To determine the optimal media conditions for the detection of the cellulolytic activity in Ganoderma neo- japonicum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35℃. On the whole, the best protocol called for Ganoderma neo-japonicum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at 25℃ for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma neo-japonicum.

• Woo-Sik Jo(Department of Agricultural Environment, Gyeongbuk Province Agricultural Technology Administration, Daegu 702-320, Korea)
• Ha-Na Park( Department of Agricultural Environment, Gyeongbuk Province Agricultural Technology Administration, Daegu 702-320, Korea) | Ha-Na Park
• Doo-Hyun Cho( Department of Agricultural Environment, Gyeongbuk Province Agricultural Technology Administration, Daegu 702-320, Korea) | Doo-Hyun Cho
• Seong-Yong Choi( Department of Agricultural Environment, Gyeongbuk Province Agricultural Technology Administration, Daegu 702-320, Korea) | Seong-Yong Choi
• Young-Bok Yoo( Mushroom Research Division Department of Herbal Crop Research National Inst. of Horicultural & Herbal Science, RDA, Suwon 404-707, Korea) | Young-Bok Yoo