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The antioxidant activities of the methanol extract from Naematoloma sublateritium ,and its subfractions were assessed: ABTS+-radical-cation scavenging, 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing- and nitrite scavenging activity. The chloroform subfraction exhibited the strongest antioxidant activity except nitrite scavenging activity in which water subfraction showed the highest activity (50% scavenging at 48 mg/ml). The trolox equivalent antioxidant capacity (TEAC) of the chloroform subfraction was 649.40 μ mol/g of sample. This fraction also scavenged DPPH radical by 50% at 406.59 μg/ml. The highest ferric reducing activity was found in same subfraction (299.24 μmol FeSO4• 7H2O/g). Chloroform and ethylacetate fractions exhibited the highest contents of total phenolic compounds, with ferulic acid equivalents of 31.66 and 48.57 mg/g, respectively. The amount of flavonoid was at maximum in chloroform subfraction (18.66mg/g). The level of phenolic compounds may be correlated with the antioxidant activities of the subfractions. Therefore, the phenolic compounds in the subfractions were further identified by mass spectrometry using LC-MS/MS.
