The antioxidant activities of the methanol extract from Naematoloma sublateritium ,and its subfractions were assessed: ABTS+-radical-cation scavenging, 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing- and nitrite scavenging activity. The chloroform subfraction exhibited the strongest antioxidant activity except nitrite scavenging activity in which water subfraction showed the highest activity (50% scavenging at 48 mg/ml). The trolox equivalent antioxidant capacity (TEAC) of the chloroform subfraction was 649.40 μ mol/g of sample. This fraction also scavenged DPPH radical by 50% at 406.59 μg/ml. The highest ferric reducing activity was found in same subfraction (299.24 μmol FeSO4• 7H2O/g). Chloroform and ethylacetate fractions exhibited the highest contents of total phenolic compounds, with ferulic acid equivalents of 31.66 and 48.57 mg/g, respectively. The amount of flavonoid was at maximum in chloroform subfraction (18.66mg/g). The level of phenolic compounds may be correlated with the antioxidant activities of the subfractions. Therefore, the phenolic compounds in the subfractions were further identified by mass spectrometry using LC-MS/MS.

Antioxidant activities of Hericium erinaceum was in vestigated in subfractions of methanol extract such as hexane, chloroform, ethylacetate, butanol and water subfractions. Chlorofrom fraction exhibited the highest antioxidant activity and showed the highest level of total phenolic contents. The level of total phenolics may be correlated with the antioxidant activities of the subfractions. Therefore, the phenolic compound in the chloroform subfractions exhibiting high antioxidant acticity was identified by mass spectrometry using LC-MS/MS. In addition, hot water extract of this mushroom were tested for antimutagenic activity by Ames Salmonellaassay.Thisextractshowed inhibitiory effect on the mutagenesis induced in Salmonella typhimurium strain TA100 and TA98 by the directly actingmutagen 4-nitroquinoline-1-oxide(0.15μg/plate) and sodium azide (0.5μg/plate). The extract also inhibited mutagenesis induced in Salmonella typhimurium strain TA100 and TA98 by the indirectly actingmutagen 2-aminoanthracen(2μg/plate) at 5000μg/plate and 200 μg/plate, respectively.