논문 상세보기
pp.136-141
고령화 사회로 접어들면서 만성 신부전증 환자는 증가되고 있으며, 저단백질 식품에 대한 수요도 증가되고 있다. 중국173/주남벼 재조합 집단을 이용하여 저Glutelin 고품질 벼 품종개발의 육종효율 증진을 위해 DNA마커의 이용성을 검토하였으며, 밥맛향상을 위해 미질관련 형질들과 상관분석을 수행하였다. 중국173호/주남벼 재조합 집단에서 SDS PAGE결과 얻어진 저Glutelin 표현형과 functional DNA marker의 유전자형이 일치하여 DNA marker를 이용한 선발이 가능함을 확인하였다. 밥맛향상을 위해 미질관련 형질들과 상관분석을 수행한 결과, 윤기값과 Amylose 함량은 유의한 부의 상관을 나타내었으며, 단백질 함량은 윤기값에 많은 영향을 미치지 않았다. 반면, 단백질 조성에 따른 저Glutelin 계통군과 정상계통군의 비교에서 저Glutelin 계통군은 정상군에 비해 유의하게 윤기값이 낮았으며, 저Glutelin-저Amylose 계통군과 정상계통군은 대등한 윤기값을 유지하였다. 따라서 고품질 저Glutelin 품종개발을 위해 저Amylose 유전자의 활용이 바람직한 것으로 판단되었다. 이들 분석결과와 주요 농업적 특성을 고려하여 저Glutelin-저Amylose인 유망계통 밀양262호를 육성하였으며, 금후 고품질 저Glutelin 품종개발에 활용될 것으로 기대된다.
In chronic renal failure, to reduce the burden on the kidneys, it is necessary to provide patients with therapy based on a low-protein diet. This study was conducted to improve eating quality for developing low easy to-digest protein rice variety with Lgc1 gene. Two hundred twenty six recombinant inbred lines (RILs) were used, which were derived from the cross between Chugoku173, a low glutelin rice variety developed in Japan, and Junambyeo, a high quality rice variety in Korea. We investigated glutelin phenotype using SDS PAGE and its genotype by functional DNA marker. As results of them were consistent, this DNA marker is expected to use of MAS for the Lgc1- mediated low glutelin variety. The eating quality of rice measured by Toyo meter (GMT) showed significantly negative correlation with amylose content, but there was no significance with protein content in this population. Dividing the RILs into normal amylose group (app. 19%) and low amylose group (app. 13%), the eating quality estimated by GMT was generally lower in low glutelin lines, but that of low amylose group was higher than that of normal amylose group. On the basis of several results, it is supposed that low amlyose gene can be used to breed the low glutelin rice variety with high eating quality. Through the analysis of agronomic traits related to eating quality, Milyang262, low glutelin and amylose rice variety, were developed.
