This study was conducted to investigate the anti-bacterial effect of chlorine dioxide (ClO2) on extending thevase life of Gerbera jamesonii ‘Jenny’ which was first bredin Korea in 2012. Compared to commercialized biocides,ClO2 had been proved significantly effective for extendingthe vase life of cut gerbera flowers, equally with sodiumhypochlorite, silver nitrate, and 8-hydroxyquinoline sulfate.No bacteria were detected in the vase solutions treated withClO2 and these commercialized biocides on day 6 aftertreatment. But there were no significant differences on thevase life among the three ClO2 concentrations: 2, 5, and10μL•L−1. The ClO2 concentrations were rapidly decreasedon day 2 after treatment regardless of peduncle existence.Nevertheless, the antibacterial effect of ClO2 was main-tained up to day 6, indicating that the initial antibacterialeffect of ClO2 was a key factor for extending the vase life inthe gerbera flowers. The study results verified the antibacte-rial effect of ClO2 on prolonging the vase life of Gerberajamesonii ‘Jenny’ and demonstrated that appropriate ClO2application can prolong the postharvest life of cut gerberasvia its antimicrobial effect.