The Oct-4 (octamer-4), a member of the POU family transcription factor, is expressed in early mouse embryogenesis and in pluripotent embryonic stem (ES) lines. Oct-4 expression is thought to remain confined to the germline after gastrulation in the embryo. Therefore, the study was designed to, study the location of Oct-4 protein in the ovaries, placenta and testis of Korean native cattle (Hanwoo). Expression of Oct-4 mRNA in the ovaries and placenta of bovine was confirmed by RT-PCR and immunohistochemical analysis. Oct-4 was expressed in granulosa, thecal cells irrespective of the shape and size of follicles and endometerium of Korean native cattle (Hanwoo). Expression of Oct-4 was profound in all the tissues of Korean native cattle (Hanwoo) suggestung their role in them. Oct-4 localization and expression could contribute to further developmental studies in Korean native cattle (Hanwoo).

ABSTRACT   Ι. 서론   Ⅱ. 재료 및 방법    1. 공시 동물    2. 공시 재료의 처리    3. RNA 추출 및 RT-PCR    4. 면역조직 염색법(immunohistochemistry)   Ⅲ. 결과 및 고찰    1. 한우의 태반, 난소 및 정소에서의 Oct-4 mRNA 발현    2. 한우의 난소와 태반에서의 Oct-4 단백질 발현 양상   Ⅳ. 요약   사사   Ⅴ. 인용문헌

• 김현(국립축산과학원 가축유전자원시험장) | Hyun Kim
• 최재혁(국립축산과학원 동물바이오공학과) | Jae-Hyuk Choi
• 김성우(국립축산과학원 가축유전자원시험장) | Sung-woo Kim
• 양보석(국립축산과학원 가축유전자원시험장) | Bohsuk Yang
• 고응규(국립축산과학원 가축유전자원시험장) | Yeoung-Gyu Ko Corresponding Author