Bovine rotavirus (BRV) is one of the common gastrointestinal diseases that can occur in calves, causing severe diarrhea. This study was conducted to investigate the effects of oral administration of single-domain antibodies, single variable domain of heavy chain of HCAb (VHH), on the prevention and treatment of rotavirus-induced diarrhea in calves. Thirty calves were divided into five experimental groups (negative control, positive control, group A, group B and group C). Except for the negative control group, the experimental groups were inoculated with BRV on the 4th day after birth. The VHH supplementation groups were fed with a substitute milk containing different concentrations of VHH (1%, 2.5% and 7.5%). Changes in body weight, clinical symptoms, serum antibodies, and virus detection in feces were observed for a total of 10 days in each experimental group. The results showed a higher survival rate in the VHH supplementation groups compared to the positive control group. Clinical symptoms caused by rotavirus were alleviated in the VHH supplementation groups. The detection rate of rotavirus in feces also decreased. Gross findings indicated that the severity of disease in the VHH supplementation groups was milder than that in the positive control group. Therefore, VHH could be proposed as a useful alternative for the prevention and treatment of rotavirus-induced diarrhea in calves.

Ⅰ. 서론
Ⅱ. 재료 및 방법
    1. 공시재료
    2. 공시동물
    3. 사양관리
    4. 공격접종
    5. 예방 및 치료 효과 지표
    6. 통계처리
Ⅲ. 결과
    1. 체중
    2. 임상증상
    3. 분변 내 BRV 농도 및 혈중 면역항체(IgG) 농도
    4. 혈액검사 소견
Ⅳ. 고찰
Ⅴ. 요약
Ⅵ. 참고문헌

• 양송이(㈜애드바이오텍 학술팀 팀장, 강원대학교 수의과대학 및 동물의과학연구소 연구원) | Song-Yi Yang (Manager ADBIOTECH IND. CO., LTD., Chuncheon 24398, Korea, Researcher, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea)
• 김양희(강원대학교 수의과대학 및 동물의과학연구소 연구원) | Yang-Hee Kim (Researcher, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea)
• 이성진(강원대학교 동물응용과학과 교수) | Sung-Jin Lee (Professor, Department of Applied Animal Science, Kangwon National University, Chuncheon 24341, Korea)
• 한정희(강원대학교 수의과대학 및 동물의과학연구소 교수) | Jeong-Hee Han (Professor, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea) Corresponding author