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한우 체외수정란의 수정란이식에 관한 연구 KCI 등재

Studies on the Embryo Transfer of in Vitro Fertilized Embryos in Hanwoo

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동물자원연구 (Annals of Animal Resources Sciences)
강원대학교 동물자원공동연구소 (Institute of Animal Resources Kangwon National University)
초록

These studies were carried out to improve the reproductive efficiency through embryos transfer of Hanwoo IVM/IVF embryos. Following routine IVM/IVF procedure, Oocytes and zygotes were cultured for 40 to 44 h in CR1aa medium with BSA. Then 2 to 8-cell embryos were removed the cumulus cell and were cultured in CR1aa medium containing 10% fetal bovine serum and 2.5 mM taurine in 5% O2 and 5% CO2 at 38.5℃. The fresh and frozen thawed embryos of the morulae and blastocysts cultured for 6 to 9 days in vitro were transferred into recipients. The pregnancy rates of the blastocyst produced for 6, 7, 8, and 9 days in in vitro culture were 41.9, 48.6, 57.9 and 47.4%, respectively. In the developmental stage, pregnancy rates of early blastocysts (41.7%), blastocysts (57.6%) and expanded blastocysts (50.0%) were higher than that of morulae stage (00.0%). Fresh and frozen embryos on the pregnancy rates were 48.9 and 50.0%, respectively. These results indicate that the pregnancy rate after transfer were affected on embryonic stage of in vitro embryos and in vitro culture periods.

목차
ABSTRACT   Ⅰ. 서론   Ⅱ. 재료 및 방법    1. 한우 난포란의 체외성숙 및 수정    2. 체외수정란의 체외배양    3. 체외수정란의 동결보존    4. 한우 체외수정란의 이식    5. 임신감정   Ⅲ. 결과    1. 체외수정란의 체외발육 및 체외배양일수에 따른 수태율    2. 체외수정란의 발육상태에 따른 수태율과 이식 위치에 따른 수태율    3. 신선 및 동결란에 따른 이식 후 수태율   Ⅳ. 고찰   Ⅴ. 적요   Ⅵ. 인용문헌
저자
  • 조명현(강원대학교 동물자원과학대학) | Myung-Hyun Cho
  • 박동헌(강원대학교 동물자원과학대학) | Dong-Heon Park
  • 장현용(강원대학교 동물자원과학대학) | Hyun-Young Jang
  • 정희태(강원대학교 동물자원과학대학) | Hee-Tae Cheong
  • 박춘근(강원대학교 동물자원과학대학) | Choon-Keun Park
  • 김정익(강원대학교 동물자원과학대학) | Choung-Ik Kim
  • 양부근(강원대학교 동물자원과학대학) | Boo-Keun Yang Corresponding Author