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        검색결과 3

        1.
        2020.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The fatty acid binding protein 4 (FABP4) gene plays an important role in lipid metabolism and homeostasis in adipocytes. The objective of this study was to analyze the association between a single nucleotide polymorphism (SNP), g.7516G>C, in the FABP4 gene and economic traits of Korean native cattle, Hanwoo. Primers were designed to target a region of the FABP4 gene between nucleotides 7417 and 7868 (AAFC01136716). The SNP, which was detected by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method using restriction enzyme MspA1I, was genotyped in 319 animals of Hanwoo steer population. Statistical analysis showed that the SNP genotype of the FABP4 gene significantly affected carcass weight (CW, p<0.01), longissimus muscle area (LMA, p<0.001), and marbling score (MS, p<0.001). GG allele of the SNP on 246 animals in a Wagyu × Limousin F2 reference population showed a higher MS (p<0.05) and subcutaneous fat depth (p<0.05) in previous report. But CC allele of the SNP showed greater values for MS, LMA, and CW in Hanwoo steers. These results suggest that the g.7516G>C SNP located in the FABP4 gene may affected differently depending on the cattle breed and can be used as a genetic selection marker in Korean native cattle.
        4,000원
        2.
        2020.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The long-tailed goral (Naemorhedus caudatus) is an endangered animal species in all its habitats worldwide, including South Korea. The imbalanced sex ratio in fragmented habitats is closely associated with extinction. Therefore, sex identification using wild animal samples would be necessary. However, only a few studies have been reported about the sex identification of gorals. In this study, we thus aimed at comparing the efficiency of sex identification using various goral sample types as templates and the amelogenin (AMEL) and DEAD-box polypeptide 3 (DDX3) genes as target sequences. We extracted DNA from goral feces, tissues, and blood samples, then amplified the AMEL (SE47/SE48 and SE47/SE53 primer pairs) and DDX3 genes for sex identification, comparing the goral DDX3X and DDX3Y target sequences to those in cattle. Our results indicated that the tissue- and blood sample-derived AMEL amplicons showed an unspecific band pattern containing the sex-specific band in the case of both primer pairs we used, whereas the DDX3 amplicon showed only the sex-specific band. In the case of the feces samples, only the sex-specific band was amplified using both the AMEL and DDX3 primer pairs. However, we found that the DDX3 amplicon exhibited a clearer band pattern than the AMEL amplicon. Then, we compared the DDX3X and DDX3Y target sequences between cattle and gorals. We found 5 and 8 nucleotide differences in the DDX3X and DDX3Y sequences, respectively. In conclusion, the DDX3 gene-related sex identification of the long-tailed goral appears to be more efficient and precise than the AMEL gene-related approach. This method could be used for the sex identification of the members of the Bovidae family.
        4,000원
        3.
        2013.05 서비스 종료(열람 제한)
        본 연구는 대상자의 수가 적고 연구기간이 짧아 연구결과를 일반화하기에 제한점이 있으나 원예치료프로그램이 우울증이 심한 중년여성들에게 매우 효과가 있는 것으로 나타났다. 감정표현 및 정서적 사회적 측면에서도 원예치료 참여전보다 긍정적인 반응이 나타났고, 사전, 사후 검사지를 통해 알 수 있듯이 우울감이 감소하고, 표정이 밝아지고, 보다 적극적으로 프로그램에 참여하고자 하는 의지가 강해졌다. 원예치료 프로그램 중 흙을 손으로 만지거나, 색깔 있는 돌이나 모래들을 식물들과 함께 연출하면서 신체활동과 정서적 활동을 통해서 우울감이 감소한 것으로 생각된다. 또한 프로그램시 만든 작품을 자신이 아닌 다른 누군가에게 선물을 주고 싶어 하고, 그런 반응들을 기회로 의사소통의 통로를 마련하여 가정과 사회 및 직장에서 중년여성들의 삶을 풍요롭게 하고, 타인과의 관계형성에 유대감이 강화되었고, 사회성이 향상되었다. 향후 원예프로그램이 좀 더 창의적이고 다양한 프로그램으로 개발되어 각 대상에 맞게 지속적인 치료활동으로 이루어진다면 직장생활을 하는 중년여성들뿐만 아니라 가족들에게도 매우 효과적인 치료가 가능할 것으로 보여진다.