해상특수교량은 특수한 환경적 조건뿐 아니라 고주탑의 구조형식, 보호재로 쌓여있는 케이블 등 특 수한 형식을 가지고 있어 일반적인 육안전검으로 안전점검을 할 수 없는 사각지대가 존재한다. 주탑의 외부 손상상태 및 케이블의 손상에 대해서는 정밀안전점검에서도 점검이 되지 않는 경우가 대부분이 므로 이에 대한 대책 마련이 시급하다. 또한 해상특수교량에 대한 전문적인 경험과 기술이 부족한 관 리자도 대상교량의 손상과 이상거동을 직관적으로 확인하고 판단할 수 있는 지원체계가 필요하다. 이 에 본 연구에서는 해상특수교량 고주탑에 대한 손상정보를 파악하기 위하여 드론의 자동비행 기술을 개발하고 이를 이용하여 주탑 외부 균열 손상에 대한 안전점검을 실시하고 이를 분석하였다.

최근 지방정부는 지역경제 활성화를 위한 관광객 유치를 위해 다양한 모양 구조를 가지는 출렁다리 를 경쟁적으로 건설하고 있다. 이러한 출렁다리의 안전관리는 자체 가이드라인이나 국토교통부에서 발 간한 매뉴얼을 통해 관리되고 있으나 실제로는 관광이나 공원 관리 부서에서 관리하고 있어 안전관리 에 소홀한 실정이다. 국토교통부에서는 제3종시설물에 대한 안전점검 매뉴얼 발간하고 안전등급 평가 기준을 강화하여 기존 3분위(양호, 보통, 불량) 평가기준을 5분위(우수, 양호, 보통, 미흡, 불량) 평가체 계로 개정하였으며, 기존 세부지침과 평가기준도 일원화 하였다. 그러나 출렁다리의 경우 제1종, 제2 종 시설물인 교량과 달리 점검 방식 및 체크리스트가 달라져야 하므로 출렁다리 정기안전점검을 위한 점검 수준에 맞게 개선되어야 한다. 본 연구에서는 출렁다리에 안전전검에 체계 개선을 위한 출렁다리 정기안전점검 매뉴얼(안)을 제시하였다.

Hexagonal bolt, nut, fittings, and high-pressure valves with special alloy play an important role in many industrial products, for instance, such as semiconductor facilities, hydrogen stations and fuel cell electric vehicles. The purpose of this study is to investigate the structural stability of turning wheel using the reaction force of roller in variable hexagonal rolling die. As the results, the bearing groove had the possibility of damage in turning wheel, especially, in case of Bottom condition. Furthermore, the turning wheel showed structural instability by using safety factor but structural stability using strength, respectively, as a safety criterion.

Hexagonal bolt, nut, fittings, and high-pressure valves with special alloy play an important role in many industrial products, for instance, such as semiconductor facilities, hydrogen stations and fuel cell electric vehicles. The purpose of this study is to predict the reaction force of roller in drawing system. Numerical analysis was conducted to obtain data for designing the turning wheel, which is key parts in drawing system, using the reaction force of roller. As the results, the reaction force of X axis direction was about 9~20 times larger than that of Z axis direction and the reaction force of Y axis direction was negligible. The maximum reaction force of roller was the case of 4 stage and the numerical results in this study could be helped for designing the mechanical parts of variable hexagonal rolling die.

The long-tailed goral (Naemorhedus caudatus) is classified as 'vulnerable' by the International Union for Conservation of Nature and has been designated an endangered species requiring conservation and management in Korea. The sex determination region of the Y (SRY) gene is a useful marker for the study of paternal lineages; however, the SRY gene of the goral has not yet been sequenced. In this study, the nucleotide sequence of the SRY gene of long-tailed gorals was determined based on the sequence of the SRY gene of goats (Capra hircus). The obtained sequences were aligned with those of other species in the Bovidae family. The long-tailed goral SRY gene comprised 720 base pairs, and its nucleotide and protein sequences were identical to those of goats, sheep (Ovis aries), and cattle (Bos taurus) by 96%, 97%, and 93%, respectively. The results of phylogenetic insights obtained from this study constitute important references for genetic diversity and pedigrees studies of male long-tailed gorals and closely related species.

본 연구는 아세트아미노펜, 비타민 C, 무수 구연산으로 구성된 해열진통 및 염증 완화제, 피버닐산의 사료첨가가 구제역(FMD) 백신의 접종으로 야기 될 수 있는 돼지의 부작용에 대한 감소 효과를 확인하기 위해 실시되었다. 8-9주령의 돼지(LYD) 30마리를 공시, 대조군(n=10), 구제 역 백신 접종군(n=10; T1), 구제역 백신+피버닐 산 투여군 (n=10; 백신 접종 후 5일 간 0.2% 피버닐 산 경구 투여, T2), 3개 그룹으로 배치하여 시험을 진행하였다. 각 시험 구에서 총 14일 동안 체중, 혈액학치, 혈액화학치, FMD 항체 및 스트레스 지표의 변화를 관찰했으며, 총 증체량 은 T1(11.13±1.33kg)에 비해 T2(13.76±1.73kg)에서 유의적 으로 증가하였다(p<0.001). 혈액학치 및 혈액화학치 분석 결과, 백신을 접종한 두 처리군에서 유의한 차이는 없었 다. FMD 항체 역가는 T1(89.1±7.9)에 비해 T2(91.9±5.6)에 서 증가하는 경향을 보였으나 통계적 유의성을 나타나지 않았다. T2 그룹의 코르티솔, TNF-α 및 CRP와 같은 스 트레스, 염증 지표 물질은 구제역 백신 접종 후 3일에서 5 일 사이에 각각 310.31±44.11ng/mL, 59.05±11.88pg/mL, 29.05±2.84ng/mL으로 T1(361.58±58.74ng/mL, 74.47±19.26 pg/mL, 34.21±5.34ng/mL)보다 유의적으로 낮게 측정되 었다. 시험 14일째에 육안 및 조직병리학적 분석 결과, 주 사 부위의 염증 반응은 T2에서 적게 관찰되었으며 염증세 포 침윤 및 섬유조직이 증식된 육아종성 염증 또한 T2에 서 적게 관찰되었다. 본 연구결과로 미루어 보아 피버닐 산의 0.2% 경구 투여는 체중감소, 발열 등의 임상증상을 감소시키는 효과가 있으며, 접종 부위의 염증반응을 억제 하여 구제역 백신 접종의 부작용에 대한 스트레스를 경감 시키는 것으로 판단된다. 따라서 백신 접종 후 피버닐 산 의 0.2% 경구 투여는 돼지에서 구제역 백신 접종과 관련 된 부작용을 줄이는 데 효과적인 것으로 사료된다.

This study evaluated whether fevernill, which is primarily composed of acetaminophen, vitamin C, and anhydrous citric acid, is effective in relieving stress caused by foot-and-mouth disease virus (FMDV) vaccination in calves. Three-month-old calves from a farm in Korea were randomly assigned to one of three groups (n = 10 per group: control [untreated], group A [FMDV vaccination], and group B [FMDV vaccination + addition of fevernill 0.2% to feed for five days after vaccination]). Body weights, antibody formation, clinical symptoms, serum parameters were measured in the 14 days following vaccination. Total weight gain and average daily gain during the experiment period were group A (12.00±1.15 kg, 0.86±0.08 kg/day) compared to group B (13.57±0.98 kg, 0.97±0.07 kg/day) was significantly improved (p<0.05). There was no significant difference in the formation of antibodies against the FMDV vaccine between group A and group B, though the antibody value of group B tended to be higher than that of group A. The pre-inflammatory cytokine TNF-α value was 74.47±19.26 pg/ml in group A and 59.05±11.88 pg/ml in group B on the 5th day of the experiment, which was significantly lower in group B than in group A (p<0.05). Also, cortisol concentrations were significantly lower in group B than in group A (p<0.05). In conclusion, the feeding of fevernil is judged to be helpful in mitigating the side effects caused by FMD vaccination, and thus it is thought to be able to prevent the decrease in productivity caused by vaccination.

This study is an experimental study to evaluate the vehicle-protection performance of the reinforced F section of a concrete median barrier. To reinforce the strength performance and durability of the F section, the design strength of concrete was increased and a wire mesh was added. The reinforced F section was tested in an actual collision to verify the protection performance of the SB4 class(Impact Severity: 160kJ) concrete barrier. In the truck-crash test for strengthperformance evaluation, no damage or no sufficient scattering occurred to cause damage to a third party. In addition, the truck smoothly moved through an escape box after the collision. THIV and PHD, which are criteria for evaluating the occupant-protection performance, were measured at 75% and 64% of the limit, respectively, and were confirmed to provide good occupant-protection performance. The vehicle rotations after the collision were 12%–19% of the allowed limits.

PURPOSES : This study evaluates the mechanical properties of high-viscosity polymer-modified asphalt binders using PG and MSCR tests. METHODS : Using the Superpave asphalt binder performance grade (PG) and multi-stress creep recovery (MSCR) test methods, the linear (dynamic shear modulus, stiffness, and viscosity) and non-linear properties — i.e., non-recoverable compliance (Jnr) and recoverable shear strain % recovery — of 16 different types (SBS, SIS, PE, PP) of polymer-modified asphalt binders were assessed. RESULTS : Based on the viscosity testing results. Most PG 82 binders did not meet the criterion of 3 Pa ·s. This indicates that they need to increase the mixing and compaction temperatures to reduce the high viscosity. The MSCR % Jnr results demonstrated that PG 76, PG 82–22, and PG 82-28/34 binders were below 1.0, 0.5, and 0.2, respectively. In addition, the MSCR % recovery results showed that PG 76, PG82-22, and PG82-28/34 were above 35 %, 55 %, and 80 %, respectively. CONCLUSIONS : It was found that the % Jnr decreased with an increase in the high-temperature PG of the asphalt binder, whereas the % recovery increased as the low-temperature PG of the binder increased.

The fatty acid binding protein 4 (FABP4) gene plays an important role in lipid metabolism and homeostasis in adipocytes. The objective of this study was to analyze the association between a single nucleotide polymorphism (SNP), g.7516G>C, in the FABP4 gene and economic traits of Korean native cattle, Hanwoo. Primers were designed to target a region of the FABP4 gene between nucleotides 7417 and 7868 (AAFC01136716). The SNP, which was detected by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method using restriction enzyme MspA1I, was genotyped in 319 animals of Hanwoo steer population. Statistical analysis showed that the SNP genotype of the FABP4 gene significantly affected carcass weight (CW, p<0.01), longissimus muscle area (LMA, p<0.001), and marbling score (MS, p<0.001). GG allele of the SNP on 246 animals in a Wagyu × Limousin F2 reference population showed a higher MS (p<0.05) and subcutaneous fat depth (p<0.05) in previous report. But CC allele of the SNP showed greater values for MS, LMA, and CW in Hanwoo steers. These results suggest that the g.7516G>C SNP located in the FABP4 gene may affected differently depending on the cattle breed and can be used as a genetic selection marker in Korean native cattle.

The long-tailed goral (Naemorhedus caudatus) is an endangered animal species in all its habitats worldwide, including South Korea. The imbalanced sex ratio in fragmented habitats is closely associated with extinction. Therefore, sex identification using wild animal samples would be necessary. However, only a few studies have been reported about the sex identification of gorals. In this study, we thus aimed at comparing the efficiency of sex identification using various goral sample types as templates and the amelogenin (AMEL) and DEAD-box polypeptide 3 (DDX3) genes as target sequences. We extracted DNA from goral feces, tissues, and blood samples, then amplified the AMEL (SE47/SE48 and SE47/SE53 primer pairs) and DDX3 genes for sex identification, comparing the goral DDX3X and DDX3Y target sequences to those in cattle. Our results indicated that the tissue- and blood sample-derived AMEL amplicons showed an unspecific band pattern containing the sex-specific band in the case of both primer pairs we used, whereas the DDX3 amplicon showed only the sex-specific band. In the case of the feces samples, only the sex-specific band was amplified using both the AMEL and DDX3 primer pairs. However, we found that the DDX3 amplicon exhibited a clearer band pattern than the AMEL amplicon. Then, we compared the DDX3X and DDX3Y target sequences between cattle and gorals. We found 5 and 8 nucleotide differences in the DDX3X and DDX3Y sequences, respectively. In conclusion, the DDX3 gene-related sex identification of the long-tailed goral appears to be more efficient and precise than the AMEL gene-related approach. This method could be used for the sex identification of the members of the Bovidae family.

In the case of foot-and-mouth disease (FMD), there is a great deal of impact on the national economy due to the disposal of diseases, the cost of disease control such as vaccination, reduction of productivity, and restriction of international trade of livestock products. Therefore, appropriate diagnostic methods for sensitive, accurate and rapid identification of virus serotypes are continuously required in terms of early prevention of FMD. This study was conducted to confirm the feasibility of immuno-PCR diagnostic method for the more sensitive detection of Korean FMD virus (FMDV). We synthesized a partial FMD type A viral gene. Protein antigen, monoclonal and polyclonal antibodies of FMDV were cloned, expressed and purified and then magnetic particles were attached to polyclonal antibodies and and oligomers to monoclonal antibodies for the immnuno-PCR. We confirmed the antigen-antibody and oligomer reaction using ELISA, Western blot, and real-time PCR. These results show that Korean FMDV can be detected by using difference of Ct values between positive group and negative group using immuno-PCR.. The results of this study also suggest that this technique will be the basis of the diagnosis method to detect Korean FMDV more sensitively in the future.