사한 경향을 나타냈으나 유의적(p<0.05) 으로 섭식시간은 증가하고, 다른소핥기 및 몸기대기의 빈도는 유의적(p<0.05)으로 적게 나타났다. 혈액 성상으로 미경산공태우와 거세우의 4두 처리구에서 적혈구가 유의적(p<0.05)으로 낮았고, 콜티졸은 유 의적(p<0.05)으로 높게 나타났다. 또한, 미경산공태우의 헤모글로빈과 거세우의 백혈구 수치에서는 모 두 2두 처리구에서 유의적(p<0.05)으로 각각 높고 낮은 것으로 분석되었다. 이상의 결과를 종합하면 사 양성적, 행동특성 및 혈액성상 등의 전체적인 내용을 토대로 우사의 여유 및 표준우사의 우방크기를 고 려할 때 한우의 사육공간에서 관행사육인 4두 처리구(두당 8m2) 보다 미경산공태우는 2두 처리구(두당 16m2), 거세우는 3두 처리구(두당 10.67m2)로 사육하는 것이 적절하다고 판단되며, 본 시험결과는 추후 복지형 한우의 사양관리를 위한 기초자료로 활용할 수 있을 것으로 판단된다.

In this study, the texture, nucleotide-related compounds, and sensory characteristics of dry-aged beef, made from third quality grade Hanwoo beef (Korean native cattle), were investigated according to aging periods. Loin cuts obtained from three different Hanwoos were dry-aged under the specific conditions (controlled temperatures, humidity, and air flow) during 0-7 weeks. With increasing aging periods, textures were significantly decreased after 5 weeks (hardness: from 13,264±2,033 to 7,112±1,743 g; chewiness: from 4,107±1,467 to 2,334±780) and four sensory characteristics were significantly increased after 3 weeks (texture: from 2.2±1.0 to 6.2±1.1; flavor: from 3.9±0.7 to 6.2±1.5; whole preference: from 2.5±0.8 to 6.3±1.3; purchase intention: from 3.7±1.4 to 6.0±1.5) except for color (p<0.05). On the other hand, the concentrations of IMP which is related to the umami flavor of beef were significantly decreased with increasing aging periods (from 40.44±5.97 at 0-week to 7.44±2.43 μg/ mg at 7-week) (p<0.05). These results obtained in this study could provide an useful data base for the quality control of dry-aging.

이 연구의 목적은 성염색체 특이적 초위성체마커의 대립 유 전자 다양성과 빈도를 조사하여 한국 재래소 3품종의 (칡소, 한우 그리고 제주흑우) 유전적 근연관계 및 특징을 조사하여 우리 고유 유전자원으로서의 가치를 구명하고자 하였다. 한국 재래소 3품종은 30개의 초위성체 마커에서 선별된 4개의 초 위성체 마커(INRA30, TGLA325, UMN0905 및 UMN0929) 를 이용하여 식별하였다. 대립 유전자의 다양성, 대립 유전자 빈도, 이형접합도 그리고 다형 정보량(PIC)을 산출 하였다. 칡 소, 제주 흑우와 한우에 대한 관찰 이형접합도의 평균은 각각 0.541, 0.406 및 0.61이었고 PIC의 평균은 각각 0.542, 0.377 그리고 0.6이었다. 제주흑우의 경우 칡소나 한우에 비해 낮은 이형접합도와 PIC를 보여준다. 이들 4개의 초위성체 마커를 이용하여 칡소, 제주흑우 그리고 한우의 품종을 구별에 이용 할 수 있다. 이러한 결과들로 볼 때 한국 재래소 3품종은 가 축유전자원으로서 중요한 가치를 지니고 있으며 이들 품종의 보존, 관리 및 활용에 중요한 기초자료로 이용될 것으로 사료 된다.