The effects of medium, hormones, and PFP on the proliferation of red callus in leaf tissue cultures of Garden orach(Atriplex hortensis L.) was investigated. As a result,88% of leaf tissues formed eallus on MS nledium containing 2mg/l 2,4-D. Fresh weight of callus was higher on MS medium than on Bsand NN media. It was also found that 2, 4-D was more effective than Dicamba and Picloram. The op-timum concentrations of hormones for callus proliferation depended on culture media. Isolated red cal-lus grew markedly both on MS medium supplemented with 1-2mg/l 2, 4-D and Bs medium contain-ing 2-4111g/l 2,4D. Callus proliferated on B5 and NN media containing Dicabma Img/l as well as onthe same media containing 2mg/l Picioram. The addition of PFP concentrations of 2, 5, and 40mg/ lrcspectiely to culture medium caused increase of callus fresh weight, especially under light condition.

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